LOCUS BBa_I9112 6165 bp DNA linear 25-Nov-2024 DEFINITION test (wired_nor_switch 1) I0500.E0422.10500.I9004.I9003.E0432 ACCESSION BBa_I9112 VERSION BBa_I9112.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1998 /label=CFP (LVA) misc_feature 1955..1992 /label=SsrA misc_feature 1993..1998 /label=2 misc_feature 1305..1305 /label=A misc_feature 1237..1998 /label=BBa_E0022 misc_feature 1237..1998 /label=BBa_E0022 stem_loop 2018..2061 /label=stem_loop misc_feature 2007..2086 /label=BBa_B0010 terminator 2007..2086 /label=BBa_B0010 stem_loop 2102..2121 /label=T7 TE misc_feature 2128..2128 /label=stop polyA_site 2122..2135 /label=polya misc_feature 2095..2135 /label=BBa_B0012 terminator 2095..2135 /label=BBa_B0012 promoter 3223..3353 /label=Pbad promoter 3186..3257 /label=PC misc_feature 2144..3222 /label=AraC promoter 2144..3353 /label=BBa_I0500 misc_feature 3366..3369 /label=conserved RBS 3362..3373 /label=BBa_B0034 CDS 3380..4096 /label=cI lambda misc_feature 4097..4121 /label=Help:Barcodes CDS 3380..4096 /label=BBa_I9200 stem_loop 4141..4184 /label=stem_loop misc_feature 4130..4209 /label=BBa_B0010 terminator 4130..4209 /label=BBa_B0010 stem_loop 4225..4244 /label=T7 TE misc_feature 4251..4251 /label=stop polyA_site 4245..4258 /label=polya misc_feature 4218..4258 /label=BBa_B0012 terminator 4218..4258 /label=BBa_B0012 misc_feature 4267..4348 /label=cI lambda promoter 4267..4348 /label=BBa_I9201 misc_feature 4361..4364 /label=conserved RBS 4357..4368 /label=BBa_B0034 CDS 4375..4914 /label=cI tp901 misc_feature 4915..4947 /label=LVA misc_feature 4954..4978 /label=Help:Barcodes CDS 4375..4953 /label=BBa_C0075 stem_loop 4998..5041 /label=stem_loop misc_feature 4987..5066 /label=BBa_B0010 terminator 4987..5066 /label=BBa_B0010 stem_loop 5082..5101 /label=T7 TE misc_feature 5108..5108 /label=stop polyA_site 5102..5115 /label=polya misc_feature 5075..5115 /label=BBa_B0012 terminator 5075..5115 /label=BBa_B0012 misc_feature 5142..5180 /label=cI tp901 OR promoter 5176..5182 /label=-35 misc_feature 5208..5238 /label=cI tp901 OL promoter 5199..5205 /label=-10 misc_feature 5212..5212 /label=start promoter 5124..5240 /label=BBa_R0075 misc_feature 5253..5256 /label=conserved RBS 5249..5260 /label=BBa_B0034 CDS 5267..6028 /label=YFP (LVA) misc_feature 6023..6028 /label=2 misc_feature 5984..6022 /label=SsrA misc_feature 5508..5508 /label=A (Q->L) misc_feature 5267..6028 /label=BBa_E0032 misc_feature 5267..6028 /label=BBa_E0032 stem_loop 6048..6091 /label=stem_loop misc_feature 6037..6116 /label=BBa_B0010 terminator 6037..6116 /label=BBa_B0010 stem_loop 6132..6151 /label=T7 TE misc_feature 6158..6158 /label=stop polyA_site 6152..6165 /label=polya misc_feature 6125..6165 /label=BBa_B0012 terminator 6125..6165 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg 1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg ccacaagttc 1321 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc 1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1501 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc 1681 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc 1741 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 1801 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 1861 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 1921 gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac 1981 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2041 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2101 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttatgac aacttgacgg 2161 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc 2221 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg 2281 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct 2341 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg 2401 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat 2461 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt 2521 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg 2581 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg 2641 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa 2701 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag 2761 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg 2821 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat 2881 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg 2941 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg 3001 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt 3061 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca 3121 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa 3181 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg 3241 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 3301 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agctactaga 3361 gaaagaggag aaatactaga tgagcactaa aaagaaacca cttactcaag agcagttaga 3421 ggacgcccgt cgcttaaaag ccatttatga aaaaaagaaa aatgaattag gcctttccca 3481 ggaatcagtc gccgacaaga tggggatggg gcagtcgggc gttggtgctc tttttaatgg 3541 catcaatgcc cttaatgctt ataacgcggc cctcttagcc aaaattttga aagttagcgt 3601 tgaagaattt agcccttcga tcgcgcgaga aatctacgag atgtatgaag cagttagtat 3661 gcagccgtcg ttacgaagtg agtatgagta ccctgttttt tcacatgttc aggccgggat 3721 gttctcgcct gagttacgaa cctttaccaa aggtgatgca gagcgatggg taagcactac 3781 caaaaaagcg agtgattcag ccttctggtt agaggttgaa ggtaattcca tgaccgcccc 3841 aactggctcc aagccaagct ttcctgacgg aatgcttatt ttggttgacc ctgagcaggc 3901 tgttgagcca ggtgatttct gcatagcgcg attagggggt gatgagttta ccttcaagaa 3961 actgatcagg gatagcggtc aggtgtttct tcaaccacta aacccacagt acccaatgat 4021 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga 4081 gacgtttggc taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa 4141 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 4201 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 4261 ctagagtgca atttagatag tggcgttccc tatttataga ttgtggcagc gacaactgat 4321 aaaatggaga ccgccactat taccaacgta ctagagaaag aggagaaata ctagatgaaa 4381 accgatacca gtaacagatt aaaacaaata atggcggaac gcaacctgaa acaggtggat 4441 atcttaaact tatcgattcc atttcagaaa aaatttggta tcaagctgag taaaagtacg 4501 ctgtcgcagt atgtgaactc tgtgcagtcg ccagaccaga accgcatcta tttactggcg 4561 aaaacgctgg gcgtttccga agcttggctg atgggatttg atgttccaat ggttgaatcg 4621 tcgaaaatcg aaaacgattc ggaaaacatc gaagaaacca ttacggtgat gaaaaaattg 4681 gaagagccga gacagaaagt ggtcctggat accgccaaaa ttcagctgaa agaacaggat 4741 gagcagaaca aagtgaaaca gatcgaagat taccgtctaa gtgatgaata tttagaagaa 4801 cagatcagta aagcgagtgc atatggaggt ggccaattaa atgacaacga taaagaattt 4861 ttcaaacgtt tgttgaaaaa cacgttaaaa gaaaaaattg ataaaggaga tctggctgca 4921 aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta 4981 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 5041 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 5101 ctttctgcgt ttatatacta gagcgcatct tgaacaaaag ttcaacaaaa aaattcatat 5161 ttcgtgaact tttttgttga caaagataaa aacacatgat atactaattt cataaagttc 5221 atgaaacgtg aactgaaatt tactagagaa agaggagaaa tactagatgg tgagcaaggg 5281 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 5341 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 5401 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt 5461 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt 5521 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 5581 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 5641 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 5701 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 5761 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 5821 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca 5881 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 5941 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga 6001 cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg 6061 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 6121 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //