TraJR Nonm
type: ComponentDefinition
persistent identity: BBa J01025
display id: BBa_J01025
version: 1
was generated by: iGEM to SBOL conversion
description: Non mobilizable TraJR, OnRFP, Key1 = [pRM TraJR][DblTerminator][OnRFP][OnKey1]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J01024
component: BBa_J01020
sequence annotation: BBa_J01024
sequence annotation: BBa_J01020
sequence: https://synbiohub.org/public/igem/BBa_J01025_sequence/1

BBa_J01024
type: Component
persistent identity: component2232582
display id: component2232582
version: 1
top level: TraJR Nonm
definition: BBa_J01024
access: public

BBa_J01020
type: Component
persistent identity: component2232585
display id: component2232585
version: 1
top level: TraJR Nonm
definition: BBa_J01020
access: public

BBa_J01024
type: SequenceAnnotation
persistent identity: annotation2232582
display id: annotation2232582
version: 1
top level: TraJR Nonm
location: https://synbiohub.org/public/igem/BBa_J01025/annotation2232582/range2232582/1
component: BBa_J01024

https://synbiohub.org/public/igem/BBa_J01025/annotation2232582/range2232582/1
type: Range
persistent identity: range2232582
display id: range2232582
version: 1
top level: TraJR Nonm
direction: direction/reverse
start: 1
end: 1554
orientation: inline

BBa_J01020
type: SequenceAnnotation
persistent identity: annotation2232585
display id: annotation2232585
version: 1
top level: TraJR Nonm
location: https://synbiohub.org/public/igem/BBa_J01025/annotation2232585/range2232585/1
component: BBa_J01020

https://synbiohub.org/public/igem/BBa_J01025/annotation2232585/range2232585/1
type: Range
persistent identity: range2232585
display id: range2232585
version: 1
top level: TraJR Nonm
direction: direction/reverse
start: 1563
end: 1723
orientation: inline

BBa_J01020
type: ComponentDefinition
persistent identity: BBa J01020
display id: BBa_J01020
version: 1
was generated by: iGEM to SBOL conversion
description: OnKey1 = [Key promoter KP3][key1]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: [Key promoter KP3][key1]
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J01006
component: BBa_J01008
sequence annotation: BBa_J01006
sequence annotation: BBa_J01008
sequence: https://synbiohub.org/public/igem/BBa_J01020_sequence/1

BBa_J01006
type: Component
persistent identity: component1775522
display id: component1775522
version: 1
top level: BBa_J01020
definition: KP3
access: public

BBa_J01008
type: Component
persistent identity: component1775524
display id: component1775524
version: 1
top level: BBa_J01020
definition: Key 1
access: public

BBa_J01006
type: SequenceAnnotation
persistent identity: annotation1775522
display id: annotation1775522
version: 1
top level: BBa_J01020
location: https://synbiohub.org/public/igem/BBa_J01020/annotation1775522/range1775522/1
component: BBa_J01006

https://synbiohub.org/public/igem/BBa_J01020/annotation1775522/range1775522/1
type: Range
persistent identity: range1775522
display id: range1775522
version: 1
top level: BBa_J01020
direction: direction/forward
start: 1
end: 59
orientation: inline

BBa_J01008
type: SequenceAnnotation
persistent identity: annotation1775524
display id: annotation1775524
version: 1
top level: BBa_J01020
location: https://synbiohub.org/public/igem/BBa_J01020/annotation1775524/range1775524/1
component: BBa_J01008

https://synbiohub.org/public/igem/BBa_J01020/annotation1775524/range1775524/1
type: Range
persistent identity: range1775524
display id: range1775524
version: 1
top level: BBa_J01020
direction: direction/forward
start: 68
end: 161
orientation: inline

BBa_J01024
type: ComponentDefinition
persistent identity: BBa J01024
display id: BBa_J01024
version: 1
was generated by: iGEM to SBOL conversion
description: [pRM TraJR][DblTerminator][OnRFP]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J01022
component: BBa_J01023
sequence annotation: BBa_J01023
sequence annotation: BBa_J01022
sequence: https://synbiohub.org/public/igem/BBa_J01024_sequence/1

BBa_J01022
type: Component
persistent identity: component2230047
display id: component2230047
version: 1
top level: BBa_J01024
definition: T.OnRFP
access: public

BBa_J01023
type: Component
persistent identity: component2230024
display id: component2230024
version: 1
top level: BBa_J01024
definition: BBa_J01023
access: public

BBa_J01023
type: SequenceAnnotation
persistent identity: annotation2230024
display id: annotation2230024
version: 1
top level: BBa_J01024
location: https://synbiohub.org/public/igem/BBa_J01024/annotation2230024/range2230024/1
component: BBa_J01023

https://synbiohub.org/public/igem/BBa_J01024/annotation2230024/range2230024/1
type: Range
persistent identity: range2230024
display id: range2230024
version: 1
top level: BBa_J01024
direction: direction/reverse
start: 1
end: 486
orientation: inline

BBa_J01022
type: SequenceAnnotation
persistent identity: annotation2230047
display id: annotation2230047
version: 1
top level: BBa_J01024
location: https://synbiohub.org/public/igem/BBa_J01024/annotation2230047/range2230047/1
component: BBa_J01022

https://synbiohub.org/public/igem/BBa_J01024/annotation2230047/range2230047/1
type: Range
persistent identity: range2230047
display id: range2230047
version: 1
top level: BBa_J01024
direction: direction/reverse
start: 495
end: 1554
orientation: inline

KP3
type: ComponentDefinition
persistent identity: BBa J01006
display id: BBa_J01006
version: 1
was generated by: iGEM to SBOL conversion
description: Key Promoter absorbs 3
created: 2005-10-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
mutable description: Promoter for transcribing keys to act on locks based on Isaacs, Collins, et. al: "Engineered riboregulators enable post-transcriptional control of gene expression" KP3 absorbs three nucleotides from mixed site ("TACTAGAG"), so that the key has a 5 nucleotide spacer region (i.e. "TAGAG") between the transcription start site and the first nucleotide of the key. We are still looking into what the optimal size for the spacer region is. See also KP2 (BBa_J01007), and Key1 (BBa_J01008), Key2 (BBa_J01009), Lock1 (BBa_J01010), and Lock2 (BBa_J01011).
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J01006_sequence/1

T.OnRFP
type: ComponentDefinition
persistent identity: BBa J01022
display id: BBa_J01022
version: 1
was generated by: iGEM to SBOL conversion
description: [DblTerminator][OnRFP]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: [DblTerminator][OnRFP]
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_J01022_sequence/1

BBa_B0010
type: Component
persistent identity: component1737854
display id: component1737854
version: 1
top level: T.OnRFP
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1737848
display id: component1737848
version: 1
top level: T.OnRFP
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1737864
display id: component1737864
version: 1
top level: T.OnRFP
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component1737818
display id: component1737818
version: 1
top level: T.OnRFP
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1737808
display id: component1737808
version: 1
top level: T.OnRFP
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1737841
display id: component1737841
version: 1
top level: T.OnRFP
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1737833
display id: component1737833
version: 1
top level: T.OnRFP
definition: p(tetR)
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1737808
display id: annotation1737808
version: 1
top level: T.OnRFP
location: https://synbiohub.org/public/igem/BBa_J01022/annotation1737808/range1737808/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J01022/annotation1737808/range1737808/1
type: Range
persistent identity: range1737808
display id: range1737808
version: 1
top level: T.OnRFP
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1737818
display id: annotation1737818
version: 1
top level: T.OnRFP
location: https://synbiohub.org/public/igem/BBa_J01022/annotation1737818/range1737818/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J01022/annotation1737818/range1737818/1
type: Range
persistent identity: range1737818
display id: range1737818
version: 1
top level: T.OnRFP
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1737833
display id: annotation1737833
version: 1
top level: T.OnRFP
location: https://synbiohub.org/public/igem/BBa_J01022/annotation1737833/range1737833/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J01022/annotation1737833/range1737833/1
type: Range
persistent identity: range1737833
display id: range1737833
version: 1
top level: T.OnRFP
direction: direction/forward
start: 138
end: 191
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1737841
display id: annotation1737841
version: 1
top level: T.OnRFP
location: https://synbiohub.org/public/igem/BBa_J01022/annotation1737841/range1737841/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J01022/annotation1737841/range1737841/1
type: Range
persistent identity: range1737841
display id: range1737841
version: 1
top level: T.OnRFP
direction: direction/forward
start: 200
end: 211
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1737848
display id: annotation1737848
version: 1
top level: T.OnRFP
location: https://synbiohub.org/public/igem/BBa_J01022/annotation1737848/range1737848/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J01022/annotation1737848/range1737848/1
type: Range
persistent identity: range1737848
display id: range1737848
version: 1
top level: T.OnRFP
direction: direction/forward
start: 218
end: 898
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1737854
display id: annotation1737854
version: 1
top level: T.OnRFP
location: https://synbiohub.org/public/igem/BBa_J01022/annotation1737854/range1737854/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J01022/annotation1737854/range1737854/1
type: Range
persistent identity: range1737854
display id: range1737854
version: 1
top level: T.OnRFP
direction: direction/forward
start: 932
end: 1011
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1737864
display id: annotation1737864
version: 1
top level: T.OnRFP
location: https://synbiohub.org/public/igem/BBa_J01022/annotation1737864/range1737864/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J01022/annotation1737864/range1737864/1
type: Range
persistent identity: range1737864
display id: range1737864
version: 1
top level: T.OnRFP
direction: direction/forward
start: 1020
end: 1060
orientation: inline

BBa_J01023
type: ComponentDefinition
persistent identity: BBa J01023
display id: BBa_J01023
version: 1
was generated by: iGEM to SBOL conversion
description: [pRM TraJR] = [pRM][StrRBS][TraJR]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: [pRM][StrRBS][TraJR] also internally referred to as i12341
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J01021
component: BBa_I12007
sequence annotation: BBa_I12007
sequence annotation: BBa_J01021
sequence: https://synbiohub.org/public/igem/BBa_J01023_sequence/1

BBa_J01021
type: Component
persistent identity: component2219283
display id: component2219283
version: 1
top level: BBa_J01023
definition: StrRBS.Tra
access: public

BBa_I12007
type: Component
persistent identity: component2219278
display id: component2219278
version: 1
top level: BBa_J01023
definition: Prm +
access: public

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation2219278
display id: annotation2219278
version: 1
top level: BBa_J01023
location: https://synbiohub.org/public/igem/BBa_J01023/annotation2219278/range2219278/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_J01023/annotation2219278/range2219278/1
type: Range
persistent identity: range2219278
display id: range2219278
version: 1
top level: BBa_J01023
direction: direction/reverse
start: 1
end: 82
orientation: inline

BBa_J01021
type: SequenceAnnotation
persistent identity: annotation2219283
display id: annotation2219283
version: 1
top level: BBa_J01023
location: https://synbiohub.org/public/igem/BBa_J01023/annotation2219283/range2219283/1
component: BBa_J01021

https://synbiohub.org/public/igem/BBa_J01023/annotation2219283/range2219283/1
type: Range
persistent identity: range2219283
display id: range2219283
version: 1
top level: BBa_J01023
direction: direction/reverse
start: 91
end: 486
orientation: inline

Key 1
type: ComponentDefinition
persistent identity: BBa J01008
display id: BBa_J01008
version: 1
was generated by: iGEM to SBOL conversion
description: Riboregulator key 1
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Biobricked version of Isaacs' riboregulator trans activating key, taR12
discontinued: false
dominant: true
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01008_sequence/1

StrRBS.Tra
type: ComponentDefinition
persistent identity: BBa J01021
display id: BBa_J01021
version: 1
was generated by: iGEM to SBOL conversion
description: [StrRBS][TraJR]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: [StrRBS][TraJR]
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_J01001
sequence annotation: BBa_B0034
sequence annotation: BBa_J01001
sequence: https://synbiohub.org/public/igem/BBa_J01021_sequence/1

BBa_B0034
type: Component
persistent identity: component2218193
display id: component2218193
version: 1
top level: StrRBS.Tra
definition: BBa_B0034
access: public

BBa_J01001
type: Component
persistent identity: component2218195
display id: component2218195
version: 1
top level: StrRBS.Tra
definition: TraJR
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2218193
display id: annotation2218193
version: 1
top level: StrRBS.Tra
location: https://synbiohub.org/public/igem/BBa_J01021/annotation2218193/range2218193/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J01021/annotation2218193/range2218193/1
type: Range
persistent identity: range2218193
display id: range2218193
version: 1
top level: StrRBS.Tra
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J01001
type: SequenceAnnotation
persistent identity: annotation2218195
display id: annotation2218195
version: 1
top level: StrRBS.Tra
location: https://synbiohub.org/public/igem/BBa_J01021/annotation2218195/range2218195/1
component: BBa_J01001

https://synbiohub.org/public/igem/BBa_J01021/annotation2218195/range2218195/1
type: Range
persistent identity: range2218195
display id: range2218195
version: 1
top level: StrRBS.Tra
direction: direction/reverse
start: 19
end: 396
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lambda Prm promoter (OR-3 obliterated)
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
star: true
creator: Hans
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: -35
sequence annotation: mutate to obliterate OR3
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
top level: Prm +
direction: direction/forward
start: 59
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

TraJR
type: ComponentDefinition
persistent identity: BBa J01001
display id: BBa_J01001
version: 1
was generated by: iGEM to SBOL conversion
description: TraJr protein (controls conjugative transfer in RP4 plasmid)
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
mutable description: TraJR controls conjugative transfer in R plasmid
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: It's complicated
status: status/Planning
bookmark: false
star: true
creator: Berkeley iGEM 2005
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: TraJr
sequence: https://synbiohub.org/public/igem/BBa_J01001_sequence/1

TraJr
type: SequenceAnnotation
persistent identity: annotation1891611
display id: annotation1891611
version: 1
top level: TraJR
location: https://synbiohub.org/public/igem/BBa_J01001/annotation1891611/range1891611/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J01001/annotation1891611/range1891611/1
type: Range
persistent identity: range1891611
display id: range1891611
version: 1
top level: TraJR
direction: direction/forward
start: 1
end: 378
orientation: inline

https://synbiohub.org/public/igem/BBa_J01025_sequence/1
type: Sequence
persistent identity: BBa J01025 sequence
display id: BBa_J01025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccatactagagacccaaatccaggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01020_sequence/1
type: Sequence
persistent identity: BBa J01020 sequence
display id: BBa_J01020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccatactagagacccaaatccaggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01024_sequence/1
type: Sequence
persistent identity: BBa J01024 sequence
display id: BBa_J01024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01006_sequence/1
type: Sequence
persistent identity: BBa J01006 sequence
display id: BBa_J01006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgcca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01022_sequence/1
type: Sequence
persistent identity: BBa J01022 sequence
display id: BBa_J01022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01023_sequence/1
type: Sequence
persistent identity: BBa J01023 sequence
display id: BBa_J01023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01008_sequence/1
type: Sequence
persistent identity: BBa J01008 sequence
display id: BBa_J01008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaatccaggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01021_sequence/1
type: Sequence
persistent identity: BBa J01021 sequence
display id: BBa_J01021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12007_sequence/1
type: Sequence
persistent identity: BBa I12007 sequence
display id: BBa_I12007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01001_sequence/1
type: Sequence
persistent identity: BBa J01001 sequence
display id: BBa_J01001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgatgaaaccaagccaaccaggaagggcagcccacctatcaaggtgtactgccttccagacgaacgaagagcgattgaggaaaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcggccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgagctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaactctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcctcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtcatgatgggcgtggtccgcccgagggcagagccatgataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z