##gff-version 3
##sequence-region BBa_J01032 1 2105
BBa_J01032	.	engineered_region	380	2105	.	+	0	ID=BBa_J01031;Name=BBa_J01031
BBa_J01032	.	engineered_region	380	481	.	+	0	ID=BBa_J01030;Name=BBa_J01030;Parent=BBa_J01031
BBa_J01032	.	promoter	380	433	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J01030
BBa_J01032	.	promoter	399	404	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J01032	.	non_covalent_binding_site	405	423	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J01032	.	engineered_region	380	433	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J01032	.	non_covalent_binding_site	380	398	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J01032	.	promoter	422	427	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J01032	.	mature_transcript_region	442	481	.	+	0	ID=BBa_J01011;Name=BBa_J01011;Parent=BBa_J01030
BBa_J01032	.	engineered_region	488	2105	.	+	0	ID=BBa_J01029;Name=BBa_J01029;Parent=BBa_J01031
BBa_J01032	.	engineered_region	488	1005	.	+	0	ID=BBa_J01028;Name=BBa_J01028;Parent=BBa_J01029
BBa_J01032	.	terminator	877	956	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01028
BBa_J01032	.	stem_loop	888	931	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01032	.	engineered_region	877	956	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01032	.	terminator	965	1005	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01028
BBa_J01032	.	stop_codon	998	998	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01032	.	engineered_region	965	1005	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01032	.	stem_loop	972	991	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01032	.	polyA_site	992	1005	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01032	.	CDS	488	868	.	+	0	ID=BBa_J01004;Name=BBa_J01004;Parent=BBa_J01028
BBa_J01032	.	engineered_region	1014	2105	.	+	0	ID=BBa_J01027;Name=BBa_J01027;Parent=BBa_J01029
BBa_J01032	.	promoter	1014	1219	.	+	0	ID=BBa_J01005;Name=BBa_J01005;Parent=BBa_J01027
BBa_J01032	.	ribosome_entry_site	1228	1239	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J01027
BBa_J01032	.	conserved_region	1232	1235	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J01032	.	terminator	2065	2105	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J01027
BBa_J01032	.	stop_codon	2098	2098	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J01032	.	engineered_region	2065	2105	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J01032	.	stem_loop	2072	2091	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J01032	.	polyA_site	2092	2105	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J01032	.	CDS	1246	1968	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_J01027
BBa_J01032	.	terminator	1977	2056	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J01027
BBa_J01032	.	stem_loop	1988	2031	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J01032	.	engineered_region	1977	2056	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J01032	.	CDS	1246	1968	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_J01031
BBa_J01032	.	oriT	1	371	.	+	0	ID=BBa_J01003;Name=BBa_J01003
>BBa_J01032
gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggct
tggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgtt
gagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgccc
ggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatg
gatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaatactagagtccctatcagtgatag
agattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctctagtgattgggttcaata
gaggagatactagatgagcagccagcctgaaccaaagaagaaaaatctcgacgcgagcatcacaagcattatccatgaa
atcggcgtcccagcccatattaaaggctatctctatctgcgcgaagcaatctcaatggtatacaatgacatcgaattgc
tcggcagcattacaaaagtcctctatccggacatcgccaaaaaatttaacacaaccgcaagccgtgtagaaagagcgat
ccgccatgcaattgaagtggcatggagcagaggaaacattgattccatttcctcgttgtttggttatactgtcagcatg
acaaaagctaaacctaccaacagtgaatttattgcaatggttgcggataagctgaggttagagcataaggcttcttaat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcgggtcatcctaac
aaatcggtttctcttgcagaagccgatttttttcatttttagacaacattccggaaattcttttcataaacgaatatca
aggcagaaaccgtcgaagatttctttggtattgttaccttcttttgacaaaatcctatctgtgctttcgctataatgac
aggcaacgaatataacaggtgggagatgagaggatactagagaaagaggagaaatactagatggtgagcaagggcgagg
agctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcga
gggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccc
accctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttca
agtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccga
ggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg
gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtga
acttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcga
cggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgat
cacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata