LOCUS BBa_I9128 6394 bp DNA linear 22-Nov-2024 DEFINITION test (cinRI system) R0077.I9045.10500.E0422.I0500.I9031.I9028 ACCESSION BBa_I9128 VERSION BBa_I9128.1 FEATURES Location/Qualifiers RBS 226..231 /label=embedded RBS stem_loop 35..74 /label=stem_loop stem_loop 91..136 /label=stem_loop promoter 1..220 /label=unidentified/uncharacterized CinR dependent promoter 1..231 /label=BBa_R0077 CDS 238..999 /label=YFP (LVA) misc_feature 994..999 /label=2 misc_feature 955..993 /label=SsrA misc_feature 479..479 /label=A (Q->L) misc_feature 238..999 /label=BBa_E0032 misc_feature 238..999 /label=BBa_E0032 stem_loop 1019..1062 /label=stem_loop misc_feature 1008..1087 /label=BBa_B0010 terminator 1008..1087 /label=BBa_B0010 stem_loop 1103..1122 /label=T7 TE misc_feature 1129..1129 /label=stop polyA_site 1123..1136 /label=polya misc_feature 1096..1136 /label=BBa_B0012 terminator 1096..1136 /label=BBa_B0012 promoter 2224..2354 /label=Pbad promoter 2187..2258 /label=PC misc_feature 1145..2223 /label=AraC promoter 1145..2354 /label=BBa_I0500 misc_feature 2367..2370 /label=conserved RBS 2363..2374 /label=BBa_B0034 CDS 2381..3142 /label=CFP (LVA) misc_feature 3099..3136 /label=SsrA misc_feature 3137..3142 /label=2 misc_feature 2449..2449 /label=A misc_feature 2381..3142 /label=BBa_E0022 misc_feature 2381..3142 /label=BBa_E0022 stem_loop 3162..3205 /label=stem_loop misc_feature 3151..3230 /label=BBa_B0010 terminator 3151..3230 /label=BBa_B0010 stem_loop 3246..3265 /label=T7 TE misc_feature 3272..3272 /label=stop polyA_site 3266..3279 /label=polya misc_feature 3239..3279 /label=BBa_B0012 terminator 3239..3279 /label=BBa_B0012 promoter 4367..4497 /label=Pbad promoter 4330..4401 /label=PC misc_feature 3288..4366 /label=AraC promoter 3288..4497 /label=BBa_I0500 misc_feature 4510..4513 /label=conserved RBS 4506..4517 /label=BBa_B0034 CDS 4524..5186 /label=CinI misc_feature 5187..5219 /label=LVA misc_feature 5226..5250 /label=Help:Barcodes CDS 4524..5225 /label=BBa_C0076 stem_loop 5270..5313 /label=stem_loop misc_feature 5259..5338 /label=BBa_B0010 terminator 5259..5338 /label=BBa_B0010 stem_loop 5354..5373 /label=T7 TE misc_feature 5380..5380 /label=stop polyA_site 5374..5387 /label=polya misc_feature 5347..5387 /label=BBa_B0012 terminator 5347..5387 /label=BBa_B0012 promoter 5430..5435 /label=-10 promoter 5410..5415 /label=-35 promoter 5396..5444 /label=BBa_R1075 misc_feature 5457..5460 /label=conserved RBS 5453..5464 /label=BBa_B0034 CDS 5471..6193 /label=cinR misc_feature 6194..6226 /label=LVA misc_feature 6233..6257 /label=Help:Barcodes CDS 5471..6232 /label=BBa_C0077 stem_loop 6277..6320 /label=stem_loop misc_feature 6266..6345 /label=BBa_B0010 terminator 6266..6345 /label=BBa_B0010 stem_loop 6361..6380 /label=T7 TE misc_feature 6387..6387 /label=stop polyA_site 6381..6394 /label=polya misc_feature 6354..6394 /label=BBa_B0012 terminator 6354..6394 /label=BBa_B0012 ORIGIN 1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc 61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac 121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga 181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctggag gtactagatg 241 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 301 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 361 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 421 gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg 481 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 541 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 601 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 661 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 721 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 781 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 841 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 901 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaggcct 961 gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca ggcatcaaat 1021 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 1081 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 1141 agagttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact 1201 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa 1261 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg 1321 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga 1381 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa 1441 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca 1501 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca 1561 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc 1621 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg 1681 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc 1741 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg 1801 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc 1861 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa 1921 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa 1981 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc 2041 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt 2101 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa 2161 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag 2221 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa 2281 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt 2341 ttttttgggc tagctactag agaaagagga gaaatactag atggtgagca agggcgagga 2401 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa 2461 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 2521 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg 2581 gggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 2641 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 2701 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 2761 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat 2821 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa 2881 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 2941 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 3001 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 3061 cgccgggatc actctcggca tggacgagct gtacaagagg cctgctgcaa acgacgaaaa 3121 ctacgcttta gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt 3181 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3241 acactggctc accttcgggt gggcctttct gcgtttatat actagagtta tgacaacttg 3301 acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg 3361 gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg 3421 acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg 3481 tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc 3541 gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg 3601 tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac 3661 tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc 3721 tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa 3781 tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag 3841 ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg 3901 cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca 3961 cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg 4021 agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg 4081 ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc 4141 aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc 4201 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta 4261 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg 4321 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg 4381 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc 4441 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagctac 4501 tagagaaaga ggagaaatac tagatgttcg ttatcatcca ggctcacgaa taccagaaat 4561 acgctgctgt tctggaccag atgttccgtc tgcgtaaaaa agttttcgct gacaccctgt 4621 gctgggacgt tccggttatc ggtccgtacg aacgtgactc ctacgactcc ctggctccgg 4681 cttacctggt ttggtgcaac gactcccgta cccgtctgta cggtggtatg cgtctgatgc 4741 cgaccaccgg tccgaccctg ctgtacgacg ttttccgtga aaccttcccg gacgctgctg 4801 acctgatcgc tccgggtatc tgggaaggta cccgtatgtg catcgacgaa gaagctatcg 4861 ctaaagactt cccggaaatc gacgctggtc gtgctttctc catgatgctg ctggctctgt 4921 gcgaatgcgc tctggaccac ggtatccaca ccatgatctc caactacgaa ccgtacctga 4981 aacgtgttta caaacgtgct ggtgctgaag ttgaagaact gggtcgtgct gacggttacg 5041 gtaaataccc ggtttgctgc ggtgctttcg aagtttccga ccgtgttctg cgtaaaatgc 5101 gtgctgctct gggtctgacc ctgccgctgt acgttcgtca cgttccggct cgttccgttg 5161 ttacccagtt cctggaaatg gctgctgctg ctaacgacga aaactacgct ctggttgctt 5221 aataaccctg atagtgctag tgtagatccc tactagagcc aggcatcaaa taaaacgaaa 5281 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 5341 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttaaa 5401 tttcctcttt tcaggccgga ataactccct ataatgcgcc accatactag agaaagagga 5461 gaaatactag atgattgaga atacctatag cgaaaagttc gagtccgcgt tcgaacagat 5521 caaggcggcg gccaacgtgg atgccgccat ccgtattctc caggcggaat ataacctcga 5581 tttcgtcacc taccatctcg cccagacgat cgcgagcaag atcgattcgc ccttcgtgcg 5641 caccacctat ccggatgcct gggtttcccg ctacctcctc aacagctatg tgaaggtcga 5701 tccgatcgtc aagcagggct tcgaacgcca gctgcccttc gactggagcg aggtcgaacc 5761 gacgccggag gcctatgcca tgctggtcga cgcccagaaa cacggcatcg gtggcaatgg 5821 ctactccatc cccgtcgccg acaaggcgca gcgccgcgcc ctgctgtcgc tgaatgcccg 5881 tataccggcc gacgaatgga ccgagctcgt gcgccgctgc cgcaacgagt ggatcgagat 5941 cgcccatctg atccaccgca aggccgtcta tgagctgcat ggcgaaaacg atccggtgcc 6001 ggcattgtcg ccgcgcgaga tcgagtgtct gcactggacc gccctcggca aggattacaa 6061 ggatatttcg gtcatcctgg gcatatcaga gcataccaca cgcgattacc tgaagaccgc 6121 ccgcttcaag ctcggctgcg ccacgatctc ggccgccgcg tcgcgggctg ttcaattgcg 6181 catcatcaat cccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata 6241 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 6301 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 6361 ggctcacctt cgggtgggcc tttctgcgtt tata //