LOCUS BBa_I9111 9539 bp DNA linear 28-Nov-2024 DEFINITION test (lambda-cI nor) I9110.I9109.I9109 ACCESSION BBa_I9111 VERSION BBa_I9111.1 FEATURES Location/Qualifiers misc_feature 1..82 /label=cI lambda promoter 1..82 /label=BBa_I9201 misc_feature 95..98 /label=conserved RBS 91..102 /label=BBa_B0034 CDS 109..870 /label=YFP (LVA) misc_feature 865..870 /label=2 misc_feature 826..864 /label=SsrA misc_feature 350..350 /label=A (Q->L) misc_feature 109..870 /label=BBa_E0032 misc_feature 109..870 /label=BBa_E0032 stem_loop 890..933 /label=stem_loop misc_feature 879..958 /label=BBa_B0010 terminator 879..958 /label=BBa_B0010 stem_loop 974..993 /label=T7 TE misc_feature 1000..1000 /label=stop polyA_site 994..1007 /label=polya misc_feature 967..1007 /label=BBa_B0012 terminator 967..1007 /label=BBa_B0012 promoter 2095..2225 /label=Pbad promoter 2058..2129 /label=PC misc_feature 1016..2094 /label=AraC promoter 1016..2225 /label=BBa_I0500 misc_feature 2238..2241 /label=conserved RBS 2234..2245 /label=BBa_B0034 CDS 2252..3013 /label=CFP (LVA) misc_feature 2970..3007 /label=SsrA misc_feature 3008..3013 /label=2 misc_feature 2320..2320 /label=A misc_feature 2252..3013 /label=BBa_E0022 misc_feature 2252..3013 /label=BBa_E0022 stem_loop 3033..3076 /label=stem_loop misc_feature 3022..3101 /label=BBa_B0010 terminator 3022..3101 /label=BBa_B0010 stem_loop 3117..3136 /label=T7 TE misc_feature 3143..3143 /label=stop polyA_site 3137..3150 /label=polya misc_feature 3110..3150 /label=BBa_B0012 terminator 3110..3150 /label=BBa_B0012 promoter 4238..4368 /label=Pbad promoter 4201..4272 /label=PC misc_feature 3159..4237 /label=AraC promoter 3159..4368 /label=BBa_I0500 misc_feature 4381..4384 /label=conserved RBS 4377..4388 /label=BBa_B0034 CDS 4395..5111 /label=cI lambda misc_feature 5112..5136 /label=Help:Barcodes CDS 4395..5111 /label=BBa_I9200 stem_loop 5156..5199 /label=stem_loop misc_feature 5145..5224 /label=BBa_B0010 terminator 5145..5224 /label=BBa_B0010 stem_loop 5240..5259 /label=T7 TE misc_feature 5266..5266 /label=stop polyA_site 5260..5273 /label=polya misc_feature 5233..5273 /label=BBa_B0012 terminator 5233..5273 /label=BBa_B0012 promoter 6361..6491 /label=Pbad promoter 6324..6395 /label=PC misc_feature 5282..6360 /label=AraC promoter 5282..6491 /label=BBa_I0500 misc_feature 6504..6507 /label=conserved RBS 6500..6511 /label=BBa_B0034 CDS 6518..7279 /label=CFP (LVA) misc_feature 7236..7273 /label=SsrA misc_feature 7274..7279 /label=2 misc_feature 6586..6586 /label=A misc_feature 6518..7279 /label=BBa_E0022 misc_feature 6518..7279 /label=BBa_E0022 stem_loop 7299..7342 /label=stem_loop misc_feature 7288..7367 /label=BBa_B0010 terminator 7288..7367 /label=BBa_B0010 stem_loop 7383..7402 /label=T7 TE misc_feature 7409..7409 /label=stop polyA_site 7403..7416 /label=polya misc_feature 7376..7416 /label=BBa_B0012 terminator 7376..7416 /label=BBa_B0012 promoter 8504..8634 /label=Pbad promoter 8467..8538 /label=PC misc_feature 7425..8503 /label=AraC promoter 7425..8634 /label=BBa_I0500 misc_feature 8647..8650 /label=conserved RBS 8643..8654 /label=BBa_B0034 CDS 8661..9377 /label=cI lambda misc_feature 9378..9402 /label=Help:Barcodes CDS 8661..9377 /label=BBa_I9200 stem_loop 9422..9465 /label=stem_loop misc_feature 9411..9490 /label=BBa_B0010 terminator 9411..9490 /label=BBa_B0010 stem_loop 9506..9525 /label=T7 TE misc_feature 9532..9532 /label=stop polyA_site 9526..9539 /label=polya misc_feature 9499..9539 /label=BBa_B0012 terminator 9499..9539 /label=BBa_B0012 ORIGIN 1 tgcaatttag atagtggcgt tccctattta tagattgtgg cagcgacaac tgataaaatg 61 gagaccgcca ctattaccaa cgtactagag aaagaggaga aatactagat ggtgagcaag 121 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 181 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 241 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 301 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc 361 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 421 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 481 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 541 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 601 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 721 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 781 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac 841 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa 901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 961 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttatg 1021 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc 1081 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat 1141 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga 1201 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg 1261 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt 1321 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat 1381 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg 1441 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt 1501 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg 1561 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat 1621 accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca 1681 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc 1741 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga 1801 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc 1861 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag 1921 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 1981 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 2041 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 2101 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 2161 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 2221 ctagctacta gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac 2281 cggggtggtg cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt 2341 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 2401 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca 2461 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 2521 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 2581 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 2641 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa 2701 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca 2761 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 2821 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 2881 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 2941 cactctcggc atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt 3001 agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3061 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3121 caccttcggg tgggcctttc tgcgtttata tactagagtt atgacaactt gacggctaca 3181 tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt 3241 ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg 3301 ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc 3361 cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac 3421 aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg 3481 atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc 3541 gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag 3601 cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg 3661 tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat 3721 tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc 3781 ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg 3841 caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga 3901 atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca 3961 atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca 4021 ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca 4081 tattgcatca gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg 4141 gtaaccccgc ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg 4201 cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc 4261 acactttgct atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt 4321 ttatcgcaac tctctactgt ttctccatac ccgttttttt gggctagcta ctagagaaag 4381 aggagaaata ctagatgagc actaaaaaga aaccacttac tcaagagcag ttagaggacg 4441 cccgtcgctt aaaagccatt tatgaaaaaa agaaaaatga attaggcctt tcccaggaat 4501 cagtcgccga caagatgggg atggggcagt cgggcgttgg tgctcttttt aatggcatca 4561 atgcccttaa tgcttataac gcggccctct tagccaaaat tttgaaagtt agcgttgaag 4621 aatttagccc ttcgatcgcg cgagaaatct acgagatgta tgaagcagtt agtatgcagc 4681 cgtcgttacg aagtgagtat gagtaccctg ttttttcaca tgttcaggcc gggatgttct 4741 cgcctgagtt acgaaccttt accaaaggtg atgcagagcg atgggtaagc actaccaaaa 4801 aagcgagtga ttcagccttc tggttagagg ttgaaggtaa ttccatgacc gccccaactg 4861 gctccaagcc aagctttcct gacggaatgc ttattttggt tgaccctgag caggctgttg 4921 agccaggtga tttctgcata gcgcgattag ggggtgatga gtttaccttc aagaaactga 4981 tcagggatag cggtcaggtg tttcttcaac cactaaaccc acagtaccca atgatcccat 5041 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt 5101 ttggctaata actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa 5161 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 5221 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 5281 gttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc 5341 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac 5401 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct 5461 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa 5521 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat 5581 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg 5641 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat 5701 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa 5761 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa 5821 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact 5881 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga 5941 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct 6001 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa 6061 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg 6121 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat 6181 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact 6241 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg 6301 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc 6361 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 6421 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt 6481 tttgggctag ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct 6541 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 6601 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 6661 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 6721 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 6781 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 6841 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 6901 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 6961 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 7021 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 7081 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 7141 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 7201 cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta 7261 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 7321 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 7381 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttatga caacttgacg 7441 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 7501 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 7561 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 7621 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac 7681 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 7741 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 7801 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc 7861 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 7921 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 7981 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 8041 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 8101 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 8161 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 8221 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 8281 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 8341 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 8401 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 8461 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 8521 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga 8581 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagctactag 8641 agaaagagga gaaatactag atgagcacta aaaagaaacc acttactcaa gagcagttag 8701 aggacgcccg tcgcttaaaa gccatttatg aaaaaaagaa aaatgaatta ggcctttccc 8761 aggaatcagt cgccgacaag atggggatgg ggcagtcggg cgttggtgct ctttttaatg 8821 gcatcaatgc ccttaatgct tataacgcgg ccctcttagc caaaattttg aaagttagcg 8881 ttgaagaatt tagcccttcg atcgcgcgag aaatctacga gatgtatgaa gcagttagta 8941 tgcagccgtc gttacgaagt gagtatgagt accctgtttt ttcacatgtt caggccggga 9001 tgttctcgcc tgagttacga acctttacca aaggtgatgc agagcgatgg gtaagcacta 9061 ccaaaaaagc gagtgattca gccttctggt tagaggttga aggtaattcc atgaccgccc 9121 caactggctc caagccaagc tttcctgacg gaatgcttat tttggttgac cctgagcagg 9181 ctgttgagcc aggtgatttc tgcatagcgc gattaggggg tgatgagttt accttcaaga 9241 aactgatcag ggatagcggt caggtgtttc ttcaaccact aaacccacag tacccaatga 9301 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 9361 agacgtttgg ctaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca 9421 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 9481 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //