##gff-version 3
##sequence-region BBa_I9102 1 4974
BBa_I9102	.	terminator	2007	2086	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9102	.	stem_loop	2018	2061	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9102	.	engineered_region	2007	2086	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9102	.	promoter	3973	4049	.	+	0	ID=BBa_R0074;Name=BBa_R0074
BBa_I9102	.	promoter	4018	4023	.	+	0	ID=annotation319212;Name=-10;Parent=BBa_R0074
BBa_I9102	.	non_covalent_binding_site	4027	4048	.	+	0	ID=annotation319789;Name=dimer right half;Parent=BBa_R0074
BBa_I9102	.	operator	3973	4049	.	+	0	ID=annotation302713;Name=PenI;Parent=BBa_R0074
BBa_I9102	.	non_covalent_binding_site	4003	4025	.	+	0	ID=annotation319788;Name=dimer left half;Parent=BBa_R0074
BBa_I9102	.	promoter	3995	4000	.	+	0	ID=annotation319211;Name=-35;Parent=BBa_R0074
BBa_I9102	.	terminator	4846	4925	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9102	.	stem_loop	4857	4900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9102	.	engineered_region	4846	4925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9102	.	ribosome_entry_site	3362	3373	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9102	.	conserved_region	3366	3369	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9102	.	ribosome_entry_site	4058	4069	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9102	.	conserved_region	4062	4065	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9102	.	terminator	4934	4974	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9102	.	stem_loop	4941	4960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9102	.	stop_codon	4967	4967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9102	.	polyA_site	4961	4974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9102	.	engineered_region	4934	4974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9102	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I9102	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I9102	.	promoter	2144	3353	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I9102	.	promoter	3186	3257	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I9102	.	promoter	3223	3353	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I9102	.	sequence_feature	2144	3222	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I9102	.	terminator	2095	2135	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9102	.	stem_loop	2102	2121	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9102	.	stop_codon	2128	2128	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9102	.	polyA_site	2122	2135	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9102	.	engineered_region	2095	2135	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9102	.	engineered_region	1237	1998	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I9102	.	tag	1955	1992	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I9102	.	engineered_region	1237	1998	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I9102	.	stop_codon	1993	1998	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I9102	.	point_mutation	1305	1305	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I9102	.	CDS	1237	1998	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I9102	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I9102	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I9102	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I9102	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I9102	.	terminator	3836	3915	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I9102	.	stem_loop	3847	3890	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I9102	.	engineered_region	3836	3915	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I9102	.	terminator	3924	3964	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I9102	.	stem_loop	3931	3950	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I9102	.	stop_codon	3957	3957	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I9102	.	polyA_site	3951	3964	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I9102	.	engineered_region	3924	3964	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I9102	.	engineered_region	4076	4837	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I9102	.	sequence_alteration	4317	4317	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I9102	.	engineered_region	4076	4837	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I9102	.	tag	4793	4831	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I9102	.	stop_codon	4832	4837	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I9102	.	CDS	4076	4837	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I9102	.	CDS	3380	3802	.	+	0	ID=BBa_C0074;Name=BBa_C0074
BBa_I9102	.	tag	3764	3796	.	+	0	ID=annotation306563;Name=LVA;Parent=BBa_C0074
BBa_I9102	.	engineered_tag	3803	3827	.	+	0	ID=annotation2214009;Name=Help:Barcodes;Parent=BBa_C0074
BBa_I9102	.	CDS	3380	3766	.	+	0	ID=annotation302638;Name=penI;Parent=BBa_C0074
>BBa_I9102
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttca
ccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaggg
cgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg
accaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcca
tgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagtt
cgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaag
ctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaaga
tccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgt
gctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtc
ctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaa
actacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggc
cccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggc
atccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaact
gctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtc
tgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgct
tccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgt
taatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatat
tgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctcc
ggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccct
gatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaa
atcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggga
tcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacatt
gccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaag
cgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcac
ggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactct
ctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaaaataccacag
atttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagc
tcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaacca
ccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcattt
ctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaa
tcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagt
agcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaa
gactacatttgtaagatttgtactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggg
gtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatg
ccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccac
cttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgccc
gaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagg
gcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctgga
gtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgc
cacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgc
tgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgct
ggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactac
gctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata