BBa_I9102
type: ComponentDefinition
persistent identity: BBa I9102
display id: BBa_I9102
version: 1
was generated by: iGEM to SBOL conversion
description: test (penI QPI) I0500.E0422.I0500.I9002.E0432
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: crackdots
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_C0074
component: BBa_B0034
component: BBa_I0500
component: BBa_B0012
component: BBa_B0010
component: BBa_E0022
component: BBa_E0032
component: BBa_R0074
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0074
sequence annotation: BBa_R0074
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I9102_sequence/1

BBa_C0074
type: Component
persistent identity: component981154
display id: component981154
version: 1
top level: BBa_I9102
definition: penI
access: public

BBa_B0034
type: Component
persistent identity: component981144
display id: component981144
version: 1
top level: BBa_I9102
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component981139
display id: component981139
version: 1
top level: BBa_I9102
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component981134
display id: component981134
version: 1
top level: BBa_I9102
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component981124
display id: component981124
version: 1
top level: BBa_I9102
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component981116
display id: component981116
version: 1
top level: BBa_I9102
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component981104
display id: component981104
version: 1
top level: BBa_I9102
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component981099
display id: component981099
version: 1
top level: BBa_I9102
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component981225
display id: component981225
version: 1
top level: BBa_I9102
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component981215
display id: component981215
version: 1
top level: BBa_I9102
definition: BBa_B0010
access: public

BBa_E0032
type: Component
persistent identity: component981207
display id: component981207
version: 1
top level: BBa_I9102
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component981195
display id: component981195
version: 1
top level: BBa_I9102
definition: BBa_B0034
access: public

BBa_R0074
type: Component
persistent identity: component981190
display id: component981190
version: 1
top level: BBa_I9102
definition: PenI
access: public

BBa_B0012
type: Component
persistent identity: component981170
display id: component981170
version: 1
top level: BBa_I9102
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component981160
display id: component981160
version: 1
top level: BBa_I9102
definition: BBa_B0010
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation981099
display id: annotation981099
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981099/range981099/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9102/annotation981099/range981099/1
type: Range
persistent identity: range981099
display id: range981099
version: 1
top level: BBa_I9102
direction: direction/forward
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation981104
display id: annotation981104
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981104/range981104/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9102/annotation981104/range981104/1
type: Range
persistent identity: range981104
display id: range981104
version: 1
top level: BBa_I9102
direction: direction/forward
start: 1219
end: 1230
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation981116
display id: annotation981116
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981116/range981116/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I9102/annotation981116/range981116/1
type: Range
persistent identity: range981116
display id: range981116
version: 1
top level: BBa_I9102
direction: direction/forward
start: 1237
end: 1998
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation981124
display id: annotation981124
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981124/range981124/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9102/annotation981124/range981124/1
type: Range
persistent identity: range981124
display id: range981124
version: 1
top level: BBa_I9102
direction: direction/forward
start: 2007
end: 2086
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation981134
display id: annotation981134
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981134/range981134/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9102/annotation981134/range981134/1
type: Range
persistent identity: range981134
display id: range981134
version: 1
top level: BBa_I9102
direction: direction/forward
start: 2095
end: 2135
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation981139
display id: annotation981139
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981139/range981139/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9102/annotation981139/range981139/1
type: Range
persistent identity: range981139
display id: range981139
version: 1
top level: BBa_I9102
direction: direction/forward
start: 2144
end: 3353
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation981144
display id: annotation981144
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981144/range981144/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9102/annotation981144/range981144/1
type: Range
persistent identity: range981144
display id: range981144
version: 1
top level: BBa_I9102
direction: direction/forward
start: 3362
end: 3373
orientation: inline

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation981154
display id: annotation981154
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981154/range981154/1
component: BBa_C0074

https://synbiohub.org/public/igem/BBa_I9102/annotation981154/range981154/1
type: Range
persistent identity: range981154
display id: range981154
version: 1
top level: BBa_I9102
direction: direction/forward
start: 3380
end: 3802
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation981160
display id: annotation981160
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981160/range981160/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9102/annotation981160/range981160/1
type: Range
persistent identity: range981160
display id: range981160
version: 1
top level: BBa_I9102
direction: direction/forward
start: 3836
end: 3915
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation981170
display id: annotation981170
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981170/range981170/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9102/annotation981170/range981170/1
type: Range
persistent identity: range981170
display id: range981170
version: 1
top level: BBa_I9102
direction: direction/forward
start: 3924
end: 3964
orientation: inline

BBa_R0074
type: SequenceAnnotation
persistent identity: annotation981190
display id: annotation981190
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981190/range981190/1
component: BBa_R0074

https://synbiohub.org/public/igem/BBa_I9102/annotation981190/range981190/1
type: Range
persistent identity: range981190
display id: range981190
version: 1
top level: BBa_I9102
direction: direction/forward
start: 3973
end: 4049
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation981195
display id: annotation981195
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981195/range981195/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9102/annotation981195/range981195/1
type: Range
persistent identity: range981195
display id: range981195
version: 1
top level: BBa_I9102
direction: direction/forward
start: 4058
end: 4069
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation981207
display id: annotation981207
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981207/range981207/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I9102/annotation981207/range981207/1
type: Range
persistent identity: range981207
display id: range981207
version: 1
top level: BBa_I9102
direction: direction/forward
start: 4076
end: 4837
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation981215
display id: annotation981215
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981215/range981215/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9102/annotation981215/range981215/1
type: Range
persistent identity: range981215
display id: range981215
version: 1
top level: BBa_I9102
direction: direction/forward
start: 4846
end: 4925
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation981225
display id: annotation981225
version: 1
top level: BBa_I9102
location: https://synbiohub.org/public/igem/BBa_I9102/annotation981225/range981225/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9102/annotation981225/range981225/1
type: Range
persistent identity: range981225
display id: range981225
version: 1
top level: BBa_I9102
direction: direction/forward
start: 4934
end: 4974
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (PenI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: extends slightly past -50
star: true
creator: crackdots
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: PenI
sequence annotation: -35
sequence annotation: dimer left half
sequence annotation: -10
sequence annotation: dimer right half
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
top level: PenI
direction: direction/forward
start: 1
end: 77
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
top level: PenI
direction: direction/forward
start: 23
end: 28
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
top level: PenI
direction: direction/forward
start: 31
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
top level: PenI
direction: direction/forward
start: 46
end: 51
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
top level: PenI
direction: direction/forward
start: 55
end: 76
orientation: inline

penI
type: ComponentDefinition
persistent identity: BBa C0074
display id: BBa_C0074
version: 1
was generated by: iGEM to SBOL conversion
description: penI repressor from Bacillus licheniformis (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Since penI is being ported from Bacillus Licheniformis, the sequence was changed within the coding region to reflect codon usage in E.Coli K12. Codons were mapped to the codon of the same usage rank in E.Coli.
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: penI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0074_sequence/1

penI
type: SequenceAnnotation
persistent identity: annotation302638
display id: annotation302638
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
type: Range
persistent identity: range302638
display id: range302638
version: 1
top level: penI
direction: direction/forward
start: 1
end: 387
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306563
display id: annotation306563
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
type: Range
persistent identity: range306563
display id: range306563
version: 1
top level: penI
direction: direction/forward
start: 385
end: 417
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214009
display id: annotation2214009
version: 1
top level: penI
location: https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
type: Range
persistent identity: range2214009
display id: range2214009
version: 1
top level: penI
direction: direction/forward
start: 424
end: 448
orientation: inline

https://synbiohub.org/public/igem/BBa_I9102_sequence/1
type: Sequence
persistent identity: BBa I9102 sequence
display id: BBa_I9102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0074_sequence/1
type: Sequence
persistent identity: BBa R0074 sequence
display id: BBa_R0074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatttccaaccgaaaaaaggcttgcatttaaatcttacatatgtaatactttcaaagactacatttgtaagatttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0074_sequence/1
type: Sequence
persistent identity: BBa C0074 sequence
display id: BBa_C0074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaaaataccacagatttctgatgcggaactcgaagtcatgaaagtgatttggaagcattcttccattaatactaatgaggtaatcaaagagctcagtaaaacttcaacgtggagcccaaaaactattcagactatgctgctgcgccttatcaaaaaaggcgctctcaaccaccataaagaaggtcgggttttcgtttacacgcctaatatagacgaatcagattatatagaggttaagtcacactcatttctcaaccggttttacaatggtacattaaattccatggtactcaactttttggagaatgatcaactgtcgggagaagaaatcaatgaattgtatcagatactcgaagaacataagaaccgtaagaaggaagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z