LOCUS BBa_I8510 4198 bp DNA linear 12-May-2024 DEFINITION 3OC6HSL->Inverter->lacZalpha with orthogonal GFP protein generator ACCESSION BBa_I8510 VERSION BBa_I8510.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 CDS 84..803 /label=GFP protein CDS 84..803 /label=BBa_E0040 stem_loop 823..866 /label=stem_loop misc_feature 812..891 /label=BBa_B0010 terminator 812..891 /label=BBa_B0010 stem_loop 907..926 /label=T7 TE misc_feature 933..933 /label=stop polyA_site 927..940 /label=polya misc_feature 900..940 /label=BBa_B0012 terminator 900..940 /label=BBa_B0012 misc_feature 1..940 /label=BBa_I7100 misc_feature 949..967 /label=TetR 1 misc_feature 949..1002 /label=BBa_R0040 promoter 968..973 /label=-35 misc_feature 974..992 /label=TetR 2 promoter 991..996 /label=-10 promoter 949..1002 /label=BBa_R0040 misc_feature 1015..1018 /label=conserved RBS 1011..1022 /label=BBa_B0034 misc_feature 1029..1030 /label=prefix misc_feature 1202..1202 /label=T misc_feature 1520..1520 /label=A CDS 1029..1778 /label=luxR misc_feature 1029..1784 /label=BBa_C0062 misc_feature 1785..1809 /label=Help:Barcodes CDS 1029..1784 /label=BBa_C0062 stem_loop 1829..1872 /label=stem_loop misc_feature 1818..1897 /label=BBa_B0010 terminator 1818..1897 /label=BBa_B0010 stem_loop 1913..1932 /label=T7 TE misc_feature 1939..1939 /label=stop polyA_site 1933..1946 /label=polya misc_feature 1906..1946 /label=BBa_B0012 terminator 1906..1946 /label=BBa_B0012 terminator 1818..1946 /label=BBa_B0015 misc_feature 1955..1974 /label=LuxR/HSL promoter 1974..1979 /label=-35 promoter 1996..2001 /label=-10 misc_feature 2007..2007 /label=start misc_feature 1955..2009 /label=BBa_R0062 promoter 1955..2009 /label=BBa_R0062 misc_feature 949..2009 /label=BBa_F2620 RBS 2018..2032 /label=RBS-1\Strong RBS 2025..2029 /label=RBS misc_feature 2018..2032 /label=BBa_B0030 RBS 2018..2032 /label=BBa_B0030 CDS 2039..3466 /label=BBa_C2006 stem_loop 3486..3529 /label=stem_loop misc_feature 3475..3554 /label=BBa_B0010 terminator 3475..3554 /label=BBa_B0010 stem_loop 3570..3589 /label=T7 TE misc_feature 3596..3596 /label=stop polyA_site 3590..3603 /label=polya misc_feature 3563..3603 /label=BBa_B0012 terminator 3563..3603 /label=BBa_B0012 promoter 3621..3626 /label=-35 promoter 3644..3649 /label=-10 misc_feature 3631..3644 /label=Zif23 misc_feature 3612..3620 /label=consensus misc_feature 3656..3656 /label=(+1) promoter 3612..3656 /label=BBa_R2000 misc_feature 2018..3656 /label=BBa_Q20060 misc_feature 949..3656 /label=BBa_P20060 misc_feature 3669..3672 /label=conserved RBS 3665..3676 /label=BBa_B0034 misc_feature 3683..3697 /label=lacZ gene fragment CDS 3683..4036 /label=lacZ alpha promoter 3708..3717 /label=T3 promoter promoter 3855..3873 /label=T7 promoter misc_feature 3760..3760 /label=C misc_feature 3763..3763 /label=T misc_feature 3769..3769 /label=A misc_feature 3790..3790 /label=C misc_feature 3793..3793 /label=G misc_feature 3881..4030 /label=lacZ gene fragment misc_feature 4030..4030 /label=inserted T misc_feature 4037..4061 /label=barcode misc_feature 3683..4061 /label=BBa_E0035 stem_loop 4081..4124 /label=stem_loop misc_feature 4070..4149 /label=BBa_B0010 terminator 4070..4149 /label=BBa_B0010 stem_loop 4165..4184 /label=T7 TE misc_feature 4191..4191 /label=stop polyA_site 4185..4198 /label=polya misc_feature 4158..4198 /label=BBa_B0012 terminator 4158..4198 /label=BBa_B0012 terminator 4070..4198 /label=BBa_B0015 misc_feature 3665..4198 /label=BBa_E0435 misc_feature 949..4198 /label=BBa_I8500 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc 121 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg 181 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac 241 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga 301 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg 361 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca 421 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag 481 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca 541 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 781 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag 841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc cctatcagtg 961 atagagattg acatccctat cagtgataga gatactgagc actactagag aaagaggaga 1021 aatactagat gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag 1081 cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt 1141 gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa 1201 tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat 1261 atgatcctat agtagattat tctaactcca atcattcacc aattaattgg aatatatttg 1321 aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc 1381 ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt 1441 ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca 1501 taccattaat tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata 1561 aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa 1621 aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt 1681 taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa 1741 ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga tagtgctagt 1801 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1921 ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac aggtttacgc 1981 aagaaaatgg tttgttatag tcgaataaat actagagatt aaagaggaga aatactagat 2041 gaaaatcgaa gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct 2101 cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc 2161 ggataaactg gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat 2221 cttctgggca cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac 2281 cccggacaaa gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa 2341 cggcaagctg attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga 2401 tctgctgccg aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa 2461 agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct 2521 gattgctgct gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga 2581 cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa 2641 aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg 2701 cgaaacagcg atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt 2761 gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg 2821 cgtgctgagc gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct 2881 cgaaaactat ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg 2941 tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac 3001 tatggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg 3061 gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc 3121 cctgaaagac gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa 3181 cctcgggatc gagggaagga tgaaaccgtt ccagtgccgt atctgcatgc gtaacttctc 3241 ccgttccgac cacctgacca cccacatccg tacccacacc ggtgaaaaac cgttcgcttg 3301 cgacatctgc ggtcgtaaat tcgctcgttc cgacgaacgt aaacgtcacc gtaaattgca 3361 gcacatgaaa cagctggaag acaaagttga agaactgctg tccaaaaact accacctgga 3421 aaacgaagtt gctcgtctga aaaaactggt tggtgaacgt taataatact agagccaggc 3481 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3541 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3601 atatactaga gagtttattc ttgacatggt cccacgcgcg tgggatacta cgtcagtact 3661 agagaaagag gagaaatact agatgaccat gattacgcca agcgcgcaat taaccctcac 3721 taaagggaac aaaagctgga gctccaccgc ggtggcggca gcactagagc tagtggatcc 3781 cccgggctgt agaaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc 3841 ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 3901 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 3961 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 4021 cagcctgaat taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa 4081 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 4141 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //