LOCUS       BBa_I8033               1990 bp    DNA     linear       08-May-2024
DEFINITION  Booperator aiia test 4: effect of tetR on production of RhlR
ACCESSION   BBa_I8033
VERSION     BBa_I8033.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=rhlR
     misc_feature    804..836
                     /label=LVA
     misc_feature    843..867
                     /label=Help:Barcodes
     CDS             81..842
                     /label=BBa_C0071
     stem_loop       887..930
                     /label=stem_loop
     misc_feature    876..955
                     /label=BBa_B0010
     terminator      876..955
                     /label=BBa_B0010
     stem_loop       971..990
                     /label=T7 TE
     misc_feature    997..997
                     /label=stop
     polyA_site      991..1004
                     /label=polya
     misc_feature    964..1004
                     /label=BBa_B0012
     terminator      964..1004
                     /label=BBa_B0012
     misc_feature    1013..1032
                     /label=RhlR
     promoter        1031..1036
                     /label=-35
     promoter        1054..1059
                     /label=-10
     misc_feature    1065..1065
                     /label=start
     promoter        1013..1065
                     /label=BBa_R0071
     misc_feature    1078..1081
                     /label=conserved
     RBS             1074..1085
                     /label=BBa_B0034
     CDS             1092..1853
                     /label=YFP (LVA)
     misc_feature    1848..1853
                     /label=2
     misc_feature    1809..1847
                     /label=SsrA
     misc_feature    1333..1333
                     /label=A (Q->L)
     misc_feature    1092..1853
                     /label=BBa_E0032
     misc_feature    1092..1853
                     /label=BBa_E0032
     stem_loop       1873..1916
                     /label=stem_loop
     misc_feature    1862..1941
                     /label=BBa_B0010
     terminator      1862..1941
                     /label=BBa_B0010
     stem_loop       1957..1976
                     /label=T7 TE
     misc_feature    1983..1983
                     /label=stop
     polyA_site      1977..1990
                     /label=polya
     misc_feature    1950..1990
                     /label=BBa_B0012
     terminator      1950..1990
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt
      121 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg
      181 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca
      241 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga
      301 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg
      361 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt
      421 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc
      481 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc
      541 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc
      601 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg
      661 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact
      721 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct
      781 acgccgcggc gctgggtctc atcgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc
      901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtcctgtga
     1021 aatctggcag ttaccgttag ctttcgaatt ggctaaaaag tgttctacta gagaaagagg
     1081 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1141 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1201 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1261 cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg
     1321 accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1381 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1441 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1501 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1561 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1621 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1681 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1741 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1801 tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga
     1861 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1921 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1981 tgcgtttata
//