LOCUS       BBa_J01025              1723 bp    DNA     linear       25-Nov-2024
DEFINITION  Non mobilizable TraJR, OnRFP, Key1 = [pRM TraJR][DblTerminator][OnR
            FP][OnKey1]
ACCESSION   BBa_J01025
VERSION     BBa_J01025.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             109..486
                     /label=TraJr
     CDS             109..486
                     /label=BBa_J01001
     misc_feature    91..486
                     /label=BBa_J01021
     misc_feature    1..486
                     /label=BBa_J01023
     stem_loop       506..549
                     /label=stem_loop
     misc_feature    495..574
                     /label=BBa_B0010
     terminator      495..574
                     /label=BBa_B0010
     stem_loop       590..609
                     /label=T7 TE
     misc_feature    616..616
                     /label=stop
     polyA_site      610..623
                     /label=polya
     misc_feature    583..623
                     /label=BBa_B0012
     terminator      583..623
                     /label=BBa_B0012
     misc_feature    632..650
                     /label=TetR 1
     misc_feature    632..685
                     /label=BBa_R0040
     promoter        651..656
                     /label=-35
     misc_feature    657..675
                     /label=TetR 2
     promoter        674..679
                     /label=-10
     promoter        632..685
                     /label=BBa_R0040
     misc_feature    698..701
                     /label=conserved
     RBS             694..705
                     /label=BBa_B0034
     CDS             712..1386
                     /label=mrfp1
     misc_feature    1393..1417
                     /label=Help:Barcodes
     CDS             712..1392
                     /label=BBa_E1010
     stem_loop       1437..1480
                     /label=stem_loop
     misc_feature    1426..1505
                     /label=BBa_B0010
     terminator      1426..1505
                     /label=BBa_B0010
     stem_loop       1521..1540
                     /label=T7 TE
     misc_feature    1547..1547
                     /label=stop
     polyA_site      1541..1554
                     /label=polya
     misc_feature    1514..1554
                     /label=BBa_B0012
     terminator      1514..1554
                     /label=BBa_B0012
     misc_feature    495..1554
                     /label=BBa_J01022
     misc_feature    1..1554
                     /label=BBa_J01024
     promoter        1563..1621
                     /label=BBa_J01006
     misc_feature    1630..1723
                     /label=BBa_J01008
     misc_feature    1563..1723
                     /label=BBa_J01020
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag aaagaggaga aatactagat ggctgatgaa
      121 accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc agacgaacga
      181 agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc
      241 gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg cgagctggcc
      301 cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct caccgacgac
      361 ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa gatcgaagag
      421 aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc agagccatga
      481 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      541 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      601 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc
      661 tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta gatggcttcc
      721 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      781 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      841 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      901 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      961 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
     1021 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
     1081 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
     1141 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
     1201 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1261 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1321 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1381 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
     1441 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1501 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1561 agtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
     1621 atactagaga cccaaatcca ggaggtgaat ctagtaggtg gttaatgaaa attaacttac
     1681 ttactagaaa tatctctaaa aagccagatt attaatccgg ctt
//