BBa_I9128
type: ComponentDefinition
persistent identity: BBa I9128
display id: BBa_I9128
version: 1
was generated by: iGEM to SBOL conversion
description: test (cinRI system) R0077.I9045.10500.E0422.I0500.I9031.I9028
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: crackdots
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_I0500
component: BBa_B0012
component: BBa_B0010
component: BBa_E0032
component: BBa_R0077
component: BBa_C0077
component: BBa_B0034
component: BBa_R1075
component: BBa_C0076
component: BBa_E0022
sequence annotation: BBa_R0077
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_C0076
sequence annotation: BBa_R1075
sequence annotation: BBa_C0077
sequence: https://synbiohub.org/public/igem/BBa_I9128_sequence/1

BBa_I0500
type: Component
persistent identity: component988638
display id: component988638
version: 1
top level: BBa_I9128
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component988633
display id: component988633
version: 1
top level: BBa_I9128
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component988623
display id: component988623
version: 1
top level: BBa_I9128
definition: BBa_B0010
access: public

BBa_E0032
type: Component
persistent identity: component988615
display id: component988615
version: 1
top level: BBa_I9128
definition: YFP
access: public

BBa_R0077
type: Component
persistent identity: component988603
display id: component988603
version: 1
top level: BBa_I9128
definition: cinR
access: public

BBa_B0012
type: Component
persistent identity: component988751
display id: component988751
version: 1
top level: BBa_I9128
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component988741
display id: component988741
version: 1
top level: BBa_I9128
definition: BBa_B0010
access: public

BBa_C0077
type: Component
persistent identity: component988735
display id: component988735
version: 1
top level: BBa_I9128
definition: cinr
access: public

BBa_B0034
type: Component
persistent identity: component988725
display id: component988725
version: 1
top level: BBa_I9128
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component988720
display id: component988720
version: 1
top level: BBa_I9128
definition: const.
access: public

BBa_B0012
type: Component
persistent identity: component988709
display id: component988709
version: 1
top level: BBa_I9128
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component988699
display id: component988699
version: 1
top level: BBa_I9128
definition: BBa_B0010
access: public

BBa_C0076
type: Component
persistent identity: component988693
display id: component988693
version: 1
top level: BBa_I9128
definition: cinI
access: public

BBa_B0034
type: Component
persistent identity: component988683
display id: component988683
version: 1
top level: BBa_I9128
definition: BBa_B0034
access: public

BBa_I0500
type: Component
persistent identity: component988678
display id: component988678
version: 1
top level: BBa_I9128
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component988673
display id: component988673
version: 1
top level: BBa_I9128
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component988663
display id: component988663
version: 1
top level: BBa_I9128
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component988655
display id: component988655
version: 1
top level: BBa_I9128
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component988643
display id: component988643
version: 1
top level: BBa_I9128
definition: BBa_B0034
access: public

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation988603
display id: annotation988603
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988603/range988603/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_I9128/annotation988603/range988603/1
type: Range
persistent identity: range988603
display id: range988603
version: 1
top level: BBa_I9128
direction: direction/forward
start: 1
end: 231
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation988615
display id: annotation988615
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988615/range988615/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I9128/annotation988615/range988615/1
type: Range
persistent identity: range988615
display id: range988615
version: 1
top level: BBa_I9128
direction: direction/forward
start: 238
end: 999
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation988623
display id: annotation988623
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988623/range988623/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9128/annotation988623/range988623/1
type: Range
persistent identity: range988623
display id: range988623
version: 1
top level: BBa_I9128
direction: direction/forward
start: 1008
end: 1087
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation988633
display id: annotation988633
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988633/range988633/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9128/annotation988633/range988633/1
type: Range
persistent identity: range988633
display id: range988633
version: 1
top level: BBa_I9128
direction: direction/forward
start: 1096
end: 1136
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation988638
display id: annotation988638
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988638/range988638/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9128/annotation988638/range988638/1
type: Range
persistent identity: range988638
display id: range988638
version: 1
top level: BBa_I9128
direction: direction/forward
start: 1145
end: 2354
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation988643
display id: annotation988643
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988643/range988643/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9128/annotation988643/range988643/1
type: Range
persistent identity: range988643
display id: range988643
version: 1
top level: BBa_I9128
direction: direction/forward
start: 2363
end: 2374
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation988655
display id: annotation988655
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988655/range988655/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I9128/annotation988655/range988655/1
type: Range
persistent identity: range988655
display id: range988655
version: 1
top level: BBa_I9128
direction: direction/forward
start: 2381
end: 3142
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation988663
display id: annotation988663
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988663/range988663/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9128/annotation988663/range988663/1
type: Range
persistent identity: range988663
display id: range988663
version: 1
top level: BBa_I9128
direction: direction/forward
start: 3151
end: 3230
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation988673
display id: annotation988673
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988673/range988673/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9128/annotation988673/range988673/1
type: Range
persistent identity: range988673
display id: range988673
version: 1
top level: BBa_I9128
direction: direction/forward
start: 3239
end: 3279
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation988678
display id: annotation988678
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988678/range988678/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I9128/annotation988678/range988678/1
type: Range
persistent identity: range988678
display id: range988678
version: 1
top level: BBa_I9128
direction: direction/forward
start: 3288
end: 4497
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation988683
display id: annotation988683
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988683/range988683/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9128/annotation988683/range988683/1
type: Range
persistent identity: range988683
display id: range988683
version: 1
top level: BBa_I9128
direction: direction/forward
start: 4506
end: 4517
orientation: inline

BBa_C0076
type: SequenceAnnotation
persistent identity: annotation988693
display id: annotation988693
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988693/range988693/1
component: BBa_C0076

https://synbiohub.org/public/igem/BBa_I9128/annotation988693/range988693/1
type: Range
persistent identity: range988693
display id: range988693
version: 1
top level: BBa_I9128
direction: direction/forward
start: 4524
end: 5225
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation988699
display id: annotation988699
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988699/range988699/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9128/annotation988699/range988699/1
type: Range
persistent identity: range988699
display id: range988699
version: 1
top level: BBa_I9128
direction: direction/forward
start: 5259
end: 5338
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation988709
display id: annotation988709
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988709/range988709/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9128/annotation988709/range988709/1
type: Range
persistent identity: range988709
display id: range988709
version: 1
top level: BBa_I9128
direction: direction/forward
start: 5347
end: 5387
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation988720
display id: annotation988720
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988720/range988720/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I9128/annotation988720/range988720/1
type: Range
persistent identity: range988720
display id: range988720
version: 1
top level: BBa_I9128
direction: direction/forward
start: 5396
end: 5444
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation988725
display id: annotation988725
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988725/range988725/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9128/annotation988725/range988725/1
type: Range
persistent identity: range988725
display id: range988725
version: 1
top level: BBa_I9128
direction: direction/forward
start: 5453
end: 5464
orientation: inline

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation988735
display id: annotation988735
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988735/range988735/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_I9128/annotation988735/range988735/1
type: Range
persistent identity: range988735
display id: range988735
version: 1
top level: BBa_I9128
direction: direction/forward
start: 5471
end: 6232
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation988741
display id: annotation988741
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988741/range988741/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I9128/annotation988741/range988741/1
type: Range
persistent identity: range988741
display id: range988741
version: 1
top level: BBa_I9128
direction: direction/forward
start: 6266
end: 6345
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation988751
display id: annotation988751
version: 1
top level: BBa_I9128
location: https://synbiohub.org/public/igem/BBa_I9128/annotation988751/range988751/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I9128/annotation988751/range988751/1
type: Range
persistent identity: range988751
display id: range988751
version: 1
top level: BBa_I9128
direction: direction/forward
start: 6354
end: 6394
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Promoter II
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
top level: const.
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
top level: const.
direction: direction/forward
start: 35
end: 40
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

cinI
type: ComponentDefinition
persistent identity: BBa C0076
display id: BBa_C0076
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cinI codes for an autoinducer synthetase which utilizes SAM to make O3-C14:1-HSL. In complex with O3-C14:1-HSL, CinR (BBa_C0077) binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tga -> tAaTAA
star: true
creator: crackdots
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CinI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0076_sequence/1

CinI
type: SequenceAnnotation
persistent identity: annotation301035
display id: annotation301035
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
type: Range
persistent identity: range301035
display id: range301035
version: 1
top level: cinI
direction: direction/forward
start: 1
end: 663
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306586
display id: annotation306586
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
type: Range
persistent identity: range306586
display id: range306586
version: 1
top level: cinI
direction: direction/forward
start: 664
end: 696
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214001
display id: annotation2214001
version: 1
top level: cinI
location: https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
type: Range
persistent identity: range2214001
display id: range2214001
version: 1
top level: cinI
direction: direction/forward
start: 703
end: 727
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: iGEM to SBOL conversion
description: cinR activator from Rhizobium leguminosarum (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Rhizobium leguminosarum
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
star: true
creator: crackdots
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cinR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
top level: cinr
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
top level: cinr
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
top level: cinr
direction: direction/forward
start: 763
end: 787
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0077
display id: BBa_R0077
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (cinR and HSL regulated, RBS+)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription. This promoter contains the RBS: TGGAGG
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
star: true
creator: crackdots
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence annotation: embedded RBS
sequence: https://synbiohub.org/public/igem/BBa_R0077_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation301371
display id: annotation301371
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1
type: Range
persistent identity: range301371
display id: range301371
version: 1
top level: cinR
direction: direction/forward
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301204
display id: annotation301204
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1
type: Range
persistent identity: range301204
display id: range301204
version: 1
top level: cinR
direction: direction/forward
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301205
display id: annotation301205
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1
type: Range
persistent identity: range301205
display id: range301205
version: 1
top level: cinR
direction: direction/forward
start: 91
end: 136
orientation: inline

embedded RBS
type: SequenceAnnotation
persistent identity: annotation301150
display id: annotation301150
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1
type: Range
persistent identity: range301150
display id: range301150
version: 1
top level: cinR
direction: direction/forward
start: 226
end: 231
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

https://synbiohub.org/public/igem/BBa_I9128_sequence/1
type: Sequence
persistent identity: BBa I9128 sequence
display id: BBa_I9128_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggaggtactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1075_sequence/1
type: Sequence
persistent identity: BBa R1075 sequence
display id: BBa_R1075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0076_sequence/1
type: Sequence
persistent identity: BBa C0076 sequence
display id: BBa_C0076_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0077_sequence/1
type: Sequence
persistent identity: BBa C0077 sequence
display id: BBa_C0077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgagaatacctatagcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0077_sequence/1
type: Sequence
persistent identity: BBa R0077 sequence
display id: BBa_R0077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctggagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z