LOCUS       BBa_I8032               2701 bp    DNA     linear       26-Dec-2024
DEFINITION  Booperator aiia test 3: effect of tetR on production of RhlR
ACCESSION   BBa_I8032
VERSION     BBa_I8032.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..700
                     /label=tetR
     misc_feature    701..734
                     /label=SsrA
     misc_feature    741..765
                     /label=Help:Barcodes
     CDS             81..740
                     /label=BBa_C0040
     misc_feature    778..781
                     /label=conserved
     RBS             774..785
                     /label=BBa_B0034
     CDS             792..1514
                     /label=rhlR
     misc_feature    1515..1547
                     /label=LVA
     misc_feature    1554..1578
                     /label=Help:Barcodes
     CDS             792..1553
                     /label=BBa_C0071
     stem_loop       1598..1641
                     /label=stem_loop
     misc_feature    1587..1666
                     /label=BBa_B0010
     terminator      1587..1666
                     /label=BBa_B0010
     stem_loop       1682..1701
                     /label=T7 TE
     misc_feature    1708..1708
                     /label=stop
     polyA_site      1702..1715
                     /label=polya
     misc_feature    1675..1715
                     /label=BBa_B0012
     terminator      1675..1715
                     /label=BBa_B0012
     misc_feature    1724..1743
                     /label=RhlR
     promoter        1742..1747
                     /label=-35
     promoter        1765..1770
                     /label=-10
     misc_feature    1776..1776
                     /label=start
     promoter        1724..1776
                     /label=BBa_R0071
     misc_feature    1789..1792
                     /label=conserved
     RBS             1785..1796
                     /label=BBa_B0034
     CDS             1803..2564
                     /label=YFP (LVA)
     misc_feature    2559..2564
                     /label=2
     misc_feature    2520..2558
                     /label=SsrA
     misc_feature    2044..2044
                     /label=A (Q->L)
     misc_feature    1803..2564
                     /label=BBa_E0032
     misc_feature    1803..2564
                     /label=BBa_E0032
     stem_loop       2584..2627
                     /label=stem_loop
     misc_feature    2573..2652
                     /label=BBa_B0010
     terminator      2573..2652
                     /label=BBa_B0010
     stem_loop       2668..2687
                     /label=T7 TE
     misc_feature    2694..2694
                     /label=stop
     polyA_site      2688..2701
                     /label=polya
     misc_feature    2661..2701
                     /label=BBa_B0012
     terminator      2661..2701
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
      121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg
      781 agaaatacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg
      841 agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc
      901 gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga
      961 agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact
     1021 acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga
     1081 gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct
     1141 gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg
     1201 cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt
     1261 gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca
     1321 acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga
     1381 gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca
     1441 agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg
     1501 cgctgggtct catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat
     1561 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1621 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1681 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtcctgtg aaatctggca
     1741 gttaccgtta gctttcgaat tggctaaaaa gtgttctact agagaaagag gagaaatact
     1801 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1861 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     1921 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1981 ccctcgtgac caccttcggc tacggcctgc aatgcttcgc ccgctacccc gaccacatga
     2041 agctgcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2101 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2161 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2221 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2281 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2341 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2401 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2461 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     2521 ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat
     2581 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2641 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2701 a
//