LOCUS BBa_I9103 5170 bp DNA linear 22-Nov-2024 DEFINITION test (tp901 cI QPI) I0500.E0422.I0500.I9003.E0432 ACCESSION BBa_I9103 VERSION BBa_I9103.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1998 /label=CFP (LVA) misc_feature 1955..1992 /label=SsrA misc_feature 1993..1998 /label=2 misc_feature 1305..1305 /label=A misc_feature 1237..1998 /label=BBa_E0022 misc_feature 1237..1998 /label=BBa_E0022 stem_loop 2018..2061 /label=stem_loop misc_feature 2007..2086 /label=BBa_B0010 terminator 2007..2086 /label=BBa_B0010 stem_loop 2102..2121 /label=T7 TE misc_feature 2128..2128 /label=stop polyA_site 2122..2135 /label=polya misc_feature 2095..2135 /label=BBa_B0012 terminator 2095..2135 /label=BBa_B0012 promoter 3223..3353 /label=Pbad promoter 3186..3257 /label=PC misc_feature 2144..3222 /label=AraC promoter 2144..3353 /label=BBa_I0500 misc_feature 3366..3369 /label=conserved RBS 3362..3373 /label=BBa_B0034 CDS 3380..3919 /label=cI tp901 misc_feature 3920..3952 /label=LVA misc_feature 3959..3983 /label=Help:Barcodes CDS 3380..3958 /label=BBa_C0075 stem_loop 4003..4046 /label=stem_loop misc_feature 3992..4071 /label=BBa_B0010 terminator 3992..4071 /label=BBa_B0010 stem_loop 4087..4106 /label=T7 TE misc_feature 4113..4113 /label=stop polyA_site 4107..4120 /label=polya misc_feature 4080..4120 /label=BBa_B0012 terminator 4080..4120 /label=BBa_B0012 misc_feature 4147..4185 /label=cI tp901 OR promoter 4181..4187 /label=-35 misc_feature 4213..4243 /label=cI tp901 OL promoter 4204..4210 /label=-10 misc_feature 4217..4217 /label=start promoter 4129..4245 /label=BBa_R0075 misc_feature 4258..4261 /label=conserved RBS 4254..4265 /label=BBa_B0034 CDS 4272..5033 /label=YFP (LVA) misc_feature 5028..5033 /label=2 misc_feature 4989..5027 /label=SsrA misc_feature 4513..4513 /label=A (Q->L) misc_feature 4272..5033 /label=BBa_E0032 misc_feature 4272..5033 /label=BBa_E0032 stem_loop 5053..5096 /label=stem_loop misc_feature 5042..5121 /label=BBa_B0010 terminator 5042..5121 /label=BBa_B0010 stem_loop 5137..5156 /label=T7 TE misc_feature 5163..5163 /label=stop polyA_site 5157..5170 /label=polya misc_feature 5130..5170 /label=BBa_B0012 terminator 5130..5170 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 1981 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2101 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttatgac aacttgacgg 2161 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc 2221 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg 2281 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct 2341 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg 2401 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat 2461 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt 2521 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg 2581 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg 2641 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa 2701 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag 2761 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg 2821 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat 2881 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg 2941 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg 3001 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt 3061 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca 3121 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa 3181 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg 3241 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 3301 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agctactaga 3361 gaaagaggag aaatactaga tgaaaaccga taccagtaac agattaaaac aaataatggc 3421 ggaacgcaac ctgaaacagg tggatatctt aaacttatcg attccatttc agaaaaaatt 3481 tggtatcaag ctgagtaaaa gtacgctgtc gcagtatgtg aactctgtgc agtcgccaga 3541 ccagaaccgc atctatttac tggcgaaaac gctgggcgtt tccgaagctt ggctgatggg 3601 atttgatgtt ccaatggttg aatcgtcgaa aatcgaaaac gattcggaaa acatcgaaga 3661 aaccattacg gtgatgaaaa aattggaaga gccgagacag aaagtggtcc tggataccgc 3721 caaaattcag ctgaaagaac aggatgagca gaacaaagtg aaacagatcg aagattaccg 3781 tctaagtgat gaatatttag aagaacagat cagtaaagcg agtgcatatg gaggtggcca 3841 attaaatgac aacgataaag aatttttcaa acgtttgttg aaaaacacgt taaaagaaaa 3901 aattgataaa ggagatctgg ctgcaaacga cgaaaactac gctttagtag cttaataaca 3961 ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag 4021 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 4081 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagcg catcttgaac 4141 aaaagttcaa caaaaaaatt catatttcgt gaactttttt gttgacaaag ataaaaacac 4201 atgatatact aatttcataa agttcatgaa acgtgaactg aaatttacta gagaaagagg 4261 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 4321 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 4381 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 4441 cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg 4501 accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 4561 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 4621 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 4681 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 4741 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 4801 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 4861 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg 4921 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 4981 tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga 5041 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5101 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 5161 tgcgtttata //