BBa_I8101
type: ComponentDefinition
persistent identity: BBa I8101
display id: BBa_I8101
version: 1
was generated by: iGEM to SBOL conversion
description: RhlR and LasR constitutive generation (using TetR)
created: 2004-01-30T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
mutable description: Produces RhlR and LasR constitutively under control of a TetR self-repressor.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Ronny Krashinsky
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0071
component: BBa_B0034
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_C0040
component: BBa_C0079
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0071
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_I8101_sequence/1

BBa_C0071
type: Component
persistent identity: component980894
display id: component980894
version: 1
top level: BBa_I8101
definition: rhlR
access: public

BBa_B0034
type: Component
persistent identity: component980884
display id: component980884
version: 1
top level: BBa_I8101
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component980876
display id: component980876
version: 1
top level: BBa_I8101
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component980861
display id: component980861
version: 1
top level: BBa_I8101
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component980851
display id: component980851
version: 1
top level: BBa_I8101
definition: BBa_B0010
access: public

BBa_C0040
type: Component
persistent identity: component980845
display id: component980845
version: 1
top level: BBa_I8101
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component980835
display id: component980835
version: 1
top level: BBa_I8101
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component980827
display id: component980827
version: 1
top level: BBa_I8101
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component980962
display id: component980962
version: 1
top level: BBa_I8101
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component980952
display id: component980952
version: 1
top level: BBa_I8101
definition: BBa_B0010
access: public

BBa_C0079
type: Component
persistent identity: component980946
display id: component980946
version: 1
top level: BBa_I8101
definition: lasr
access: public

BBa_B0034
type: Component
persistent identity: component980933
display id: component980933
version: 1
top level: BBa_I8101
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component980925
display id: component980925
version: 1
top level: BBa_I8101
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component980910
display id: component980910
version: 1
top level: BBa_I8101
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component980900
display id: component980900
version: 1
top level: BBa_I8101
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation980827
display id: annotation980827
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980827/range980827/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I8101/annotation980827/range980827/1
type: Range
persistent identity: range980827
display id: range980827
version: 1
top level: BBa_I8101
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980835
display id: annotation980835
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980835/range980835/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I8101/annotation980835/range980835/1
type: Range
persistent identity: range980835
display id: range980835
version: 1
top level: BBa_I8101
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation980845
display id: annotation980845
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980845/range980845/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I8101/annotation980845/range980845/1
type: Range
persistent identity: range980845
display id: range980845
version: 1
top level: BBa_I8101
direction: direction/forward
start: 81
end: 740
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980851
display id: annotation980851
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980851/range980851/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I8101/annotation980851/range980851/1
type: Range
persistent identity: range980851
display id: range980851
version: 1
top level: BBa_I8101
direction: direction/forward
start: 774
end: 853
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980861
display id: annotation980861
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980861/range980861/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I8101/annotation980861/range980861/1
type: Range
persistent identity: range980861
display id: range980861
version: 1
top level: BBa_I8101
direction: direction/forward
start: 862
end: 902
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation980876
display id: annotation980876
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980876/range980876/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I8101/annotation980876/range980876/1
type: Range
persistent identity: range980876
display id: range980876
version: 1
top level: BBa_I8101
direction: direction/forward
start: 911
end: 964
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980884
display id: annotation980884
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980884/range980884/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I8101/annotation980884/range980884/1
type: Range
persistent identity: range980884
display id: range980884
version: 1
top level: BBa_I8101
direction: direction/forward
start: 973
end: 984
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation980894
display id: annotation980894
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980894/range980894/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_I8101/annotation980894/range980894/1
type: Range
persistent identity: range980894
display id: range980894
version: 1
top level: BBa_I8101
direction: direction/forward
start: 991
end: 1752
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980900
display id: annotation980900
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980900/range980900/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I8101/annotation980900/range980900/1
type: Range
persistent identity: range980900
display id: range980900
version: 1
top level: BBa_I8101
direction: direction/forward
start: 1786
end: 1865
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980910
display id: annotation980910
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980910/range980910/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I8101/annotation980910/range980910/1
type: Range
persistent identity: range980910
display id: range980910
version: 1
top level: BBa_I8101
direction: direction/forward
start: 1874
end: 1914
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation980925
display id: annotation980925
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980925/range980925/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I8101/annotation980925/range980925/1
type: Range
persistent identity: range980925
display id: range980925
version: 1
top level: BBa_I8101
direction: direction/forward
start: 1923
end: 1976
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation980933
display id: annotation980933
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980933/range980933/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I8101/annotation980933/range980933/1
type: Range
persistent identity: range980933
display id: range980933
version: 1
top level: BBa_I8101
direction: direction/forward
start: 1985
end: 1996
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation980946
display id: annotation980946
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980946/range980946/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_I8101/annotation980946/range980946/1
type: Range
persistent identity: range980946
display id: range980946
version: 1
top level: BBa_I8101
direction: direction/forward
start: 2003
end: 2758
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation980952
display id: annotation980952
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980952/range980952/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I8101/annotation980952/range980952/1
type: Range
persistent identity: range980952
display id: range980952
version: 1
top level: BBa_I8101
direction: direction/forward
start: 2792
end: 2871
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation980962
display id: annotation980962
version: 1
top level: BBa_I8101
location: https://synbiohub.org/public/igem/BBa_I8101/annotation980962/range980962/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I8101/annotation980962/range980962/1
type: Range
persistent identity: range980962
display id: range980962
version: 1
top level: BBa_I8101
direction: direction/forward
start: 2880
end: 2920
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutated 480 from g to a to remove PstI site
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lasR
sequence annotation: G
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
top level: lasr
direction: direction/forward
start: 1
end: 717
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
top level: lasr
direction: direction/forward
start: 480
end: 480
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
top level: lasr
direction: direction/forward
start: 718
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
top level: lasr
direction: direction/forward
start: 757
end: 781
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: rhlR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
top level: rhlR
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
top level: rhlR
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
top level: rhlR
direction: direction/forward
start: 763
end: 787
orientation: inline

https://synbiohub.org/public/igem/BBa_I8101_sequence/1
type: Sequence
persistent identity: BBa I8101 sequence
display id: BBa_I8101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0079_sequence/1
type: Sequence
persistent identity: BBa C0079 sequence
display id: BBa_C0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0071_sequence/1
type: Sequence
persistent identity: BBa C0071 sequence
display id: BBa_C0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z