LOCUS BBa_I8501 5946 bp DNA linear 16-Feb-2025 DEFINITION 3OC6HSL->Inverter->lacZ ACCESSION BBa_I8501 VERSION BBa_I8501.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 RBS 1070..1084 /label=RBS-1\Strong RBS 1077..1081 /label=RBS misc_feature 1070..1084 /label=BBa_B0030 RBS 1070..1084 /label=BBa_B0030 CDS 1091..2518 /label=BBa_C2006 stem_loop 2538..2581 /label=stem_loop misc_feature 2527..2606 /label=BBa_B0010 terminator 2527..2606 /label=BBa_B0010 stem_loop 2622..2641 /label=T7 TE misc_feature 2648..2648 /label=stop polyA_site 2642..2655 /label=polya misc_feature 2615..2655 /label=BBa_B0012 terminator 2615..2655 /label=BBa_B0012 promoter 2673..2678 /label=-35 promoter 2696..2701 /label=-10 misc_feature 2683..2696 /label=Zif23 misc_feature 2664..2672 /label=consensus misc_feature 2708..2708 /label=(+1) promoter 2664..2708 /label=BBa_R2000 misc_feature 1070..2708 /label=BBa_Q20060 misc_feature 1..2708 /label=BBa_P20060 misc_feature 2721..2724 /label=conserved RBS 2717..2728 /label=BBa_B0034 misc_feature 5758..5758 /label=C->T CDS 2735..5809 /label=lacZ misc_feature 2735..2737 /label=START misc_feature 5807..5809 /label=STOP CDS 2735..5809 /label=BBa_I732005 stem_loop 5829..5872 /label=stem_loop misc_feature 5818..5897 /label=BBa_B0010 terminator 5818..5897 /label=BBa_B0010 stem_loop 5913..5932 /label=T7 TE misc_feature 5939..5939 /label=stop polyA_site 5933..5946 /label=polya misc_feature 5906..5946 /label=BBa_B0012 terminator 5906..5946 /label=BBa_B0012 misc_feature 2717..5946 /label=BBa_I732019 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga 1081 gaaatactag atgaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg 1141 ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac 1201 cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg 1261 ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt 1321 ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc 1381 cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat 1441 ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga 1501 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt 1561 cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta 1621 cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt 1681 tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc 1741 ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga 1801 caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa 1861 accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc 1921 aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga 1981 caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg 2041 tattgccgcc actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat 2101 gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac 2161 tgtcgatgaa gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa 2221 caataacaac aacctcggga tcgagggaag gatgaaaccg ttccagtgcc gtatctgcat 2281 gcgtaacttc tcccgttccg accacctgac cacccacatc cgtacccaca ccggtgaaaa 2341 accgttcgct tgcgacatct gcggtcgtaa attcgctcgt tccgacgaac gtaaacgtca 2401 ccgtaaattg cagcacatga aacagctgga agacaaagtt gaagaactgc tgtccaaaaa 2461 ctaccacctg gaaaacgaag ttgctcgtct gaaaaaactg gttggtgaac gttaataata 2521 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2581 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2641 ctttctgcgt ttatatacta gagagtttat tcttgacatg gtcccacgcg cgtgggatac 2701 tacgtcagta ctagagaaag aggagaaata ctagatgacc atgattacgg attcactggc 2761 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 2821 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 2881 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc 2941 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc 3001 aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac 3061 ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa 3121 tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc 3181 ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc 3241 gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt 3301 gctgcgctgg agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat 3361 tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc 3421 cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg 3481 cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc 3541 cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg 3601 cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct 3661 ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg 3721 cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc 3781 gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga 3841 tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg 3901 ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta 3961 tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga 4021 tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg 4081 taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca 4141 cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg 4201 cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga 4261 agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg 4321 agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg 4381 tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg 4441 ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta 4501 cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt 4561 tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca 4621 gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga 4681 taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt 4741 gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc 4801 ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg 4861 gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt 4921 gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg 4981 catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat 5041 gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc 5101 gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga 5161 acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga 5221 tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac 5281 cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt 5341 tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct 5401 ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga 5461 ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc 5521 gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc 5581 acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat 5641 ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg 5701 tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaatttca 5761 gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataat actagagcca 5821 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 5881 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 5941 tttata //