##gff-version 3
##sequence-region BBa_I8031 1 3537
BBa_I8031	.	terminator	3409	3488	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8031	.	stem_loop	3420	3463	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8031	.	engineered_region	3409	3488	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8031	.	terminator	2509	2549	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8031	.	stem_loop	2516	2535	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8031	.	stop_codon	2542	2542	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8031	.	polyA_site	2536	2549	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8031	.	engineered_region	2509	2549	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8031	.	ribosome_entry_site	774	785	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8031	.	conserved_region	778	781	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8031	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I8031	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8031	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8031	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8031	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8031	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8031	.	CDS	81	740	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I8031	.	tag	701	734	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I8031	.	engineered_tag	741	765	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I8031	.	CDS	84	700	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I8031	.	ribosome_entry_site	1581	1592	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8031	.	conserved_region	1585	1588	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8031	.	terminator	2421	2500	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8031	.	stem_loop	2432	2475	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8031	.	engineered_region	2421	2500	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8031	.	promoter	2558	2612	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I8031	.	engineered_region	2558	2612	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I8031	.	start_codon	2610	2610	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I8031	.	promoter	2599	2604	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I8031	.	promoter	2577	2582	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I8031	.	non_covalent_binding_site	2558	2577	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I8031	.	CDS	792	1547	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I8031	.	point_mutation	1283	1283	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I8031	.	engineered_region	792	1547	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I8031	.	engineered_region	792	793	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I8031	.	CDS	792	1541	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I8031	.	point_mutation	965	965	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I8031	.	engineered_tag	1548	1572	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I8031	.	engineered_region	2639	3400	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I8031	.	sequence_alteration	2880	2880	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I8031	.	engineered_region	2639	3400	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I8031	.	tag	3356	3394	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I8031	.	stop_codon	3395	3400	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I8031	.	CDS	2639	3400	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I8031	.	terminator	3497	3537	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8031	.	stem_loop	3504	3523	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8031	.	stop_codon	3530	3530	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8031	.	polyA_site	3524	3537	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8031	.	engineered_region	3497	3537	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8031	.	CDS	1599	2387	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_I8031	.	CDS	1599	2348	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_I8031	.	tag	2349	2381	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_I8031	.	engineered_region	1599	2387	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_I8031	.	engineered_tag	2388	2412	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_I8031	.	start_codon	1599	1601	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_I8031	.	stop_codon	2382	2387	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_I8031	.	ribosome_entry_site	2621	2632	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8031	.	conserved_region	2625	2628	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8031	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8031	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I8031
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctg
ttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctat
tttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggtt
ttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttata
ttattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgc
tgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaa
ggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattg
agacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatt
tgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgtt
ttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacg
ctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcga
ataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctg
gtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagc
tgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcct
gcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccag
gagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtga
accgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacag
ccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggac
ggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccact
acctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgc
cgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata