LOCUS       BBa_I8034               2697 bp    DNA     linear       09-May-2024
DEFINITION  Booperator aiia test 5: effect of tetR on production of LuxR
ACCESSION   BBa_I8034
VERSION     BBa_I8034.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..700
                     /label=tetR
     misc_feature    701..734
                     /label=SsrA
     misc_feature    741..765
                     /label=Help:Barcodes
     CDS             81..740
                     /label=BBa_C0040
     misc_feature    778..781
                     /label=conserved
     RBS             774..785
                     /label=BBa_B0034
     misc_feature    792..793
                     /label=prefix
     misc_feature    965..965
                     /label=T
     misc_feature    1283..1283
                     /label=A
     CDS             792..1541
                     /label=luxR
     misc_feature    792..1547
                     /label=BBa_C0062
     misc_feature    1548..1572
                     /label=Help:Barcodes
     CDS             792..1547
                     /label=BBa_C0062
     stem_loop       1592..1635
                     /label=stem_loop
     misc_feature    1581..1660
                     /label=BBa_B0010
     terminator      1581..1660
                     /label=BBa_B0010
     stem_loop       1676..1695
                     /label=T7 TE
     misc_feature    1702..1702
                     /label=stop
     polyA_site      1696..1709
                     /label=polya
     misc_feature    1669..1709
                     /label=BBa_B0012
     terminator      1669..1709
                     /label=BBa_B0012
     misc_feature    1718..1737
                     /label=LuxR/HSL
     promoter        1737..1742
                     /label=-35
     promoter        1759..1764
                     /label=-10
     misc_feature    1770..1770
                     /label=start
     misc_feature    1718..1772
                     /label=BBa_R0062
     promoter        1718..1772
                     /label=BBa_R0062
     misc_feature    1785..1788
                     /label=conserved
     RBS             1781..1792
                     /label=BBa_B0034
     CDS             1799..2560
                     /label=YFP (LVA)
     misc_feature    2555..2560
                     /label=2
     misc_feature    2516..2554
                     /label=SsrA
     misc_feature    2040..2040
                     /label=A (Q->L)
     misc_feature    1799..2560
                     /label=BBa_E0032
     misc_feature    1799..2560
                     /label=BBa_E0032
     stem_loop       2580..2623
                     /label=stem_loop
     misc_feature    2569..2648
                     /label=BBa_B0010
     terminator      2569..2648
                     /label=BBa_B0010
     stem_loop       2664..2683
                     /label=T7 TE
     misc_feature    2690..2690
                     /label=stop
     polyA_site      2684..2697
                     /label=polya
     misc_feature    2657..2697
                     /label=BBa_B0012
     terminator      2657..2697
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
      121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg
      781 agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta
      841 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac
      901 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt
      961 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa
     1021 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat
     1081 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag
     1141 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta
     1201 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga
     1261 acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata
     1321 ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag
     1381 gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc
     1441 atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag
     1501 caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct
     1561 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1621 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1681 accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta
     1741 cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat
     1801 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1861 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1921 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1981 cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct
     2041 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2101 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2161 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2221 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2281 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2341 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2401 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2461 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc
     2521 tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa
     2581 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2641 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//