LOCUS BBa_I8101 2920 bp DNA linear 09-May-2024 DEFINITION RhlR and LasR constitutive generation (using TetR) ACCESSION BBa_I8101 VERSION BBa_I8101.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..700 /label=tetR misc_feature 701..734 /label=SsrA misc_feature 741..765 /label=Help:Barcodes CDS 81..740 /label=BBa_C0040 stem_loop 785..828 /label=stem_loop misc_feature 774..853 /label=BBa_B0010 terminator 774..853 /label=BBa_B0010 stem_loop 869..888 /label=T7 TE misc_feature 895..895 /label=stop polyA_site 889..902 /label=polya misc_feature 862..902 /label=BBa_B0012 terminator 862..902 /label=BBa_B0012 misc_feature 911..929 /label=TetR 1 misc_feature 911..964 /label=BBa_R0040 promoter 930..935 /label=-35 misc_feature 936..954 /label=TetR 2 promoter 953..958 /label=-10 promoter 911..964 /label=BBa_R0040 misc_feature 977..980 /label=conserved RBS 973..984 /label=BBa_B0034 CDS 991..1713 /label=rhlR misc_feature 1714..1746 /label=LVA misc_feature 1753..1777 /label=Help:Barcodes CDS 991..1752 /label=BBa_C0071 stem_loop 1797..1840 /label=stem_loop misc_feature 1786..1865 /label=BBa_B0010 terminator 1786..1865 /label=BBa_B0010 stem_loop 1881..1900 /label=T7 TE misc_feature 1907..1907 /label=stop polyA_site 1901..1914 /label=polya misc_feature 1874..1914 /label=BBa_B0012 terminator 1874..1914 /label=BBa_B0012 misc_feature 1923..1941 /label=TetR 1 misc_feature 1923..1976 /label=BBa_R0040 promoter 1942..1947 /label=-35 misc_feature 1948..1966 /label=TetR 2 promoter 1965..1970 /label=-10 promoter 1923..1976 /label=BBa_R0040 misc_feature 1989..1992 /label=conserved RBS 1985..1996 /label=BBa_B0034 CDS 2003..2719 /label=lasR misc_feature 2720..2752 /label=LVA misc_feature 2482..2482 /label=G misc_feature 2759..2783 /label=Help:Barcodes CDS 2003..2758 /label=BBa_C0079 stem_loop 2803..2846 /label=stem_loop misc_feature 2792..2871 /label=BBa_B0010 terminator 2792..2871 /label=BBa_B0010 stem_loop 2887..2906 /label=T7 TE misc_feature 2913..2913 /label=stop polyA_site 2907..2920 /label=polya misc_feature 2880..2920 /label=BBa_B0012 terminator 2880..2920 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat 121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca 781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 901 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga 961 gcactactag agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg 1021 tgggacggtt tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc 1081 ctggaaaagg aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg 1141 attcccttca cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag 1201 cgataccaga tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc 1261 tcggaaatgg tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag 1321 gctcgcgatt ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg 1381 ctcagcgtgc tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa 1441 atccgcctgc ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag 1501 catccgatgc tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa 1561 tggaccgccg acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc 1621 acggtgaact tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg 1681 gctgccgcct acgccgcggc gctgggtctc atcgctgcaa acgacgaaaa ctacgcttta 1741 gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa 1801 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1861 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 1921 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact 1981 agagaaagag gagaaatact agatggcctt ggttgacggt tttcttgagc tggaacgctc 2041 aagtggaaaa ttggagtgga gcgccatcct ccagaagatg gcgagcgacc ttggattctc 2101 gaagatcctg ttcggcctgt tgcctaagga cagccaggac tacgagaacg ccttcatcgt 2161 cggcaactac ccggccgcct ggcgcgagca ttacgaccgg gctggctacg cgcgggtcga 2221 cccgacggtc agtcactgta cccagagcgt actgccgatt ttctgggaac cgtccatcta 2281 ccagacgcga aagcagcacg agttcttcga ggaagcctcg gccgccggcc tggtgtatgg 2341 gctgaccatg ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc tcagcgtgga 2401 agcggaaaac cgggccgagg ccaaccgttt catagagtcg gtcctgccga ccctgtggat 2461 gctcaaggac tacgcactgc aaagcggtgc cggactggcc ttcgaacatc cggtcagcaa 2521 accggtggtt ctgaccagcc gggagaagga agtgttgcag tggtgcgcca tcggcaagac 2581 cagttgggag atatcggtta tctgcaactg ctcggaagcc aatgtgaact tccatatggg 2641 aaatattcgg cggaagttcg gtgtgacctc ccgccgcgta gcggccatta tggccgttaa 2701 tttgggtctt attactctcg ctgcaaacga cgaaaactac gctttagtag cttaataaca 2761 ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag 2821 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 2881 cacactggct caccttcggg tgggcctttc tgcgtttata //