##gff-version 3
##sequence-region BBa_I8540 1 2525
BBa_I8540	.	CDS	1669	2388	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I8540	.	CDS	1669	2388	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I8540	.	terminator	1458	1537	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8540	.	engineered_region	1458	1537	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8540	.	stem_loop	1469	1512	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8540	.	promoter	1595	1639	.	+	0	ID=BBa_R2000;Name=BBa_R2000
BBa_I8540	.	promoter	1627	1632	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_I8540	.	promoter	1604	1609	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_I8540	.	start_codon	1639	1639	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_I8540	.	non_covalent_binding_site	1614	1627	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_I8540	.	sequence_feature	1595	1603	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_I8540	.	ribosome_entry_site	1648	1662	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I8540	.	ribosome_entry_site	1655	1659	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I8540	.	engineered_region	1648	1662	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I8540	.	ribosome_entry_site	1648	1662	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I8540	.	terminator	2485	2525	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8540	.	stop_codon	2518	2518	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8540	.	polyA_site	2512	2525	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8540	.	stem_loop	2492	2511	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8540	.	engineered_region	2485	2525	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I8540	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I8540	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I8540	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I8540	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I8540	.	CDS	22	1449	.	+	0	ID=BBa_C2006;Name=BBa_C2006
BBa_I8540	.	terminator	2397	2476	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I8540	.	engineered_region	2397	2476	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I8540	.	stem_loop	2408	2451	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I8540	.	terminator	1546	1586	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I8540	.	stop_codon	1579	1579	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I8540	.	polyA_site	1573	1586	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I8540	.	stem_loop	1553	1572	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I8540	.	engineered_region	1546	1586	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_I8540
attaaagaggagaaatactagatgaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacg
gtctcgctgaagtcggtaagaaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaaga
gaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgct
caatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtac
gttacaacggcaagctgattgcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaa
cccgccaaaaacctgggaagagatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaac
ctgcaagaaccgtacttcacctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacg
acattaaagacgtgggcgtggataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaaca
catgaatgcagacaccgattactccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccg
tgggcatggtccaacatcgacaccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatcca
aaccgttcgttggcgtgctgagcgcaggtattaacgccgccagtccgaacaaagagctggcaaaagagttcctcgaaaa
ctatctgctgactgatgaaggtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgag
gaagagttggcgaaagatccacgtattgccgccactatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgc
agatgtccgctttctggtatgccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccct
gaaagacgcgcagactaattcgagctcgaacaacaacaacaataacaataacaacaacctcgggatcgagggaaggatg
aaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccg
gtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgca
gcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctg
aaaaaactggttggtgaacgttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagagtttattcttgacatggtcccacgcgcgtgggatactacgtcagtactagagattaaagaggag
aaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatg
ggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactac
tggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagat
catatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatg
acgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattga
ttttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagac
aaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatt
atcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttc
gaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggat
gaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata