LOCUS BBa_I9120 6148 bp DNA linear 22-Nov-2024 DEFINITION test (luxRI system) R0062.E0432.I0500.E0422.I0500.I9023.I9020 ACCESSION BBa_I9120 VERSION BBa_I9120.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..843 /label=YFP (LVA) misc_feature 838..843 /label=2 misc_feature 799..837 /label=SsrA misc_feature 323..323 /label=A (Q->L) misc_feature 82..843 /label=BBa_E0032 misc_feature 82..843 /label=BBa_E0032 stem_loop 863..906 /label=stem_loop misc_feature 852..931 /label=BBa_B0010 terminator 852..931 /label=BBa_B0010 stem_loop 947..966 /label=T7 TE misc_feature 973..973 /label=stop polyA_site 967..980 /label=polya misc_feature 940..980 /label=BBa_B0012 terminator 940..980 /label=BBa_B0012 promoter 2068..2198 /label=Pbad promoter 2031..2102 /label=PC misc_feature 989..2067 /label=AraC promoter 989..2198 /label=BBa_I0500 misc_feature 2211..2214 /label=conserved RBS 2207..2218 /label=BBa_B0034 CDS 2225..2986 /label=CFP (LVA) misc_feature 2943..2980 /label=SsrA misc_feature 2981..2986 /label=2 misc_feature 2293..2293 /label=A misc_feature 2225..2986 /label=BBa_E0022 misc_feature 2225..2986 /label=BBa_E0022 stem_loop 3006..3049 /label=stem_loop misc_feature 2995..3074 /label=BBa_B0010 terminator 2995..3074 /label=BBa_B0010 stem_loop 3090..3109 /label=T7 TE misc_feature 3116..3116 /label=stop polyA_site 3110..3123 /label=polya misc_feature 3083..3123 /label=BBa_B0012 terminator 3083..3123 /label=BBa_B0012 promoter 4211..4341 /label=Pbad promoter 4174..4245 /label=PC misc_feature 3132..4210 /label=AraC promoter 3132..4341 /label=BBa_I0500 misc_feature 4354..4357 /label=conserved RBS 4350..4361 /label=BBa_B0034 misc_feature 4947..4978 /label=LVA CDS 4368..4946 /label=luxI misc_feature 4368..4985 /label=BBa_C0061 misc_feature 4986..5010 /label=Help:Barcodes CDS 4368..4985 /label=BBa_C0061 stem_loop 5030..5073 /label=stem_loop misc_feature 5019..5098 /label=BBa_B0010 terminator 5019..5098 /label=BBa_B0010 stem_loop 5114..5133 /label=T7 TE misc_feature 5140..5140 /label=stop polyA_site 5134..5147 /label=polya misc_feature 5107..5147 /label=BBa_B0012 terminator 5107..5147 /label=BBa_B0012 promoter 5190..5195 /label=-10 promoter 5170..5175 /label=-35 promoter 5156..5204 /label=BBa_R1075 misc_feature 5217..5220 /label=conserved RBS 5213..5224 /label=BBa_B0034 misc_feature 5231..5232 /label=prefix misc_feature 5404..5404 /label=T misc_feature 5722..5722 /label=A CDS 5231..5980 /label=luxR misc_feature 5231..5986 /label=BBa_C0062 misc_feature 5987..6011 /label=Help:Barcodes CDS 5231..5986 /label=BBa_C0062 stem_loop 6031..6074 /label=stem_loop misc_feature 6020..6099 /label=BBa_B0010 terminator 6020..6099 /label=BBa_B0010 stem_loop 6115..6134 /label=T7 TE misc_feature 6141..6141 /label=stop polyA_site 6135..6148 /label=polya misc_feature 6108..6148 /label=BBa_B0012 terminator 6108..6148 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 961 tgggcctttc tgcgtttata tactagagtt atgacaactt gacggctaca tcattcactt 1021 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc 1081 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc 1141 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga 1201 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat 1261 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac 1321 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc 1381 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc 1441 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat 1501 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt 1561 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac 1621 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt 1681 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt 1741 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta 1801 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt 1861 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca 1921 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc 1981 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa 2041 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct 2101 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac 2161 tctctactgt ttctccatac ccgttttttt gggctagcta ctagagaaag aggagaaata 2221 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 2281 ggacggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 2341 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 2401 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat 2461 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 2521 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 2581 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 2641 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa 2701 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 2761 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 2821 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 2881 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 2941 gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc 3001 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3061 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3121 atatactaga gttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca 3181 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga 3241 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc 3301 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc 3361 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg 3421 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga 3481 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc 3541 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg 3601 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc 3661 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag 3721 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct 3781 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc 3841 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg 3901 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg 3961 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata 4021 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc 4081 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg 4141 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc 4201 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta 4261 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca 4321 tacccgtttt tttgggctag ctactagaga aagaggagaa atactagatg actataatga 4381 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc 4441 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat 4501 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg 4561 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc 4621 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt 4681 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac 4741 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa 4801 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag 4861 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg 4921 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt 4981 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa 5041 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 5101 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttaaa 5161 tttcctcttt tcaggccgga ataactccct ataatgcgcc accatactag agaaagagga 5221 gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa 5281 agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca 5341 ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc 5401 aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa 5461 atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt 5521 tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg 5581 tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag 5641 ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa 5701 cataccatta attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa 5761 taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg 5821 aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca 5881 tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc 5941 aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta 6001 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 6061 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 6121 ccttcgggtg ggcctttctg cgtttata //