##gff-version 3
##sequence-region BBa_I8500 1 3250
BBa_I8500	.	engineered_region	1	2708	.	+	0	ID=BBa_P20060;Name=BBa_P20060
BBa_I8500	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_P20060
BBa_I8500	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_I8500	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8500	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_I8500	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8500	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8500	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8500	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8500	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8500	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_I8500	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I8500	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8500	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8500	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8500	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8500	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I8500	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8500	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8500	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_I8500	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I8500	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I8500	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I8500	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I8500	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I8500	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_I8500	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I8500	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I8500	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I8500	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I8500	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I8500	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I8500	.	engineered_region	1070	2708	.	+	0	ID=BBa_Q20060;Name=BBa_Q20060;Parent=BBa_P20060
BBa_I8500	.	CDS	1091	2518	.	+	0	ID=BBa_C2006;Name=BBa_C2006;Parent=BBa_Q20060
BBa_I8500	.	terminator	2527	2606	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q20060
BBa_I8500	.	engineered_region	2527	2606	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8500	.	stem_loop	2538	2581	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8500	.	terminator	2615	2655	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q20060
BBa_I8500	.	engineered_region	2615	2655	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8500	.	stem_loop	2622	2641	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8500	.	stop_codon	2648	2648	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8500	.	polyA_site	2642	2655	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8500	.	promoter	2664	2708	.	+	0	ID=BBa_R2000;Name=BBa_R2000;Parent=BBa_Q20060
BBa_I8500	.	sequence_feature	2664	2672	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_I8500	.	promoter	2673	2678	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_I8500	.	non_covalent_binding_site	2683	2696	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_I8500	.	promoter	2696	2701	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_I8500	.	start_codon	2708	2708	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_I8500	.	ribosome_entry_site	1070	1084	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_Q20060
BBa_I8500	.	ribosome_entry_site	1077	1081	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I8500	.	engineered_region	1070	1084	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I8500	.	ribosome_entry_site	1070	1084	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I8500	.	engineered_region	2717	3250	.	+	0	ID=BBa_E0435;Name=BBa_E0435
BBa_I8500	.	terminator	3122	3250	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_I8500	.	terminator	3210	3250	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I8500	.	engineered_region	3210	3250	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8500	.	stem_loop	3217	3236	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8500	.	stop_codon	3243	3243	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8500	.	polyA_site	3237	3250	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8500	.	terminator	3122	3201	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I8500	.	engineered_region	3122	3201	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8500	.	stem_loop	3133	3176	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8500	.	ribosome_entry_site	2717	2728	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_I8500	.	conserved_region	2721	2724	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8500	.	engineered_region	2735	3113	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_I8500	.	sequence_feature	2933	3082	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_I8500	.	promoter	2907	2925	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_I8500	.	CDS	2735	3088	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_I8500	.	sequence_alteration	3082	3082	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_I8500	.	sequence_alteration	2821	2821	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_I8500	.	engineered_tag	3089	3113	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_I8500	.	sequence_alteration	2845	2845	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_I8500	.	promoter	2760	2769	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_I8500	.	sequence_alteration	2812	2812	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_I8500	.	sequence_alteration	2842	2842	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_I8500	.	sequence_alteration	2815	2815	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_I8500	.	sequence_feature	2735	2749	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
>BBa_I8500
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgaaaatcgaagaag
gtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagatac
cggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccct
gacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaag
cgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatcgctgt
tgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgctggat
aaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgattgctg
ctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaa
agcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcagaagct
gcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagtgaatt
atggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgc
cgccagtccgaacaaagagctggcaaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcggttaat
aaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatccacgtattgccgccacta
tggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggt
gatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactaattcgagctcgaacaacaac
aacaataacaataacaacaacctcgggatcgagggaaggatgaaaccgttccagtgccgtatctgcatgcgtaacttct
cccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaa
attcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactg
ctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgttaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagtttattcttgacatggtccca
cgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaa
ccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtag
aaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtat
tacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcag
cacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaa
ttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata