LOCUS BBa_I9108 9298 bp DNA linear 22-Dec-2024 DEFINITION test (tp901-cI nor) I9107.I9106.I9106 ACCESSION BBa_I9108 VERSION BBa_I9108.1 FEATURES Location/Qualifiers misc_feature 19..57 /label=cI tp901 OR promoter 53..59 /label=-35 misc_feature 85..115 /label=cI tp901 OL promoter 76..82 /label=-10 misc_feature 89..89 /label=start promoter 1..117 /label=BBa_R0075 misc_feature 130..133 /label=conserved RBS 126..137 /label=BBa_B0034 CDS 144..905 /label=YFP (LVA) misc_feature 900..905 /label=2 misc_feature 861..899 /label=SsrA misc_feature 385..385 /label=A (Q->L) misc_feature 144..905 /label=BBa_E0032 misc_feature 144..905 /label=BBa_E0032 stem_loop 925..968 /label=stem_loop misc_feature 914..993 /label=BBa_B0010 terminator 914..993 /label=BBa_B0010 stem_loop 1009..1028 /label=T7 TE misc_feature 1035..1035 /label=stop polyA_site 1029..1042 /label=polya misc_feature 1002..1042 /label=BBa_B0012 terminator 1002..1042 /label=BBa_B0012 promoter 2130..2260 /label=Pbad promoter 2093..2164 /label=PC misc_feature 1051..2129 /label=AraC promoter 1051..2260 /label=BBa_I0500 misc_feature 2273..2276 /label=conserved RBS 2269..2280 /label=BBa_B0034 CDS 2287..3048 /label=CFP (LVA) misc_feature 3005..3042 /label=SsrA misc_feature 3043..3048 /label=2 misc_feature 2355..2355 /label=A misc_feature 2287..3048 /label=BBa_E0022 misc_feature 2287..3048 /label=BBa_E0022 stem_loop 3068..3111 /label=stem_loop misc_feature 3057..3136 /label=BBa_B0010 terminator 3057..3136 /label=BBa_B0010 stem_loop 3152..3171 /label=T7 TE misc_feature 3178..3178 /label=stop polyA_site 3172..3185 /label=polya misc_feature 3145..3185 /label=BBa_B0012 terminator 3145..3185 /label=BBa_B0012 promoter 4273..4403 /label=Pbad promoter 4236..4307 /label=PC misc_feature 3194..4272 /label=AraC promoter 3194..4403 /label=BBa_I0500 misc_feature 4416..4419 /label=conserved RBS 4412..4423 /label=BBa_B0034 CDS 4430..4969 /label=cI tp901 misc_feature 4970..5002 /label=LVA misc_feature 5009..5033 /label=Help:Barcodes CDS 4430..5008 /label=BBa_C0075 stem_loop 5053..5096 /label=stem_loop misc_feature 5042..5121 /label=BBa_B0010 terminator 5042..5121 /label=BBa_B0010 stem_loop 5137..5156 /label=T7 TE misc_feature 5163..5163 /label=stop polyA_site 5157..5170 /label=polya misc_feature 5130..5170 /label=BBa_B0012 terminator 5130..5170 /label=BBa_B0012 promoter 6258..6388 /label=Pbad promoter 6221..6292 /label=PC misc_feature 5179..6257 /label=AraC promoter 5179..6388 /label=BBa_I0500 misc_feature 6401..6404 /label=conserved RBS 6397..6408 /label=BBa_B0034 CDS 6415..7176 /label=CFP (LVA) misc_feature 7133..7170 /label=SsrA misc_feature 7171..7176 /label=2 misc_feature 6483..6483 /label=A misc_feature 6415..7176 /label=BBa_E0022 misc_feature 6415..7176 /label=BBa_E0022 stem_loop 7196..7239 /label=stem_loop misc_feature 7185..7264 /label=BBa_B0010 terminator 7185..7264 /label=BBa_B0010 stem_loop 7280..7299 /label=T7 TE misc_feature 7306..7306 /label=stop polyA_site 7300..7313 /label=polya misc_feature 7273..7313 /label=BBa_B0012 terminator 7273..7313 /label=BBa_B0012 promoter 8401..8531 /label=Pbad promoter 8364..8435 /label=PC misc_feature 7322..8400 /label=AraC promoter 7322..8531 /label=BBa_I0500 misc_feature 8544..8547 /label=conserved RBS 8540..8551 /label=BBa_B0034 CDS 8558..9097 /label=cI tp901 misc_feature 9098..9130 /label=LVA misc_feature 9137..9161 /label=Help:Barcodes CDS 8558..9136 /label=BBa_C0075 stem_loop 9181..9224 /label=stem_loop misc_feature 9170..9249 /label=BBa_B0010 terminator 9170..9249 /label=BBa_B0010 stem_loop 9265..9284 /label=T7 TE misc_feature 9291..9291 /label=stop polyA_site 9285..9298 /label=polya misc_feature 9258..9298 /label=BBa_B0012 terminator 9258..9298 /label=BBa_B0012 ORIGIN 1 cgcatcttga acaaaagttc aacaaaaaaa ttcatatttc gtgaactttt ttgttgacaa 61 agataaaaac acatgatata ctaatttcat aaagttcatg aaacgtgaac tgaaatttac 121 tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg 181 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 241 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 301 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg 361 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct 421 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 481 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 541 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 601 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 661 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 721 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca 781 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 841 gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt 901 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 961 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 1021 ggtgggcctt tctgcgttta tatactagag ttatgacaac ttgacggcta catcattcac 1081 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac 1141 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat 1201 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa 1261 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac 1321 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg 1381 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat 1441 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc 1501 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc 1561 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca 1621 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg 1681 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa 1741 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc 1801 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt 1861 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc 1921 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat 1981 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc 2041 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa 2101 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg 2161 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca 2221 actctctact gtttctccat acccgttttt ttgggctagc tactagagaa agaggagaaa 2281 tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 2341 ctggacggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 2401 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 2461 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac 2521 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 2581 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 2641 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 2701 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag 2761 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 2821 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 2881 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 2941 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 3001 aagaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag 3061 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 3121 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 3181 ttatatacta gagttatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg 3241 cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt 3301 gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa 3361 gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact 3421 gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg 3481 cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc 3541 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt 3601 gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa 3661 tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc 3721 ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa 3781 agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct 3841 ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt 3901 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc 3961 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat 4021 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca 4081 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact 4141 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc 4201 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg 4261 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt 4321 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc 4381 catacccgtt tttttgggct agctactaga gaaagaggag aaatactaga tgaaaaccga 4441 taccagtaac agattaaaac aaataatggc ggaacgcaac ctgaaacagg tggatatctt 4501 aaacttatcg attccatttc agaaaaaatt tggtatcaag ctgagtaaaa gtacgctgtc 4561 gcagtatgtg aactctgtgc agtcgccaga ccagaaccgc atctatttac tggcgaaaac 4621 gctgggcgtt tccgaagctt ggctgatggg atttgatgtt ccaatggttg aatcgtcgaa 4681 aatcgaaaac gattcggaaa acatcgaaga aaccattacg gtgatgaaaa aattggaaga 4741 gccgagacag aaagtggtcc tggataccgc caaaattcag ctgaaagaac aggatgagca 4801 gaacaaagtg aaacagatcg aagattaccg tctaagtgat gaatatttag aagaacagat 4861 cagtaaagcg agtgcatatg gaggtggcca attaaatgac aacgataaag aatttttcaa 4921 acgtttgttg aaaaacacgt taaaagaaaa aattgataaa ggagatctgg ctgcaaacga 4981 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga 5041 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5101 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 5161 tgcgtttata tactagagtt atgacaactt gacggctaca tcattcactt tttcttcaca 5221 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata 5281 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct 5341 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc 5401 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac 5461 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 5521 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 5581 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 5641 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 5701 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 5761 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 5821 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg 5881 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 5941 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac 6001 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact 6061 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg 6121 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag 6181 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa 6241 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc 6301 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt 6361 ttctccatac ccgttttttt gggctagcta ctagagaaag aggagaaata ctagatggtg 6421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 6481 gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 6541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 6601 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 6661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 6721 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 6781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 6841 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc 6901 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 6961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 7021 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 7081 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctgct 7141 gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa 7201 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 7261 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 7321 gttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc 7381 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac 7441 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct 7501 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa 7561 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat 7621 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg 7681 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat 7741 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa 7801 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa 7861 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact 7921 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga 7981 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct 8041 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa 8101 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg 8161 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat 8221 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact 8281 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg 8341 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc 8401 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 8461 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt 8521 tttgggctag ctactagaga aagaggagaa atactagatg aaaaccgata ccagtaacag 8581 attaaaacaa ataatggcgg aacgcaacct gaaacaggtg gatatcttaa acttatcgat 8641 tccatttcag aaaaaatttg gtatcaagct gagtaaaagt acgctgtcgc agtatgtgaa 8701 ctctgtgcag tcgccagacc agaaccgcat ctatttactg gcgaaaacgc tgggcgtttc 8761 cgaagcttgg ctgatgggat ttgatgttcc aatggttgaa tcgtcgaaaa tcgaaaacga 8821 ttcggaaaac atcgaagaaa ccattacggt gatgaaaaaa ttggaagagc cgagacagaa 8881 agtggtcctg gataccgcca aaattcagct gaaagaacag gatgagcaga acaaagtgaa 8941 acagatcgaa gattaccgtc taagtgatga atatttagaa gaacagatca gtaaagcgag 9001 tgcatatgga ggtggccaat taaatgacaa cgataaagaa tttttcaaac gtttgttgaa 9061 aaacacgtta aaagaaaaaa ttgataaagg agatctggct gcaaacgacg aaaactacgc 9121 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 9181 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 9241 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //