LOCUS BBa_I9101 4829 bp DNA linear 23-Dec-2024 DEFINITION test (mnt QPI) I0500.E0422.I0500.I9001.E0432 ACCESSION BBa_I9101 VERSION BBa_I9101.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1998 /label=CFP (LVA) misc_feature 1955..1992 /label=SsrA misc_feature 1993..1998 /label=2 misc_feature 1305..1305 /label=A misc_feature 1237..1998 /label=BBa_E0022 misc_feature 1237..1998 /label=BBa_E0022 stem_loop 2018..2061 /label=stem_loop misc_feature 2007..2086 /label=BBa_B0010 terminator 2007..2086 /label=BBa_B0010 stem_loop 2102..2121 /label=T7 TE misc_feature 2128..2128 /label=stop polyA_site 2122..2135 /label=polya misc_feature 2095..2135 /label=BBa_B0012 terminator 2095..2135 /label=BBa_B0012 promoter 3223..3353 /label=Pbad promoter 3186..3257 /label=PC misc_feature 2144..3222 /label=AraC promoter 2144..3353 /label=BBa_I0500 misc_feature 3366..3369 /label=conserved RBS 3362..3373 /label=BBa_B0034 CDS 3380..3628 /label=mnt misc_feature 3629..3661 /label=LVA misc_feature 3410..3412 /label=Arg->Lys (ctg) misc_feature 3474..3476 /label=T misc_feature 3668..3692 /label=Help:Barcodes CDS 3380..3667 /label=BBa_C0073 stem_loop 3712..3755 /label=stem_loop misc_feature 3701..3780 /label=BBa_B0010 terminator 3701..3780 /label=BBa_B0010 stem_loop 3796..3815 /label=T7 TE misc_feature 3822..3822 /label=stop polyA_site 3816..3829 /label=polya misc_feature 3789..3829 /label=BBa_B0012 terminator 3789..3829 /label=BBa_B0012 misc_feature 3838..3904 /label=mnt misc_feature 3860..3866 /label=dimer left half misc_feature 3869..3875 /label=dimer right half promoter 3884..3889 /label=-10 promoter 3838..3904 /label=BBa_R0073 misc_feature 3917..3920 /label=conserved RBS 3913..3924 /label=BBa_B0034 CDS 3931..4692 /label=YFP (LVA) misc_feature 4687..4692 /label=2 misc_feature 4648..4686 /label=SsrA misc_feature 4172..4172 /label=A (Q->L) misc_feature 3931..4692 /label=BBa_E0032 misc_feature 3931..4692 /label=BBa_E0032 stem_loop 4712..4755 /label=stem_loop misc_feature 4701..4780 /label=BBa_B0010 terminator 4701..4780 /label=BBa_B0010 stem_loop 4796..4815 /label=T7 TE misc_feature 4822..4822 /label=stop polyA_site 4816..4829 /label=polya misc_feature 4789..4829 /label=BBa_B0012 terminator 4789..4829 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 1981 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2101 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttatgac aacttgacgg 2161 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc 2221 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg 2281 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct 2341 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg 2401 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat 2461 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt 2521 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg 2581 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg 2641 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa 2701 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag 2761 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg 2821 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat 2881 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg 2941 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg 3001 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt 3061 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca 3121 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa 3181 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg 3241 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 3301 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agctactaga 3361 gaaagaggag aaatactaga tggcccggga tgatcctcac ttcaatttta aaatgccaat 3421 ggaagtaaga gagaaattga aatttagagc agaggcaaac ggacggagca tgaactctga 3481 gcttttgcaa atcgtacaag atgccctaag caaaccgtca ccagtcactg ggtaccgcaa 3541 tgatgcggaa cgactcgccg atgagcagag cgagttagtg aagaagatgg tcttcgatac 3601 actgaaggat ctttataaaa aaaccaccgc tgcaaacgac gaaaactacg ctttagtagc 3661 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 3721 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 3781 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagctc 3841 gaggtgagtg cacagtacta ggtccacggt gacctagatc tcctatagtg agtcgtatta 3901 attttactag agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc 3961 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 4021 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 4081 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa 4141 tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc 4201 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 4261 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 4321 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 4381 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 4441 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 4501 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa 4561 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 4621 actctcggca tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta 4681 gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 4741 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 4801 accttcgggt gggcctttct gcgtttata //