##gff-version 3
##sequence-region BBa_J01025 1 1723
BBa_J01025	.	engineered_region	1563	1723	.	+	0	ID=BBa_J01020;Name=BBa_J01020
BBa_J01025	.	promoter	1563	1621	.	+	0	ID=BBa_J01006;Name=BBa_J01006;Parent=BBa_J01020
BBa_J01025	.	mature_transcript_region	1630	1723	.	+	0	ID=BBa_J01008;Name=BBa_J01008;Parent=BBa_J01020
BBa_J01025	.	engineered_region	1	1554	.	+	0	ID=BBa_J01024;Name=BBa_J01024
BBa_J01025	.	engineered_region	495	1554	.	+	0	ID=BBa_J01022;Name=BBa_J01022;Parent=BBa_J01024
BBa_J01025	.	terminator	1426	1505	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01022
BBa_J01025	.	stem_loop	1437	1480	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01025	.	engineered_region	1426	1505	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01025	.	promoter	632	685	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J01022
BBa_J01025	.	engineered_region	632	685	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J01025	.	promoter	674	679	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J01025	.	non_covalent_binding_site	657	675	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J01025	.	promoter	651	656	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J01025	.	non_covalent_binding_site	632	650	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J01025	.	terminator	1514	1554	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01022
BBa_J01025	.	stop_codon	1547	1547	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01025	.	polyA_site	1541	1554	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01025	.	stem_loop	1521	1540	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01025	.	engineered_region	1514	1554	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01025	.	terminator	583	623	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01022
BBa_J01025	.	stop_codon	616	616	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01025	.	polyA_site	610	623	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01025	.	stem_loop	590	609	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01025	.	engineered_region	583	623	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01025	.	CDS	712	1392	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J01022
BBa_J01025	.	CDS	712	1386	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J01025	.	engineered_tag	1393	1417	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J01025	.	terminator	495	574	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01022
BBa_J01025	.	stem_loop	506	549	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01025	.	engineered_region	495	574	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01025	.	ribosome_entry_site	694	705	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J01022
BBa_J01025	.	conserved_region	698	701	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J01025	.	engineered_region	1	486	.	+	0	ID=BBa_J01023;Name=BBa_J01023;Parent=BBa_J01024
BBa_J01025	.	engineered_region	91	486	.	+	0	ID=BBa_J01021;Name=BBa_J01021;Parent=BBa_J01023
BBa_J01025	.	ribosome_entry_site	91	102	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J01021
BBa_J01025	.	conserved_region	95	98	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J01025	.	CDS	109	486	.	+	0	ID=BBa_J01001;Name=BBa_J01001;Parent=BBa_J01021
BBa_J01025	.	CDS	109	486	.	+	0	ID=annotation1891611;Name=TraJr;Parent=BBa_J01001
BBa_J01025	.	promoter	1	82	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_J01023
BBa_J01025	.	promoter	48	53	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_J01025	.	sequence_alteration	59	65	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_J01025	.	non_covalent_binding_site	9	25	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_J01025	.	non_covalent_binding_site	33	49	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_J01025	.	promoter	71	76	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
>BBa_J01025
gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaa
cgttactagagaaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggt
gtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctg
gccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacc
tgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcct
cgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagag
ccatgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagt
ccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactag
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagt
tcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtcc
gctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatc
ccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtta
ccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccga
cggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa
ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaa
aaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgt
tgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcagaaaattattttaaa
tttcctcttgtcaggccggaataactccctataatgcgccatactagagacccaaatccaggaggtgaatctagtaggt
ggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt