LOCUS BBa_I9124 6176 bp DNA linear 22-Nov-2024 DEFINITION test (rhlRI system) R0071.E0432.10500.E0422.I0500.I9027.I9024 ACCESSION BBa_I9124 VERSION BBa_I9124.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=RhlR promoter 19..24 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start promoter 1..53 /label=BBa_R0071 misc_feature 66..69 /label=conserved RBS 62..73 /label=BBa_B0034 CDS 80..841 /label=YFP (LVA) misc_feature 836..841 /label=2 misc_feature 797..835 /label=SsrA misc_feature 321..321 /label=A (Q->L) misc_feature 80..841 /label=BBa_E0032 misc_feature 80..841 /label=BBa_E0032 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 promoter 2066..2196 /label=Pbad promoter 2029..2100 /label=PC misc_feature 987..2065 /label=AraC promoter 987..2196 /label=BBa_I0500 misc_feature 2209..2212 /label=conserved RBS 2205..2216 /label=BBa_B0034 CDS 2223..2984 /label=CFP (LVA) misc_feature 2941..2978 /label=SsrA misc_feature 2979..2984 /label=2 misc_feature 2291..2291 /label=A misc_feature 2223..2984 /label=BBa_E0022 misc_feature 2223..2984 /label=BBa_E0022 stem_loop 3004..3047 /label=stem_loop misc_feature 2993..3072 /label=BBa_B0010 terminator 2993..3072 /label=BBa_B0010 stem_loop 3088..3107 /label=T7 TE misc_feature 3114..3114 /label=stop polyA_site 3108..3121 /label=polya misc_feature 3081..3121 /label=BBa_B0012 terminator 3081..3121 /label=BBa_B0012 promoter 4209..4339 /label=Pbad promoter 4172..4243 /label=PC misc_feature 3130..4208 /label=AraC promoter 3130..4339 /label=BBa_I0500 misc_feature 4352..4355 /label=conserved RBS 4348..4359 /label=BBa_B0034 CDS 4366..4968 /label=rhlL misc_feature 4969..5001 /label=LVA misc_feature 5008..5032 /label=Help:Barcodes CDS 4366..5007 /label=BBa_C0070 stem_loop 5052..5095 /label=stem_loop misc_feature 5041..5120 /label=BBa_B0010 terminator 5041..5120 /label=BBa_B0010 stem_loop 5136..5155 /label=T7 TE misc_feature 5162..5162 /label=stop polyA_site 5156..5169 /label=polya misc_feature 5129..5169 /label=BBa_B0012 terminator 5129..5169 /label=BBa_B0012 promoter 5212..5217 /label=-10 promoter 5192..5197 /label=-35 promoter 5178..5226 /label=BBa_R1075 misc_feature 5239..5242 /label=conserved RBS 5235..5246 /label=BBa_B0034 CDS 5253..5975 /label=rhlR misc_feature 5976..6008 /label=LVA misc_feature 6015..6039 /label=Help:Barcodes CDS 5253..6014 /label=BBa_C0071 stem_loop 6059..6102 /label=stem_loop misc_feature 6048..6127 /label=BBa_B0010 terminator 6048..6127 /label=BBa_B0010 stem_loop 6143..6162 /label=T7 TE misc_feature 6169..6169 /label=stop polyA_site 6163..6176 /label=polya misc_feature 6136..6176 /label=BBa_B0012 terminator 6136..6176 /label=BBa_B0012 ORIGIN 1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga 61 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 241 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg 301 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt 361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 541 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 661 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 781 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata 841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagagttat gacaacttga cggctacatc attcactttt 1021 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc 1081 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg 1141 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg 1201 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc 1261 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa 1321 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc 1381 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct 1441 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc 1501 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag 1561 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg 1621 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct 1681 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc 1741 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa 1801 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca 1861 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga 1921 cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt 1981 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt 2041 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat 2101 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc 2161 tctactgttt ctccataccc gtttttttgg gctagctact agagaaagag gagaaatact 2221 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 2281 acggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 2341 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 2401 ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc gaccacatga 2461 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 2521 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 2581 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 2641 acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga 2701 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 2761 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 2821 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 2881 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga 2941 ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat 3001 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 3061 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 3121 atactagagt tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg 3181 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc 3241 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag 3301 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg 3361 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat 3421 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt 3481 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc 3541 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat 3601 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt 3661 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta 3721 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc 3781 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac 3841 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc 3901 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc 3961 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact 4021 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt 4081 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta 4141 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag 4201 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc 4261 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata 4321 cccgtttttt tgggctagct actagagaaa gaggagaaat actagatgat cgaactgctg 4381 tccgaatccc tggaaggtct gtccgctgct atgatcgctg aactgggtcg ttaccgtcac 4441 caggttttca tcgaaaaact gggttgggac gttgtttcca cctcccgtgt tcgtgaccag 4501 gagttcgacc agttcgacca cccgcagacc cgttacatcg ttgctatgtc ccgtcagggt 4561 atctgcggtt gcgctcgtct gctgccgacc accgacgctt acctgctgaa agacgttttc 4621 gcttacctgt gctccgaaac cccgccgtcc gacccgtccg tttgggaact gtcccgttac 4681 gctgcttccg ctgctgacga cccgcagctg gctatgaaaa tcttctggtc ctccctccag 4741 tgcgcttggt acctgggtgc ttcctccgtt gttgctgtta ccaccaccgc tatggaacgt 4801 tacttcgttc gtaacggtgt tatcctccag cgtctgggtc cgccgcagaa agttaaaggt 4861 gaaaccctgg ttgctatctc cttcccggct taccaggaac gtggtctgga aatgctgctg 4921 cgttaccacc cggaatggct ccagggtgtt ccgctgtcca tggctgttgc tgcaaacgac 4981 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 5041 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 5101 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 5161 gcgtttatat actagagtta aatttcctct tttcaggccg gaataactcc ctataatgcg 5221 ccaccatact agagaaagag gagaaatact agatgaggaa tgacggaggc tttttgctgt 5281 ggtgggacgg tttgcgtagc gagatgcagc cgatccacga cagccagggc gtgttcgccg 5341 tcctggaaaa ggaagtgcgg cgcctgggct tcgattacta cgcctatggc gtgcgccaca 5401 cgattccctt cacccggccg aagaccgagg tccatggcac ctatcccaag gcctggctgg 5461 agcgatacca gatgcagaac tacggggccg tggatccggc gatcctcaac ggcctgcgct 5521 cctcggaaat ggtggtctgg agcgacagcc tgttcgacca gagccggatg ctctggaacg 5581 aggctcgcga ttggggcctc tgtgtcggcg cgaccttgcc gatccgcgcg ccgaacaatt 5641 tgctcagcgt gctttccgtg gcgcgcgacc agcagaacat ctccagcttc gagcgcgagg 5701 aaatccgcct gcggctgcgt tgcatgatcg agttgctgac ccagaagctg accgacctgg 5761 agcatccgat gctgatgtcc aacccggtct gcctgagcca tcgcgaacgc gagatcctgc 5821 aatggaccgc cgacggcaag agttccgggg aaatcgccat catcctgagc atctccgaga 5881 gcacggtgaa cttccaccac aagaacatcc agaagaagtt cgacgcgccg aacaagacgc 5941 tggctgccgc ctacgccgcg gcgctgggtc tcatcgctgc aaacgacgaa aactacgctt 6001 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat 6061 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 6121 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //