LOCUS       BBa_J00000              3222 bp    DNA     linear       22-Dec-2024
DEFINITION  base metals -> gold
ACCESSION   BBa_J00000
VERSION     BBa_J00000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     stem_loop       70..89
                     /label=T7 TE
     misc_feature    96..96
                     /label=stop
     polyA_site      90..103
                     /label=polya
     misc_feature    63..103
                     /label=BBa_B0012
     terminator      63..103
                     /label=BBa_B0012
     CDS             140..445
                     /label=ccdB (rev)
     CDS             447..667
                     /label=ccdA- (rev)
     misc_feature    578..578
                     /label=T to C mutation
     misc_feature    706..711
                     /label=-10 (rev)
     misc_feature    729..734
                     /label=-35 (rev)
     misc_feature    112..786
                     /label=BBa_P1010
     promoter        1874..2004
                     /label=Pbad
     promoter        1837..1908
                     /label=PC
     misc_feature    795..1873
                     /label=AraC
     promoter        795..2004
                     /label=BBa_I0500
     promoter        3092..3222
                     /label=Pbad
     promoter        3055..3126
                     /label=PC
     misc_feature    2013..3091
                     /label=AraC
     promoter        2013..3222
                     /label=BBa_I0500
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gactggctgt
      121 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
      181 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg
      241 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
      301 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
      361 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
      421 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
      481 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
      541 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
      601 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
      661 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
      721 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
      781 acgcgttact agagttatga caacttgacg gctacatcat tcactttttc ttcacaaccg
      841 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt
      901 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa
      961 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac
     1021 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg
     1081 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc
     1141 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat
     1201 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta
     1261 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac
     1321 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga
     1381 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc
     1441 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt
     1501 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt
     1561 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga
     1621 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc
     1681 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac
     1741 tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt
     1801 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac
     1861 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt
     1921 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     1981 ccatacccgt ttttttgggc tagctactag agttatgaca acttgacggc tacatcattc
     2041 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
     2101 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
     2161 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
     2221 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
     2281 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
     2341 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
     2401 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
     2461 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
     2521 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
     2581 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
     2641 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
     2701 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
     2761 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
     2821 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
     2881 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
     2941 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc
     3001 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
     3061 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
     3121 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
     3181 caactctcta ctgtttctcc atacccgttt ttttgggcta gc
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