##gff-version 3
##sequence-region BBa_I8034 1 2697
BBa_I8034	.	CDS	81	740	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I8034	.	tag	701	734	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I8034	.	engineered_tag	741	765	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I8034	.	CDS	84	700	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I8034	.	CDS	792	1547	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I8034	.	point_mutation	1283	1283	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I8034	.	engineered_region	792	1547	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I8034	.	engineered_region	792	793	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I8034	.	CDS	792	1541	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I8034	.	point_mutation	965	965	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I8034	.	engineered_tag	1548	1572	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I8034	.	terminator	2657	2697	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8034	.	stem_loop	2664	2683	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8034	.	stop_codon	2690	2690	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8034	.	polyA_site	2684	2697	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8034	.	engineered_region	2657	2697	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8034	.	promoter	1718	1772	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I8034	.	engineered_region	1718	1772	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I8034	.	start_codon	1770	1770	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I8034	.	promoter	1759	1764	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I8034	.	promoter	1737	1742	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I8034	.	non_covalent_binding_site	1718	1737	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I8034	.	terminator	2569	2648	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8034	.	stem_loop	2580	2623	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8034	.	engineered_region	2569	2648	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8034	.	terminator	1669	1709	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8034	.	stem_loop	1676	1695	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8034	.	stop_codon	1702	1702	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8034	.	polyA_site	1696	1709	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8034	.	engineered_region	1669	1709	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8034	.	engineered_region	1799	2560	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I8034	.	sequence_alteration	2040	2040	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I8034	.	engineered_region	1799	2560	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I8034	.	tag	2516	2554	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I8034	.	stop_codon	2555	2560	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I8034	.	CDS	1799	2560	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I8034	.	ribosome_entry_site	774	785	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8034	.	conserved_region	778	781	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8034	.	ribosome_entry_site	1781	1792	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8034	.	conserved_region	1785	1788	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8034	.	terminator	1581	1660	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8034	.	stem_loop	1592	1635	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8034	.	engineered_region	1581	1660	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8034	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I8034	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8034	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8034	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8034	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8034	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8034	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8034	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I8034
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgagg
agctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcga
gggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccc
accctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttca
agtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccga
ggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtga
acttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcga
cggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgat
cacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaa
acgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata