LOCUS       BBa_J01051              1077 bp    DNA     linear       15-May-2024
DEFINITION  [DblTerminator][OnCFP]
ACCESSION   BBa_J01051
VERSION     BBa_J01051.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..156
                     /label=TetR 1
     misc_feature    138..191
                     /label=BBa_R0040
     promoter        157..162
                     /label=-35
     misc_feature    163..181
                     /label=TetR 2
     promoter        180..185
                     /label=-10
     promoter        138..191
                     /label=BBa_R0040
     misc_feature    204..207
                     /label=conserved
     RBS             200..211
                     /label=BBa_B0034
     CDS             218..940
                     /label=BBa_E0020
     stem_loop       960..1003
                     /label=stem_loop
     misc_feature    949..1028
                     /label=BBa_B0010
     terminator      949..1028
                     /label=BBa_B0010
     stem_loop       1044..1063
                     /label=T7 TE
     misc_feature    1070..1070
                     /label=stop
     polyA_site      1064..1077
                     /label=polya
     misc_feature    1037..1077
                     /label=BBa_B0012
     terminator      1037..1077
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
      181 atactgagca ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct
      241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
      301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
      421 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      541 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      601 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag
      661 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
      721 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      781 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      841 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      901 cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//