##gff-version 3 ##sequence-region BBa_I8032 1 2701 BBa_I8032 . terminator 2661 2701 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I8032 . stem_loop 2668 2687 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I8032 . stop_codon 2694 2694 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I8032 . polyA_site 2688 2701 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I8032 . engineered_region 2661 2701 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I8032 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_I8032 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_I8032 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_I8032 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_I8032 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_I8032 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_I8032 . ribosome_entry_site 1785 1796 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8032 . conserved_region 1789 1792 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8032 . terminator 2573 2652 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I8032 . stem_loop 2584 2627 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I8032 . engineered_region 2573 2652 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I8032 . ribosome_entry_site 63 74 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8032 . conserved_region 67 70 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8032 . ribosome_entry_site 774 785 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I8032 . conserved_region 778 781 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I8032 . promoter 1724 1776 . + 0 ID=BBa_R0071;Name=BBa_R0071 BBa_I8032 . non_covalent_binding_site 1724 1743 . + 0 ID=annotation297102;Name=RhlR;Parent=BBa_R0071 BBa_I8032 . promoter 1765 1770 . + 0 ID=annotation297104;Name=-10;Parent=BBa_R0071 BBa_I8032 . promoter 1742 1747 . + 0 ID=annotation297103;Name=-35;Parent=BBa_R0071 BBa_I8032 . start_codon 1776 1776 . + 0 ID=annotation297105;Name=start;Parent=BBa_R0071 BBa_I8032 . terminator 1675 1715 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I8032 . stem_loop 1682 1701 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I8032 . stop_codon 1708 1708 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I8032 . polyA_site 1702 1715 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I8032 . engineered_region 1675 1715 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I8032 . terminator 1587 1666 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I8032 . stem_loop 1598 1641 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I8032 . engineered_region 1587 1666 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I8032 . engineered_region 1803 2564 . + 0 ID=BBa_E0032;Name=BBa_E0032 BBa_I8032 . sequence_alteration 2044 2044 . + 0 ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032 BBa_I8032 . engineered_region 1803 2564 . + 0 ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032 BBa_I8032 . tag 2520 2558 . + 0 ID=annotation2160;Name=SsrA;Parent=BBa_E0032 BBa_I8032 . stop_codon 2559 2564 . + 0 ID=annotation2159;Name=2;Parent=BBa_E0032 BBa_I8032 . CDS 1803 2564 . + 0 ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032 BBa_I8032 . CDS 792 1553 . + 0 ID=BBa_C0071;Name=BBa_C0071 BBa_I8032 . tag 1515 1547 . + 0 ID=annotation306523;Name=LVA;Parent=BBa_C0071 BBa_I8032 . engineered_tag 1554 1578 . + 0 ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071 BBa_I8032 . CDS 792 1514 . + 0 ID=annotation301192;Name=rhlR;Parent=BBa_C0071 BBa_I8032 . CDS 81 740 . + 0 ID=BBa_C0040;Name=BBa_C0040 BBa_I8032 . tag 701 734 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_I8032 . engineered_tag 741 765 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_I8032 . CDS 84 700 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 >BBa_I8032 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatacta gatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggc gtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccct tcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggc cgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgg atgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctca gcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcat gatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgc gaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagca cggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggc gctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctgg cagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagatggtgagcaagggc gaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccg gcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctg gcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttc ttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcg ccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacat cctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaag gtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcg gcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcg cgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgct gcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc ctttctgcgtttata