BBa_J01020
1
BBa_J01020
OnKey1 = [Key promoter KP3][key1]
2005-11-05T12:00:00Z
2015-08-31T04:08:12Z
[Key promoter KP3][key1]
false
false
_13_
0
395
13
Not in stock
false
false
Golden Bear
component1775522
1
BBa_J01006
component1775524
1
BBa_J01008
annotation1775522
1
BBa_J01006
range1775522
1
1
59
annotation1775524
1
BBa_J01008
range1775524
1
68
161
BBa_J01008
1
Key 1
Riboregulator key 1
2005-11-05T12:00:00Z
2015-08-31T04:08:12Z
Released HQ 2013
Biobricked version of Isaacs' riboregulator trans activating key, taR12
false
true
_13_
0
395
13
In stock
false
false
Golden Bear
BBa_J01006
1
KP3
Key Promoter absorbs 3
2005-10-31T12:00:00Z
2015-08-31T04:08:11Z
Promoter for transcribing keys to act on locks based on Isaacs, Collins, et. al: <a href="http://www.nature.com/nbt/journal/v22/n7/full/nbt986.html;jsessionid=464FF968289CFEAE2031D9BC81CDF6EC">
"Engineered riboregulators enable post-transcriptional control of gene expression"</a>
KP3 absorbs three nucleotides from mixed site ("TACTAGAG"), so that the key has a 5 nucleotide spacer region (i.e. "TAGAG") between the transcription start site and the first nucleotide of the key.
We are still looking into what the optimal size for the spacer region is. See also KP2 (BBa_J01007), and Key1 (BBa_J01008), Key2 (BBa_J01009), Lock1 (BBa_J01010), and Lock2 (BBa_J01011).
false
false
_13_
0
395
13
Not in stock
false
false
Golden Bear
BBa_J01020_sequence
1
tcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccatactagagacccaaatccaggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
BBa_J01008_sequence
1
acccaaatccaggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
BBa_J01006_sequence
1
tcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgcca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z