LOCUS BBa_I8500 3250 bp DNA linear 25-Nov-2024 DEFINITION 3OC6HSL->Inverter->lacZalpha ACCESSION BBa_I8500 VERSION BBa_I8500.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 RBS 1070..1084 /label=RBS-1\Strong RBS 1077..1081 /label=RBS misc_feature 1070..1084 /label=BBa_B0030 RBS 1070..1084 /label=BBa_B0030 CDS 1091..2518 /label=BBa_C2006 stem_loop 2538..2581 /label=stem_loop misc_feature 2527..2606 /label=BBa_B0010 terminator 2527..2606 /label=BBa_B0010 stem_loop 2622..2641 /label=T7 TE misc_feature 2648..2648 /label=stop polyA_site 2642..2655 /label=polya misc_feature 2615..2655 /label=BBa_B0012 terminator 2615..2655 /label=BBa_B0012 promoter 2673..2678 /label=-35 promoter 2696..2701 /label=-10 misc_feature 2683..2696 /label=Zif23 misc_feature 2664..2672 /label=consensus misc_feature 2708..2708 /label=(+1) promoter 2664..2708 /label=BBa_R2000 misc_feature 1070..2708 /label=BBa_Q20060 misc_feature 1..2708 /label=BBa_P20060 misc_feature 2721..2724 /label=conserved RBS 2717..2728 /label=BBa_B0034 misc_feature 2735..2749 /label=lacZ gene fragment CDS 2735..3088 /label=lacZ alpha promoter 2760..2769 /label=T3 promoter promoter 2907..2925 /label=T7 promoter misc_feature 2812..2812 /label=C misc_feature 2815..2815 /label=T misc_feature 2821..2821 /label=A misc_feature 2842..2842 /label=C misc_feature 2845..2845 /label=G misc_feature 2933..3082 /label=lacZ gene fragment misc_feature 3082..3082 /label=inserted T misc_feature 3089..3113 /label=barcode misc_feature 2735..3113 /label=BBa_E0035 stem_loop 3133..3176 /label=stem_loop misc_feature 3122..3201 /label=BBa_B0010 terminator 3122..3201 /label=BBa_B0010 stem_loop 3217..3236 /label=T7 TE misc_feature 3243..3243 /label=stop polyA_site 3237..3250 /label=polya misc_feature 3210..3250 /label=BBa_B0012 terminator 3210..3250 /label=BBa_B0012 terminator 3122..3250 /label=BBa_B0015 misc_feature 2717..3250 /label=BBa_E0435 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga 1081 gaaatactag atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg 1141 ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac 1201 cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg 1261 ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt 1321 ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc 1381 cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat 1441 ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga 1501 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt 1561 cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta 1621 cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt 1681 tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc 1741 ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga 1801 caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa 1861 accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc 1921 aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga 1981 caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg 2041 tattgccgcc actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat 2101 gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac 2161 tgtcgatgaa gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa 2221 caataacaac aacctcggga tcgagggaag gatgaaaccg ttccagtgcc gtatctgcat 2281 gcgtaacttc tcccgttccg accacctgac cacccacatc cgtacccaca ccggtgaaaa 2341 accgttcgct tgcgacatct gcggtcgtaa attcgctcgt tccgacgaac gtaaacgtca 2401 ccgtaaattg cagcacatga aacagctgga agacaaagtt gaagaactgc tgtccaaaaa 2461 ctaccacctg gaaaacgaag ttgctcgtct gaaaaaactg gttggtgaac gttaataata 2521 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2581 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2641 ctttctgcgt ttatatacta gagagtttat tcttgacatg gtcccacgcg cgtgggatac 2701 tacgtcagta ctagagaaag aggagaaata ctagatgacc atgattacgc caagcgcgca 2761 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg cagcactaga 2821 gctagtggat cccccgggct gtagaaattc gatatcaagc ttatcgatac cgtcgacctc 2881 gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 2941 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 3001 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 3061 ccaacagttg cgcagcctga attaataacg ctgatagtgc tagtgtagat cgctactaga 3121 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 3181 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 3241 tgcgtttata //