##gff-version 3
##sequence-region BBa_J00000 1 3222
BBa_J00000	.	promoter	2013	3222	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_J00000	.	promoter	3092	3222	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J00000	.	sequence_feature	2013	3091	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J00000	.	promoter	3055	3126	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J00000	.	engineered_region	112	786	.	+	0	ID=BBa_P1010;Name=BBa_P1010
BBa_J00000	.	point_mutation	578	578	.	+	0	ID=annotation1747334;Name=T to C mutation;Parent=BBa_P1010
BBa_J00000	.	sequence_feature	706	711	.	+	0	ID=annotation1747335;Name=-10 (rev);Parent=BBa_P1010
BBa_J00000	.	sequence_feature	729	734	.	+	0	ID=annotation1747336;Name=-35 (rev);Parent=BBa_P1010
BBa_J00000	.	CDS	140	445	.	+	0	ID=annotation1747332;Name=ccdB (rev);Parent=BBa_P1010
BBa_J00000	.	CDS	447	667	.	+	0	ID=annotation1747333;Name=ccdA- (rev);Parent=BBa_P1010
BBa_J00000	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J00000	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J00000	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J00000	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J00000	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J00000	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J00000	.	promoter	795	2004	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_J00000	.	promoter	1874	2004	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J00000	.	sequence_feature	795	1873	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J00000	.	promoter	1837	1908	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J00000	.	terminator	63	103	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J00000	.	stem_loop	70	89	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J00000	.	polyA_site	90	103	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J00000	.	stop_codon	96	96	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J00000	.	engineered_region	63	103	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_J00000
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagactggctgtgtataagggagcctgacatttatattccccagaacatc
aggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacac
tggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatc
tgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccaca
aacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaa
gagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagac
gacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctg
tcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtata
tattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgttact
agagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggt
gcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgg
gtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggc
ggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccag
gtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatg
cgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatga
tttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacgg
ccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatga
cgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttt
tcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgag
ataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattt
tgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtc
actgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggac
caaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtc
acactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactg
tttctccatacccgtttttttgggctagctactagagttatgacaacttgacggctacatcattcactttttcttcaca
accggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaacca
acattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgc
gccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgc
gacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatc
ggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccg
aatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgg
gcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacc
cactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcac
ccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataaccttt
cattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggca
ttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaa
ccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaacccc
gcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggc
agaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatc
ctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc