LOCUS       BBa_J01032              2105 bp    DNA     linear       14-May-2024
DEFINITION  [OriTR][OnLock??][spo0A][DblTerminator][pspoIIE YFP]
ACCESSION   BBa_J01032
VERSION     BBa_J01032.1  
FEATURES             Location/Qualifiers
     oriT            1..371
                     /label=BBa_J01003
     misc_feature    380..398
                     /label=TetR 1
     misc_feature    380..433
                     /label=BBa_R0040
     promoter        399..404
                     /label=-35
     misc_feature    405..423
                     /label=TetR 2
     promoter        422..427
                     /label=-10
     promoter        380..433
                     /label=BBa_R0040
     misc_feature    442..481
                     /label=BBa_J01011
     misc_feature    380..481
                     /label=BBa_J01030
     CDS             1246..1968
                     /label=BBa_E0030
     CDS             488..868
                     /label=BBa_J01004
     stem_loop       888..931
                     /label=stem_loop
     misc_feature    877..956
                     /label=BBa_B0010
     terminator      877..956
                     /label=BBa_B0010
     stem_loop       972..991
                     /label=T7 TE
     misc_feature    998..998
                     /label=stop
     polyA_site      992..1005
                     /label=polya
     misc_feature    965..1005
                     /label=BBa_B0012
     terminator      965..1005
                     /label=BBa_B0012
     misc_feature    488..1005
                     /label=BBa_J01028
     promoter        1014..1219
                     /label=BBa_J01005
     misc_feature    1232..1235
                     /label=conserved
     RBS             1228..1239
                     /label=BBa_B0034
     CDS             1246..1968
                     /label=BBa_E0030
     stem_loop       1988..2031
                     /label=stem_loop
     misc_feature    1977..2056
                     /label=BBa_B0010
     terminator      1977..2056
                     /label=BBa_B0010
     stem_loop       2072..2091
                     /label=T7 TE
     misc_feature    2098..2098
                     /label=stop
     polyA_site      2092..2105
                     /label=polya
     misc_feature    2065..2105
                     /label=BBa_B0012
     terminator      2065..2105
                     /label=BBa_B0012
     misc_feature    1014..2105
                     /label=BBa_J01027
     misc_feature    488..2105
                     /label=BBa_J01029
     misc_feature    380..2105
                     /label=BBa_J01031
ORIGIN
        1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
       61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
      121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
      181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
      241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
      301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
      361 gcagcggaaa atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag
      421 agatactgag cactactaga gaactagaat cacctctagt gattgggttc aatagaggag
      481 atactagatg agcagccagc ctgaaccaaa gaagaaaaat ctcgacgcga gcatcacaag
      541 cattatccat gaaatcggcg tcccagccca tattaaaggc tatctctatc tgcgcgaagc
      601 aatctcaatg gtatacaatg acatcgaatt gctcggcagc attacaaaag tcctctatcc
      661 ggacatcgcc aaaaaattta acacaaccgc aagccgtgta gaaagagcga tccgccatgc
      721 aattgaagtg gcatggagca gaggaaacat tgattccatt tcctcgttgt ttggttatac
      781 tgtcagcatg acaaaagcta aacctaccaa cagtgaattt attgcaatgg ttgcggataa
      841 gctgaggtta gagcataagg cttcttaata ctagagccag gcatcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcgggtca
     1021 tcctaacaaa tcggtttctc ttgcagaagc cgattttttt catttttaga caacattccg
     1081 gaaattcttt tcataaacga atatcaaggc agaaaccgtc gaagatttct ttggtattgt
     1141 taccttcttt tgacaaaatc ctatctgtgc tttcgctata atgacaggca acgaatataa
     1201 caggtgggag atgagaggat actagagaaa gaggagaaat actagatggt gagcaagggc
     1261 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1321 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1381 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     1441 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc
     1501 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1561 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1621 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1681 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1741 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1801 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1861 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1921 accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata ctagagccag
     1981 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2041 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2101 ttata
//