LOCUS BBa_I8525 2433 bp DNA linear 23-Dec-2024 DEFINITION 3OC6HSL->Inverter->lacZalpha ACCESSION BBa_I8525 VERSION BBa_I8525.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 RBS 1070..1084 /label=RBS-1\Strong RBS 1077..1081 /label=RBS misc_feature 1070..1084 /label=BBa_B0030 RBS 1070..1084 /label=BBa_B0030 CDS 1091..1357 /label=Zif268.GCN4 misc_feature 1094..1180 /label=finger 2 of Zif268 misc_feature 1181..1240 /label=finger 3 of Zif268 misc_feature 1091..1093 /label=start codon misc_feature 1241..1255 /label=domain linker misc_feature 1256..1351 /label=GCN4 leucine zipper homodimerization domain misc_feature 1352..1357 /label=double stop codon CDS 1091..1357 /label=BBa_C2005 stem_loop 1377..1420 /label=stem_loop misc_feature 1366..1445 /label=BBa_B0010 terminator 1366..1445 /label=BBa_B0010 stem_loop 1461..1480 /label=T7 TE misc_feature 1487..1487 /label=stop polyA_site 1481..1494 /label=polya misc_feature 1454..1494 /label=BBa_B0012 terminator 1454..1494 /label=BBa_B0012 promoter 1512..1517 /label=-35 promoter 1535..1540 /label=-10 misc_feature 1522..1535 /label=Zif23 misc_feature 1503..1511 /label=consensus misc_feature 1547..1547 /label=(+1) promoter 1503..1547 /label=BBa_R2000 misc_feature 1070..1547 /label=BBa_Q20050 misc_feature 1..1547 /label=BBa_P20050 RBS 1556..1570 /label=RBS-1\Strong RBS 1563..1567 /label=RBS misc_feature 1556..1570 /label=BBa_B0030 RBS 1556..1570 /label=BBa_B0030 CDS 1577..2296 /label=GFP protein CDS 1577..2296 /label=BBa_E0040 stem_loop 2316..2359 /label=stem_loop misc_feature 2305..2384 /label=BBa_B0010 terminator 2305..2384 /label=BBa_B0010 stem_loop 2400..2419 /label=T7 TE misc_feature 2426..2426 /label=stop polyA_site 2420..2433 /label=polya misc_feature 2393..2433 /label=BBa_B0012 terminator 2393..2433 /label=BBa_B0012 misc_feature 1556..2433 /label=BBa_E0840 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga 1081 gaaatactag atgaaaccgt tccagtgccg tatctgcatg cgtaacttct cccgttccga 1141 ccacctgacc acccacatcc gtacccacac cggtgaaaaa ccgttcgctt gcgacatctg 1201 cggtcgtaaa ttcgctcgtt ccgacgaacg taaacgtcac cgtaaattgc agcacatgaa 1261 acagctggaa gacaaagttg aagaactgct gtccaaaaac taccacctgg aaaacgaagt 1321 tgctcgtctg aaaaaactgg ttggtgaacg ttaataatac tagagccagg catcaaataa 1381 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1441 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 1501 agagtttatt cttgacatgg tcccacgcgc gtgggatact acgtcagtac tagagattaa 1561 agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct 1621 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg 1681 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt 1741 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc 1801 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga 1861 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga 1921 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa 1981 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga 2041 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag 2101 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt 2161 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag 2221 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga 2281 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 2341 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 2401 gctcaccttc gggtgggcct ttctgcgttt ata //