BBa_I9111
type: ComponentDefinition
persistent identity: BBa I9111
display id: BBa_I9111
version: 1
was generated by: iGEM to SBOL conversion
description: test (lambda-cI nor) I9110.I9109.I9109
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: crackdots
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_I9200
component: BBa_B0034
component: BBa_I0500
component: BBa_B0012
component: BBa_E0022
component: BBa_E0032
component: BBa_I9201
sequence annotation: BBa_I9201
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_E0022
sequence annotation: BBa_I9200
sequence: https://synbiohub.org/public/igem/BBa_I9111_sequence/1

BBa_B0010
type: Component
persistent identity: component995996
display id: component995996
version: 1
top level: BBa_I9111
definition: BBa_B0010
access: public

BBa_I9200
type: Component
persistent identity: component995990
display id: component995990
version: 1
top level: BBa_I9111
definition: cI
access: public

BBa_B0034
type: Component
persistent identity: component995983
display id: component995983
version: 1
top level: BBa_I9111
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component995978
display id: component995978
version: 1
top level: BBa_I9111
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component995973
display id: component995973
version: 1
top level: BBa_I9111
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component995963
display id: component995963
version: 1
top level: BBa_I9111
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component995955
display id: component995955
version: 1
top level: BBa_I9111
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component995943
display id: component995943
version: 1
top level: BBa_I9111
definition: BBa_B0034
access: public

BBa_E0022
type: Component
persistent identity: component995882
display id: component995882
version: 1
top level: BBa_I9111
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component995870
display id: component995870
version: 1
top level: BBa_I9111
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component995865
display id: component995865
version: 1
top level: BBa_I9111
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component996006
display id: component996006
version: 1
top level: BBa_I9111
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component995860
display id: component995860
version: 1
top level: BBa_I9111
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component995850
display id: component995850
version: 1
top level: BBa_I9111
definition: BBa_B0010
access: public

BBa_E0032
type: Component
persistent identity: component995842
display id: component995842
version: 1
top level: BBa_I9111
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component995830
display id: component995830
version: 1
top level: BBa_I9111
definition: BBa_B0034
access: public

BBa_I9201
type: Component
persistent identity: component995825
display id: component995825
version: 1
top level: BBa_I9111
definition: cI lam
access: public

BBa_I0500
type: Component
persistent identity: component995938
display id: component995938
version: 1
top level: BBa_I9111
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component995933
display id: component995933
version: 1
top level: BBa_I9111
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component995923
display id: component995923
version: 1
top level: BBa_I9111
definition: BBa_B0010
access: public

BBa_I9200
type: Component
persistent identity: component995917
display id: component995917
version: 1
top level: BBa_I9111
definition: cI
access: public

BBa_B0034
type: Component
persistent identity: component995910
display id: component995910
version: 1
top level: BBa_I9111
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component995905
display id: component995905
version: 1
top level: BBa_I9111
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component995900
display id: component995900
version: 1
top level: BBa_I9111
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component995890
display id: component995890
version: 1
top level: BBa_I9111
definition: BBa_B0010
access: public

BBa_I9201
type: SequenceAnnotation
persistent identity: annotation995825
display id: annotation995825
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995825/range995825/1
component: BBa_I9201

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type: Range
persistent identity: range995825
display id: range995825
version: 1
top level: BBa_I9111
direction: direction/forward
start: 1
end: 82
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation995830
display id: annotation995830
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995830/range995830/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9111/annotation995830/range995830/1
type: Range
persistent identity: range995830
display id: range995830
version: 1
top level: BBa_I9111
direction: direction/forward
start: 91
end: 102
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation995842
display id: annotation995842
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995842/range995842/1
component: BBa_E0032

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type: Range
persistent identity: range995842
display id: range995842
version: 1
top level: BBa_I9111
direction: direction/forward
start: 109
end: 870
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation995850
display id: annotation995850
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995850/range995850/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9111/annotation995850/range995850/1
type: Range
persistent identity: range995850
display id: range995850
version: 1
top level: BBa_I9111
direction: direction/forward
start: 879
end: 958
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation995860
display id: annotation995860
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995860/range995860/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9111/annotation995860/range995860/1
type: Range
persistent identity: range995860
display id: range995860
version: 1
top level: BBa_I9111
direction: direction/forward
start: 967
end: 1007
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation995865
display id: annotation995865
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995865/range995865/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9111/annotation995865/range995865/1
type: Range
persistent identity: range995865
display id: range995865
version: 1
top level: BBa_I9111
direction: direction/forward
start: 1016
end: 2225
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation995870
display id: annotation995870
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995870/range995870/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9111/annotation995870/range995870/1
type: Range
persistent identity: range995870
display id: range995870
version: 1
top level: BBa_I9111
direction: direction/forward
start: 2234
end: 2245
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation995882
display id: annotation995882
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995882/range995882/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I9111/annotation995882/range995882/1
type: Range
persistent identity: range995882
display id: range995882
version: 1
top level: BBa_I9111
direction: direction/forward
start: 2252
end: 3013
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation995890
display id: annotation995890
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995890/range995890/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9111/annotation995890/range995890/1
type: Range
persistent identity: range995890
display id: range995890
version: 1
top level: BBa_I9111
direction: direction/forward
start: 3022
end: 3101
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation995900
display id: annotation995900
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995900/range995900/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9111/annotation995900/range995900/1
type: Range
persistent identity: range995900
display id: range995900
version: 1
top level: BBa_I9111
direction: direction/forward
start: 3110
end: 3150
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation995905
display id: annotation995905
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995905/range995905/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9111/annotation995905/range995905/1
type: Range
persistent identity: range995905
display id: range995905
version: 1
top level: BBa_I9111
direction: direction/forward
start: 3159
end: 4368
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation995910
display id: annotation995910
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995910/range995910/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9111/annotation995910/range995910/1
type: Range
persistent identity: range995910
display id: range995910
version: 1
top level: BBa_I9111
direction: direction/forward
start: 4377
end: 4388
orientation: inline

BBa_I9200
type: SequenceAnnotation
persistent identity: annotation995917
display id: annotation995917
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995917/range995917/1
component: BBa_I9200

https://synbiohub.org/public/igem/BBa_I9111/annotation995917/range995917/1
type: Range
persistent identity: range995917
display id: range995917
version: 1
top level: BBa_I9111
direction: direction/forward
start: 4395
end: 5111
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation995923
display id: annotation995923
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995923/range995923/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9111/annotation995923/range995923/1
type: Range
persistent identity: range995923
display id: range995923
version: 1
top level: BBa_I9111
direction: direction/forward
start: 5145
end: 5224
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation995933
display id: annotation995933
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995933/range995933/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9111/annotation995933/range995933/1
type: Range
persistent identity: range995933
display id: range995933
version: 1
top level: BBa_I9111
direction: direction/forward
start: 5233
end: 5273
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation995938
display id: annotation995938
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995938/range995938/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9111/annotation995938/range995938/1
type: Range
persistent identity: range995938
display id: range995938
version: 1
top level: BBa_I9111
direction: direction/forward
start: 5282
end: 6491
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation995943
display id: annotation995943
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995943/range995943/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9111/annotation995943/range995943/1
type: Range
persistent identity: range995943
display id: range995943
version: 1
top level: BBa_I9111
direction: direction/forward
start: 6500
end: 6511
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation995955
display id: annotation995955
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995955/range995955/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I9111/annotation995955/range995955/1
type: Range
persistent identity: range995955
display id: range995955
version: 1
top level: BBa_I9111
direction: direction/forward
start: 6518
end: 7279
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation995963
display id: annotation995963
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995963/range995963/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9111/annotation995963/range995963/1
type: Range
persistent identity: range995963
display id: range995963
version: 1
top level: BBa_I9111
direction: direction/forward
start: 7288
end: 7367
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation995973
display id: annotation995973
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995973/range995973/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9111/annotation995973/range995973/1
type: Range
persistent identity: range995973
display id: range995973
version: 1
top level: BBa_I9111
direction: direction/forward
start: 7376
end: 7416
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation995978
display id: annotation995978
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995978/range995978/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9111/annotation995978/range995978/1
type: Range
persistent identity: range995978
display id: range995978
version: 1
top level: BBa_I9111
direction: direction/forward
start: 7425
end: 8634
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation995983
display id: annotation995983
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995983/range995983/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9111/annotation995983/range995983/1
type: Range
persistent identity: range995983
display id: range995983
version: 1
top level: BBa_I9111
direction: direction/forward
start: 8643
end: 8654
orientation: inline

BBa_I9200
type: SequenceAnnotation
persistent identity: annotation995990
display id: annotation995990
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995990/range995990/1
component: BBa_I9200

https://synbiohub.org/public/igem/BBa_I9111/annotation995990/range995990/1
type: Range
persistent identity: range995990
display id: range995990
version: 1
top level: BBa_I9111
direction: direction/forward
start: 8661
end: 9377
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation995996
display id: annotation995996
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation995996/range995996/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9111/annotation995996/range995996/1
type: Range
persistent identity: range995996
display id: range995996
version: 1
top level: BBa_I9111
direction: direction/forward
start: 9411
end: 9490
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation996006
display id: annotation996006
version: 1
top level: BBa_I9111
location: https://synbiohub.org/public/igem/BBa_I9111/annotation996006/range996006/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9111/annotation996006/range996006/1
type: Range
persistent identity: range996006
display id: range996006
version: 1
top level: BBa_I9111
direction: direction/forward
start: 9499
end: 9539
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa I9201
display id: BBa_I9201
version: 1
was generated by: iGEM to SBOL conversion
description: lambda cI operator/binding site
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
mutable provenance: lambda bacteriophage
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: operator sites: O1, O2, and O3
star: false
creator: crackdots
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI lambda
sequence: https://synbiohub.org/public/igem/BBa_I9201_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation302665
display id: annotation302665
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_I9201/annotation302665/range302665/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I9201/annotation302665/range302665/1
type: Range
persistent identity: range302665
display id: range302665
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 82
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

cI
type: ComponentDefinition
persistent identity: BBa I9200
display id: BBa_I9200
version: 1
was generated by: iGEM to SBOL conversion
description: cI lambda
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
mutable provenance: lambda bacteriophage
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: cI sequence slightly different from part C0051. NCBI locus: NC_001416
star: false
creator: crackdots
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cI lambda
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I9200_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation302655
display id: annotation302655
version: 1
top level: cI
location: https://synbiohub.org/public/igem/BBa_I9200/annotation302655/range302655/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I9200/annotation302655/range302655/1
type: Range
persistent identity: range302655
display id: range302655
version: 1
top level: cI
direction: direction/forward
start: 1
end: 717
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214008
display id: annotation2214008
version: 1
top level: cI
location: https://synbiohub.org/public/igem/BBa_I9200/annotation2214008/range2214008/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I9200/annotation2214008/range2214008/1
type: Range
persistent identity: range2214008
display id: range2214008
version: 1
top level: cI
direction: direction/forward
start: 718
end: 742
orientation: inline

https://synbiohub.org/public/igem/BBa_I9111_sequence/1
type: Sequence
persistent identity: BBa I9111 sequence
display id: BBa_I9111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcaatttagatagtggcgttccctatttatagattgtggcagcgacaactgataaaatggagaccgccactattaccaacgtactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9201_sequence/1
type: Sequence
persistent identity: BBa I9201 sequence
display id: BBa_I9201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcaatttagatagtggcgttccctatttatagattgtggcagcgacaactgataaaatggagaccgccactattaccaacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I9200_sequence/1
type: Sequence
persistent identity: BBa I9200 sequence
display id: BBa_I9200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcactaaaaagaaaccacttactcaagagcagttagaggacgcccgtcgcttaaaagccatttatgaaaaaaagaaaaatgaattaggcctttcccaggaatcagtcgccgacaagatggggatggggcagtcgggcgttggtgctctttttaatggcatcaatgcccttaatgcttataacgcggccctcttagccaaaattttgaaagttagcgttgaagaatttagcccttcgatcgcgcgagaaatctacgagatgtatgaagcagttagtatgcagccgtcgttacgaagtgagtatgagtaccctgttttttcacatgttcaggccgggatgttctcgcctgagttacgaacctttaccaaaggtgatgcagagcgatgggtaagcactaccaaaaaagcgagtgattcagccttctggttagaggttgaaggtaattccatgaccgccccaactggctccaagccaagctttcctgacggaatgcttattttggttgaccctgagcaggctgttgagccaggtgatttctgcatagcgcgattagggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttcttcaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z