##gff-version 3
##sequence-region BBa_I8510 1 4198
BBa_I8510	.	engineered_region	949	4198	.	+	0	ID=BBa_I8500;Name=BBa_I8500
BBa_I8510	.	engineered_region	3665	4198	.	+	0	ID=BBa_E0435;Name=BBa_E0435;Parent=BBa_I8500
BBa_I8510	.	ribosome_entry_site	3665	3676	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_I8510	.	conserved_region	3669	3672	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8510	.	terminator	4070	4198	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_I8510	.	terminator	4158	4198	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I8510	.	polyA_site	4185	4198	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8510	.	stem_loop	4165	4184	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8510	.	stop_codon	4191	4191	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8510	.	engineered_region	4158	4198	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8510	.	terminator	4070	4149	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I8510	.	stem_loop	4081	4124	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8510	.	engineered_region	4070	4149	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8510	.	engineered_region	3683	4061	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_I8510	.	CDS	3683	4036	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_I8510	.	sequence_alteration	3763	3763	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_I8510	.	promoter	3855	3873	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_I8510	.	promoter	3708	3717	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_I8510	.	sequence_alteration	3790	3790	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_I8510	.	sequence_alteration	4030	4030	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_I8510	.	sequence_feature	3683	3697	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_I8510	.	sequence_alteration	3793	3793	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_I8510	.	sequence_alteration	3760	3760	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_I8510	.	engineered_tag	4037	4061	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_I8510	.	sequence_feature	3881	4030	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_I8510	.	sequence_alteration	3769	3769	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_I8510	.	engineered_region	949	3656	.	+	0	ID=BBa_P20060;Name=BBa_P20060;Parent=BBa_I8500
BBa_I8510	.	engineered_region	949	2009	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_P20060
BBa_I8510	.	ribosome_entry_site	1011	1022	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_I8510	.	conserved_region	1015	1018	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8510	.	CDS	1029	1784	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_I8510	.	CDS	1029	1778	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I8510	.	point_mutation	1520	1520	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I8510	.	engineered_tag	1785	1809	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I8510	.	point_mutation	1202	1202	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I8510	.	engineered_region	1029	1030	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I8510	.	engineered_region	1029	1784	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I8510	.	terminator	1818	1946	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_I8510	.	terminator	1906	1946	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I8510	.	polyA_site	1933	1946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8510	.	stem_loop	1913	1932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8510	.	stop_codon	1939	1939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8510	.	engineered_region	1906	1946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8510	.	terminator	1818	1897	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I8510	.	stem_loop	1829	1872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8510	.	engineered_region	1818	1897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8510	.	promoter	949	1002	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_I8510	.	non_covalent_binding_site	949	967	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8510	.	non_covalent_binding_site	974	992	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8510	.	promoter	968	973	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8510	.	engineered_region	949	1002	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8510	.	promoter	991	996	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8510	.	promoter	1955	2009	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_I8510	.	start_codon	2007	2007	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I8510	.	promoter	1974	1979	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I8510	.	promoter	1996	2001	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I8510	.	engineered_region	1955	2009	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I8510	.	non_covalent_binding_site	1955	1974	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I8510	.	engineered_region	2018	3656	.	+	0	ID=BBa_Q20060;Name=BBa_Q20060;Parent=BBa_P20060
BBa_I8510	.	terminator	3475	3554	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q20060
BBa_I8510	.	stem_loop	3486	3529	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8510	.	engineered_region	3475	3554	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8510	.	promoter	3612	3656	.	+	0	ID=BBa_R2000;Name=BBa_R2000;Parent=BBa_Q20060
BBa_I8510	.	non_covalent_binding_site	3631	3644	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_I8510	.	sequence_feature	3612	3620	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_I8510	.	promoter	3621	3626	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_I8510	.	promoter	3644	3649	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_I8510	.	start_codon	3656	3656	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_I8510	.	CDS	2039	3466	.	+	0	ID=BBa_C2006;Name=BBa_C2006;Parent=BBa_Q20060
BBa_I8510	.	ribosome_entry_site	2018	2032	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_Q20060
BBa_I8510	.	engineered_region	2018	2032	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I8510	.	ribosome_entry_site	2025	2029	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I8510	.	ribosome_entry_site	2018	2032	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I8510	.	terminator	3563	3603	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q20060
BBa_I8510	.	polyA_site	3590	3603	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8510	.	stem_loop	3570	3589	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8510	.	stop_codon	3596	3596	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8510	.	engineered_region	3563	3603	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8510	.	engineered_region	1	940	.	+	0	ID=BBa_I7100;Name=BBa_I7100
BBa_I8510	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_I7100
BBa_I8510	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I8510	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I8510	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I8510	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I7100
BBa_I8510	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8510	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8510	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8510	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8510	.	terminator	812	891	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I7100
BBa_I8510	.	stem_loop	823	866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8510	.	engineered_region	812	891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8510	.	CDS	84	803	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I7100
BBa_I8510	.	CDS	84	803	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I8510	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I7100
BBa_I8510	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8510	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8510	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8510	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8510	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_I8510
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcac
aaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaa
aactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatat
gaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggg
aactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttta
aagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaaca
aaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaa
caaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaag
atcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaact
atacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgaaaatcgaagaag
gtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagatac
cggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccct
gacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaag
cgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatcgctgt
tgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgctggat
aaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgattgctg
ctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaa
agcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcagaagct
gcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagtgaatt
atggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgc
cgccagtccgaacaaagagctggcaaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcggttaat
aaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatccacgtattgccgccacta
tggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggt
gatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactaattcgagctcgaacaacaac
aacaataacaataacaacaacctcgggatcgagggaaggatgaaaccgttccagtgccgtatctgcatgcgtaacttct
cccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaa
attcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactg
ctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgttaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagtttattcttgacatggtccca
cgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaa
ccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtag
aaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtat
tacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcag
cacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaa
ttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata