LOCUS       BBa_J01022              1060 bp    DNA     linear       16-May-2024
DEFINITION  [DblTerminator][OnRFP]
ACCESSION   BBa_J01022
VERSION     BBa_J01022.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..156
                     /label=TetR 1
     misc_feature    138..191
                     /label=BBa_R0040
     promoter        157..162
                     /label=-35
     misc_feature    163..181
                     /label=TetR 2
     promoter        180..185
                     /label=-10
     promoter        138..191
                     /label=BBa_R0040
     misc_feature    204..207
                     /label=conserved
     RBS             200..211
                     /label=BBa_B0034
     CDS             218..892
                     /label=mrfp1
     misc_feature    899..923
                     /label=Help:Barcodes
     CDS             218..898
                     /label=BBa_E1010
     stem_loop       943..986
                     /label=stem_loop
     misc_feature    932..1011
                     /label=BBa_B0010
     terminator      932..1011
                     /label=BBa_B0010
     stem_loop       1027..1046
                     /label=T7 TE
     misc_feature    1053..1053
                     /label=stop
     polyA_site      1047..1060
                     /label=polya
     misc_feature    1020..1060
                     /label=BBa_B0012
     terminator      1020..1060
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
      181 atactgagca ctactagaga aagaggagaa atactagatg gcttcctccg aagacgttat
      241 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
      301 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
      361 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
      421 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
      481 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
      541 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
      601 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
      661 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
      721 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
      781 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
      841 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
      901 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
      961 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1021 cacactggct caccttcggg tgggcctttc tgcgtttata
//