LOCUS BBa_I9102 4974 bp DNA linear 22-Nov-2024 DEFINITION test (penI QPI) I0500.E0422.I0500.I9002.E0432 ACCESSION BBa_I9102 VERSION BBa_I9102.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1998 /label=CFP (LVA) misc_feature 1955..1992 /label=SsrA misc_feature 1993..1998 /label=2 misc_feature 1305..1305 /label=A misc_feature 1237..1998 /label=BBa_E0022 misc_feature 1237..1998 /label=BBa_E0022 stem_loop 2018..2061 /label=stem_loop misc_feature 2007..2086 /label=BBa_B0010 terminator 2007..2086 /label=BBa_B0010 stem_loop 2102..2121 /label=T7 TE misc_feature 2128..2128 /label=stop polyA_site 2122..2135 /label=polya misc_feature 2095..2135 /label=BBa_B0012 terminator 2095..2135 /label=BBa_B0012 promoter 3223..3353 /label=Pbad promoter 3186..3257 /label=PC misc_feature 2144..3222 /label=AraC promoter 2144..3353 /label=BBa_I0500 misc_feature 3366..3369 /label=conserved RBS 3362..3373 /label=BBa_B0034 CDS 3380..3766 /label=penI misc_feature 3764..3796 /label=LVA misc_feature 3803..3827 /label=Help:Barcodes CDS 3380..3802 /label=BBa_C0074 stem_loop 3847..3890 /label=stem_loop misc_feature 3836..3915 /label=BBa_B0010 terminator 3836..3915 /label=BBa_B0010 stem_loop 3931..3950 /label=T7 TE misc_feature 3957..3957 /label=stop polyA_site 3951..3964 /label=polya misc_feature 3924..3964 /label=BBa_B0012 terminator 3924..3964 /label=BBa_B0012 misc_feature 3973..4049 /label=PenI promoter 3995..4000 /label=-35 promoter 4018..4023 /label=-10 misc_feature 4003..4025 /label=dimer left half misc_feature 4027..4048 /label=dimer right half promoter 3973..4049 /label=BBa_R0074 misc_feature 4062..4065 /label=conserved RBS 4058..4069 /label=BBa_B0034 CDS 4076..4837 /label=YFP (LVA) misc_feature 4832..4837 /label=2 misc_feature 4793..4831 /label=SsrA misc_feature 4317..4317 /label=A (Q->L) misc_feature 4076..4837 /label=BBa_E0032 misc_feature 4076..4837 /label=BBa_E0032 stem_loop 4857..4900 /label=stem_loop misc_feature 4846..4925 /label=BBa_B0010 terminator 4846..4925 /label=BBa_B0010 stem_loop 4941..4960 /label=T7 TE misc_feature 4967..4967 /label=stop polyA_site 4961..4974 /label=polya misc_feature 4934..4974 /label=BBa_B0012 terminator 4934..4974 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 1981 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2101 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttatgac aacttgacgg 2161 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc 2221 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg 2281 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct 2341 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg 2401 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat 2461 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt 2521 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg 2581 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg 2641 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa 2701 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag 2761 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg 2821 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat 2881 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg 2941 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg 3001 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt 3061 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca 3121 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa 3181 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg 3241 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 3301 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agctactaga 3361 gaaagaggag aaatactaga tgaaaaaaat accacagatt tctgatgcgg aactcgaagt 3421 catgaaagtg atttggaagc attcttccat taatactaat gaggtaatca aagagctcag 3481 taaaacttca acgtggagcc caaaaactat tcagactatg ctgctgcgcc ttatcaaaaa 3541 aggcgctctc aaccaccata aagaaggtcg ggttttcgtt tacacgccta atatagacga 3601 atcagattat atagaggtta agtcacactc atttctcaac cggttttaca atggtacatt 3661 aaattccatg gtactcaact ttttggagaa tgatcaactg tcgggagaag aaatcaatga 3721 attgtatcag atactcgaag aacataagaa ccgtaagaag gaagctgcaa acgacgaaaa 3781 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagccagg 3841 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 3901 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 3961 tatatactag agtcatttcc aaccgaaaaa aggcttgcat ttaaatctta catatgtaat 4021 actttcaaag actacatttg taagatttgt actagagaaa gaggagaaat actagatggt 4081 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 4141 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 4201 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 4261 gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca 4321 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 4381 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 4441 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 4501 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 4561 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 4621 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 4681 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 4741 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agaggcctgc 4801 tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg catcaaataa 4861 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 4921 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //