##gff-version 3
##sequence-region BBa_I8520 1 3594
BBa_I8520	.	engineered_region	2717	3594	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_I8520	.	CDS	2738	3457	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_I8520	.	CDS	2738	3457	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I8520	.	terminator	3554	3594	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_I8520	.	stem_loop	3561	3580	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8520	.	stop_codon	3587	3587	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8520	.	polyA_site	3581	3594	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8520	.	engineered_region	3554	3594	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8520	.	ribosome_entry_site	2717	2731	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_I8520	.	ribosome_entry_site	2724	2728	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I8520	.	engineered_region	2717	2731	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I8520	.	ribosome_entry_site	2717	2731	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I8520	.	terminator	3466	3545	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_I8520	.	stem_loop	3477	3520	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8520	.	engineered_region	3466	3545	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8520	.	engineered_region	1	2708	.	+	0	ID=BBa_P20060;Name=BBa_P20060
BBa_I8520	.	engineered_region	1070	2708	.	+	0	ID=BBa_Q20060;Name=BBa_Q20060;Parent=BBa_P20060
BBa_I8520	.	CDS	1091	2518	.	+	0	ID=BBa_C2006;Name=BBa_C2006;Parent=BBa_Q20060
BBa_I8520	.	terminator	2527	2606	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q20060
BBa_I8520	.	stem_loop	2538	2581	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8520	.	engineered_region	2527	2606	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8520	.	ribosome_entry_site	1070	1084	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_Q20060
BBa_I8520	.	ribosome_entry_site	1077	1081	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I8520	.	engineered_region	1070	1084	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I8520	.	ribosome_entry_site	1070	1084	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I8520	.	promoter	2664	2708	.	+	0	ID=BBa_R2000;Name=BBa_R2000;Parent=BBa_Q20060
BBa_I8520	.	sequence_feature	2664	2672	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_I8520	.	promoter	2673	2678	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_I8520	.	promoter	2696	2701	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_I8520	.	start_codon	2708	2708	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_I8520	.	non_covalent_binding_site	2683	2696	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_I8520	.	terminator	2615	2655	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q20060
BBa_I8520	.	stem_loop	2622	2641	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8520	.	stop_codon	2648	2648	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8520	.	polyA_site	2642	2655	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8520	.	engineered_region	2615	2655	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8520	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_P20060
BBa_I8520	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_I8520	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I8520	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_I8520	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I8520	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I8520	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I8520	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I8520	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I8520	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_I8520	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I8520	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8520	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8520	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8520	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8520	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I8520	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8520	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8520	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_I8520	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I8520	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I8520	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I8520	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I8520	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I8520	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_I8520	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I8520	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I8520	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I8520	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I8520	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I8520	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
>BBa_I8520
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgaaaatcgaagaag
gtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagatac
cggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccct
gacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaag
cgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatcgctgt
tgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgctggat
aaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgattgctg
ctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaa
agcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcagaagct
gcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagtgaatt
atggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgc
cgccagtccgaacaaagagctggcaaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcggttaat
aaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatccacgtattgccgccacta
tggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggt
gatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactaattcgagctcgaacaacaac
aacaataacaataacaacaacctcgggatcgagggaaggatgaaaccgttccagtgccgtatctgcatgcgtaacttct
cccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaa
attcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactg
ctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgttaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagtttattcttgacatggtccca
cgcgcgtgggatactacgtcagtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactg
gagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtga
tgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcact
actttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgc
ccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttga
aggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattg
gaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatta
gacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcct
tttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt
cttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttata