LOCUS       BBa_J01031              1726 bp    DNA     linear       14-May-2024
DEFINITION  [OnLock??][spo0A][DblTerminator][pspoIIE YFP]
ACCESSION   BBa_J01031
VERSION     BBa_J01031.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..102
                     /label=BBa_J01011
     misc_feature    1..102
                     /label=BBa_J01030
     CDS             867..1589
                     /label=BBa_E0030
     CDS             109..489
                     /label=BBa_J01004
     stem_loop       509..552
                     /label=stem_loop
     misc_feature    498..577
                     /label=BBa_B0010
     terminator      498..577
                     /label=BBa_B0010
     stem_loop       593..612
                     /label=T7 TE
     misc_feature    619..619
                     /label=stop
     polyA_site      613..626
                     /label=polya
     misc_feature    586..626
                     /label=BBa_B0012
     terminator      586..626
                     /label=BBa_B0012
     misc_feature    109..626
                     /label=BBa_J01028
     promoter        635..840
                     /label=BBa_J01005
     misc_feature    853..856
                     /label=conserved
     RBS             849..860
                     /label=BBa_B0034
     CDS             867..1589
                     /label=BBa_E0030
     stem_loop       1609..1652
                     /label=stem_loop
     misc_feature    1598..1677
                     /label=BBa_B0010
     terminator      1598..1677
                     /label=BBa_B0010
     stem_loop       1693..1712
                     /label=T7 TE
     misc_feature    1719..1719
                     /label=stop
     polyA_site      1713..1726
                     /label=polya
     misc_feature    1686..1726
                     /label=BBa_B0012
     terminator      1686..1726
                     /label=BBa_B0012
     misc_feature    635..1726
                     /label=BBa_J01027
     misc_feature    109..1726
                     /label=BBa_J01029
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctctag tgattgggtt caatagagga gatactagat gagcagccag
      121 cctgaaccaa agaagaaaaa tctcgacgcg agcatcacaa gcattatcca tgaaatcggc
      181 gtcccagccc atattaaagg ctatctctat ctgcgcgaag caatctcaat ggtatacaat
      241 gacatcgaat tgctcggcag cattacaaaa gtcctctatc cggacatcgc caaaaaattt
      301 aacacaaccg caagccgtgt agaaagagcg atccgccatg caattgaagt ggcatggagc
      361 agaggaaaca ttgattccat ttcctcgttg tttggttata ctgtcagcat gacaaaagct
      421 aaacctacca acagtgaatt tattgcaatg gttgcggata agctgaggtt agagcataag
      481 gcttcttaat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      541 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      601 ttcgggtggg cctttctgcg tttatatact agagcgggtc atcctaacaa atcggtttct
      661 cttgcagaag ccgatttttt tcatttttag acaacattcc ggaaattctt ttcataaacg
      721 aatatcaagg cagaaaccgt cgaagatttc tttggtattg ttaccttctt ttgacaaaat
      781 cctatctgtg ctttcgctat aatgacaggc aacgaatata acaggtggga gatgagagga
      841 tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg
      901 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      961 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1021 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct
     1081 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag
     1141 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1201 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1261 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1321 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1381 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1441 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     1501 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1561 tcggcatgga cgagctgtac aagtaataat actagagcca ggcatcaaat aaaacgaaag
     1621 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1681 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//