BBa_I9124
type: ComponentDefinition
persistent identity: BBa I9124
display id: BBa_I9124
version: 1
was generated by: iGEM to SBOL conversion
description: test (rhlRI system) R0071.E0432.10500.E0422.I0500.I9027.I9024
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: crackdots
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_I0500
component: BBa_B0012
component: BBa_B0010
component: BBa_R0071
component: BBa_E0022
component: BBa_E0032
component: BBa_C0071
component: BBa_R1075
component: BBa_C0070
sequence annotation: BBa_R0071
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_E0022
sequence annotation: BBa_C0070
sequence annotation: BBa_R1075
sequence annotation: BBa_C0071
sequence: https://synbiohub.org/public/igem/BBa_I9124_sequence/1

BBa_B0034
type: Component
persistent identity: component991295
display id: component991295
version: 1
top level: BBa_I9124
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component991290
display id: component991290
version: 1
top level: BBa_I9124
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component991363
display id: component991363
version: 1
top level: BBa_I9124
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component991215
display id: component991215
version: 1
top level: BBa_I9124
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component991353
display id: component991353
version: 1
top level: BBa_I9124
definition: BBa_B0010
access: public

BBa_R0071
type: Component
persistent identity: component991210
display id: component991210
version: 1
top level: BBa_I9124
definition: RhlR+C4
access: public

BBa_B0012
type: Component
persistent identity: component991285
display id: component991285
version: 1
top level: BBa_I9124
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component991275
display id: component991275
version: 1
top level: BBa_I9124
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component991267
display id: component991267
version: 1
top level: BBa_I9124
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component991255
display id: component991255
version: 1
top level: BBa_I9124
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component991250
display id: component991250
version: 1
top level: BBa_I9124
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component991245
display id: component991245
version: 1
top level: BBa_I9124
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component991235
display id: component991235
version: 1
top level: BBa_I9124
definition: BBa_B0010
access: public

BBa_E0032
type: Component
persistent identity: component991227
display id: component991227
version: 1
top level: BBa_I9124
definition: YFP
access: public

BBa_C0071
type: Component
persistent identity: component991347
display id: component991347
version: 1
top level: BBa_I9124
definition: rhlR
access: public

BBa_B0034
type: Component
persistent identity: component991337
display id: component991337
version: 1
top level: BBa_I9124
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component991332
display id: component991332
version: 1
top level: BBa_I9124
definition: const.
access: public

BBa_B0012
type: Component
persistent identity: component991321
display id: component991321
version: 1
top level: BBa_I9124
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component991311
display id: component991311
version: 1
top level: BBa_I9124
definition: BBa_B0010
access: public

BBa_C0070
type: Component
persistent identity: component991305
display id: component991305
version: 1
top level: BBa_I9124
definition: rhII
access: public

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation991210
display id: annotation991210
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991210/range991210/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_I9124/annotation991210/range991210/1
type: Range
persistent identity: range991210
display id: range991210
version: 1
top level: BBa_I9124
direction: direction/forward
start: 1
end: 53
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation991215
display id: annotation991215
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991215/range991215/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9124/annotation991215/range991215/1
type: Range
persistent identity: range991215
display id: range991215
version: 1
top level: BBa_I9124
direction: direction/forward
start: 62
end: 73
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation991227
display id: annotation991227
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991227/range991227/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I9124/annotation991227/range991227/1
type: Range
persistent identity: range991227
display id: range991227
version: 1
top level: BBa_I9124
direction: direction/forward
start: 80
end: 841
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation991235
display id: annotation991235
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991235/range991235/1
component: BBa_B0010

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type: Range
persistent identity: range991235
display id: range991235
version: 1
top level: BBa_I9124
direction: direction/forward
start: 850
end: 929
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation991245
display id: annotation991245
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991245/range991245/1
component: BBa_B0012

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type: Range
persistent identity: range991245
display id: range991245
version: 1
top level: BBa_I9124
direction: direction/forward
start: 938
end: 978
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation991250
display id: annotation991250
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991250/range991250/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9124/annotation991250/range991250/1
type: Range
persistent identity: range991250
display id: range991250
version: 1
top level: BBa_I9124
direction: direction/forward
start: 987
end: 2196
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation991255
display id: annotation991255
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991255/range991255/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9124/annotation991255/range991255/1
type: Range
persistent identity: range991255
display id: range991255
version: 1
top level: BBa_I9124
direction: direction/forward
start: 2205
end: 2216
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation991267
display id: annotation991267
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991267/range991267/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I9124/annotation991267/range991267/1
type: Range
persistent identity: range991267
display id: range991267
version: 1
top level: BBa_I9124
direction: direction/forward
start: 2223
end: 2984
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation991275
display id: annotation991275
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991275/range991275/1
component: BBa_B0010

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type: Range
persistent identity: range991275
display id: range991275
version: 1
top level: BBa_I9124
direction: direction/forward
start: 2993
end: 3072
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation991285
display id: annotation991285
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991285/range991285/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9124/annotation991285/range991285/1
type: Range
persistent identity: range991285
display id: range991285
version: 1
top level: BBa_I9124
direction: direction/forward
start: 3081
end: 3121
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation991290
display id: annotation991290
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991290/range991290/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9124/annotation991290/range991290/1
type: Range
persistent identity: range991290
display id: range991290
version: 1
top level: BBa_I9124
direction: direction/forward
start: 3130
end: 4339
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation991295
display id: annotation991295
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991295/range991295/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9124/annotation991295/range991295/1
type: Range
persistent identity: range991295
display id: range991295
version: 1
top level: BBa_I9124
direction: direction/forward
start: 4348
end: 4359
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation991305
display id: annotation991305
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991305/range991305/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_I9124/annotation991305/range991305/1
type: Range
persistent identity: range991305
display id: range991305
version: 1
top level: BBa_I9124
direction: direction/forward
start: 4366
end: 5007
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation991311
display id: annotation991311
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991311/range991311/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9124/annotation991311/range991311/1
type: Range
persistent identity: range991311
display id: range991311
version: 1
top level: BBa_I9124
direction: direction/forward
start: 5041
end: 5120
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation991321
display id: annotation991321
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991321/range991321/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9124/annotation991321/range991321/1
type: Range
persistent identity: range991321
display id: range991321
version: 1
top level: BBa_I9124
direction: direction/forward
start: 5129
end: 5169
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation991332
display id: annotation991332
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991332/range991332/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I9124/annotation991332/range991332/1
type: Range
persistent identity: range991332
display id: range991332
version: 1
top level: BBa_I9124
direction: direction/forward
start: 5178
end: 5226
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation991337
display id: annotation991337
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991337/range991337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9124/annotation991337/range991337/1
type: Range
persistent identity: range991337
display id: range991337
version: 1
top level: BBa_I9124
direction: direction/forward
start: 5235
end: 5246
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation991347
display id: annotation991347
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991347/range991347/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_I9124/annotation991347/range991347/1
type: Range
persistent identity: range991347
display id: range991347
version: 1
top level: BBa_I9124
direction: direction/forward
start: 5253
end: 6014
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation991353
display id: annotation991353
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991353/range991353/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9124/annotation991353/range991353/1
type: Range
persistent identity: range991353
display id: range991353
version: 1
top level: BBa_I9124
direction: direction/forward
start: 6048
end: 6127
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation991363
display id: annotation991363
version: 1
top level: BBa_I9124
location: https://synbiohub.org/public/igem/BBa_I9124/annotation991363/range991363/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9124/annotation991363/range991363/1
type: Range
persistent identity: range991363
display id: range991363
version: 1
top level: BBa_I9124
direction: direction/forward
start: 6136
end: 6176
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Promoter II
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
top level: const.
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
top level: const.
direction: direction/forward
start: 35
end: 40
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (RhlR & C4-HSL regulated)
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
top level: RhlR+C4
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
top level: RhlR+C4
direction: direction/forward
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
top level: RhlR+C4
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
top level: RhlR+C4
direction: direction/forward
start: 53
end: 53
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: rhlR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
top level: rhlR
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
top level: rhlR
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
top level: rhlR
direction: direction/forward
start: 763
end: 787
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

rhII
type: ComponentDefinition
persistent identity: BBa C0070
display id: BBa_C0070
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3476
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Editing Check (1/28/04) (0) BB sites cleaned (1) ATG (2) TAATAA (3) Blast checks out (i.e., it's RhlI) (4) Codon changes OK (AA and tRNA usage) (5) Chassis might have conflict (w/ native autoinducer system?) (6) ssrA tag added (7) barcode added (8) Codon optimized for expression in enteric bacteria 2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlL
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0070_sequence/1

rhlL
type: SequenceAnnotation
persistent identity: annotation301203
display id: annotation301203
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
type: Range
persistent identity: range301203
display id: range301203
version: 1
top level: rhII
direction: direction/forward
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306820
display id: annotation306820
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
type: Range
persistent identity: range306820
display id: range306820
version: 1
top level: rhII
direction: direction/forward
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213996
display id: annotation2213996
version: 1
top level: rhII
location: https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1
type: Range
persistent identity: range2213996
display id: range2213996
version: 1
top level: rhII
direction: direction/forward
start: 643
end: 667
orientation: inline

https://synbiohub.org/public/igem/BBa_I9124_sequence/1
type: Sequence
persistent identity: BBa I9124 sequence
display id: BBa_I9124_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1075_sequence/1
type: Sequence
persistent identity: BBa R1075 sequence
display id: BBa_R1075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0071_sequence/1
type: Sequence
persistent identity: BBa R0071 sequence
display id: BBa_R0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0070_sequence/1
type: Sequence
persistent identity: BBa C0070 sequence
display id: BBa_C0070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0071_sequence/1
type: Sequence
persistent identity: BBa C0071 sequence
display id: BBa_C0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z