##gff-version 3
##sequence-region BBa_I8100 1 2920
BBa_I8100	.	terminator	774	853	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8100	.	stem_loop	785	828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8100	.	engineered_region	774	853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8100	.	CDS	81	740	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I8100	.	tag	701	734	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I8100	.	engineered_tag	741	765	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I8100	.	CDS	84	700	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I8100	.	terminator	862	902	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8100	.	stem_loop	869	888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8100	.	stop_codon	895	895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8100	.	polyA_site	889	902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8100	.	engineered_region	862	902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8100	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I8100	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8100	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8100	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8100	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8100	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8100	.	CDS	2003	2758	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I8100	.	point_mutation	2494	2494	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I8100	.	engineered_region	2003	2758	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I8100	.	engineered_region	2003	2004	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I8100	.	point_mutation	2176	2176	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I8100	.	CDS	2003	2752	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I8100	.	engineered_tag	2759	2783	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I8100	.	terminator	2880	2920	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8100	.	stem_loop	2887	2906	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8100	.	stop_codon	2913	2913	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8100	.	polyA_site	2907	2920	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8100	.	engineered_region	2880	2920	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8100	.	promoter	1923	1976	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I8100	.	engineered_region	1923	1976	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8100	.	promoter	1942	1947	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8100	.	promoter	1965	1970	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8100	.	non_covalent_binding_site	1923	1941	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8100	.	non_covalent_binding_site	1948	1966	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8100	.	terminator	1874	1914	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8100	.	stem_loop	1881	1900	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8100	.	stop_codon	1907	1907	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8100	.	polyA_site	1901	1914	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8100	.	engineered_region	1874	1914	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8100	.	terminator	1786	1865	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8100	.	stem_loop	1797	1840	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8100	.	engineered_region	1786	1865	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8100	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8100	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8100	.	ribosome_entry_site	1985	1996	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8100	.	conserved_region	1989	1992	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8100	.	CDS	991	1752	.	+	0	ID=BBa_C0071;Name=BBa_C0071
BBa_I8100	.	tag	1714	1746	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_I8100	.	CDS	991	1713	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_I8100	.	engineered_tag	1753	1777	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_I8100	.	terminator	2792	2871	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8100	.	stem_loop	2803	2846	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8100	.	engineered_region	2792	2871	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8100	.	promoter	911	964	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I8100	.	engineered_region	911	964	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8100	.	promoter	930	935	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8100	.	promoter	953	958	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8100	.	non_covalent_binding_site	911	929	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8100	.	non_covalent_binding_site	936	954	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8100	.	ribosome_entry_site	973	984	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I8100	.	conserved_region	977	980	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I8100
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtga
tagagatactgagcactactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacg
gtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctggg
cttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatccc
aaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcgg
aaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgt
cggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctcc
agcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagc
atccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagag
ttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttc
gacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctt
tagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagca
ctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaag
cttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgc
gatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattat
gatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatat
ttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttag
tttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagt
ttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaa
ataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttggga
tatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaaca
aaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgat
agtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata