LOCUS       BBa_J01027              1092 bp    DNA     linear       16-May-2024
DEFINITION  [pspoIIe YFP] = [pspoIIe][RBS][YFP][DblTerminator]
ACCESSION   BBa_J01027
VERSION     BBa_J01027.1  
FEATURES             Location/Qualifiers
     promoter        1..206
                     /label=BBa_J01005
     misc_feature    219..222
                     /label=conserved
     RBS             215..226
                     /label=BBa_B0034
     CDS             233..955
                     /label=BBa_E0030
     stem_loop       975..1018
                     /label=stem_loop
     misc_feature    964..1043
                     /label=BBa_B0010
     terminator      964..1043
                     /label=BBa_B0010
     stem_loop       1059..1078
                     /label=T7 TE
     misc_feature    1085..1085
                     /label=stop
     polyA_site      1079..1092
                     /label=polya
     misc_feature    1052..1092
                     /label=BBa_B0012
     terminator      1052..1092
                     /label=BBa_B0012
ORIGIN
        1 cgggtcatcc taacaaatcg gtttctcttg cagaagccga tttttttcat ttttagacaa
       61 cattccggaa attcttttca taaacgaata tcaaggcaga aaccgtcgaa gatttctttg
      121 gtattgttac cttcttttga caaaatccta tctgtgcttt cgctataatg acaggcaacg
      181 aatataacag gtgggagatg agaggatact agagaaagag gagaaatact agatggtgag
      241 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      301 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      361 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      421 caccttcggc tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga
      481 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      541 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      601 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      661 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      721 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      781 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      841 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      901 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aataatacta
      961 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1021 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1081 tctgcgttta ta
//