LOCUS       BBa_I8035               1986 bp    DNA     linear       08-May-2024
DEFINITION  Booperator aiia test 6: Effect of tetR on production of LuxR
ACCESSION   BBa_I8035
VERSION     BBa_I8035.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..1849
                     /label=YFP (LVA)
     misc_feature    1844..1849
                     /label=2
     misc_feature    1805..1843
                     /label=SsrA
     misc_feature    1329..1329
                     /label=A (Q->L)
     misc_feature    1088..1849
                     /label=BBa_E0032
     misc_feature    1088..1849
                     /label=BBa_E0032
     stem_loop       1869..1912
                     /label=stem_loop
     misc_feature    1858..1937
                     /label=BBa_B0010
     terminator      1858..1937
                     /label=BBa_B0010
     stem_loop       1953..1972
                     /label=T7 TE
     misc_feature    1979..1979
                     /label=stop
     polyA_site      1973..1986
                     /label=polya
     misc_feature    1946..1986
                     /label=BBa_B0012
     terminator      1946..1986
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1141 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1201 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1261 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca
     1321 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1381 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1441 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1501 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1561 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1621 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1681 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1741 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1801 caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca
     1861 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1921 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1981 tttata
//