LOCUS       BBa_J01054              3144 bp    DNA     linear       14-May-2024
DEFINITION  [pBad/araC][StrRBS][TraJF][DblTerminator][OnCFP]
ACCESSION   BBa_J01054
VERSION     BBa_J01054.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     promoter        2298..2428
                     /label=Pbad
     promoter        2261..2332
                     /label=PC
     misc_feature    1219..2297
                     /label=AraC
     promoter        1219..2428
                     /label=BBa_I0500
     misc_feature    2441..2444
                     /label=conserved
     RBS             2437..2448
                     /label=BBa_B0034
     CDS             2455..3144
                     /label=BBa_J01000
     misc_feature    1219..3144
                     /label=BBa_J01053
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtt atgacaactt gacggctaca tcattcactt tttcttcaca
     1261 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
     1321 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
     1381 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
     1441 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
     1501 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
     1561 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
     1621 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
     1681 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
     1741 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
     1801 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
     1861 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
     1921 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
     1981 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
     2041 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
     2101 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
     2161 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
     2221 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     2281 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     2341 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt
     2401 ttctccatac ccgttttttt gggctagcta ctagagaaag aggagaaata ctagatgtat
     2461 ccgatggatc gtattcaaca aaaacatgct cgtcaaatag atctgctgga aaatctgacg
     2521 gcagttattc aggattatcc aaatccagcc tgtatcaggg acgaaactgg aaaatttatt
     2581 ttttgcaata cgctgtttca tgagtcattt cttacacaag atcaaagtgc tgaaaaatgg
     2641 cttctgtcgc agagagattt ttgtgaattg atctctgtca cagagatgga agcatatagg
     2701 aatgagcata cgcatcttaa tcttgtagag gatgttttta ttcagaatag attctggaca
     2761 atatctgtcc agtcatttct taatggacac agaaatatta ttctgtggca attttatgat
     2821 gctgctcatg ttcgtcataa agacagttat aatcaaaaaa cgattgtcag tgatgatatc
     2881 agaaatataa tcagaagaat gagtgatgat tcttctgtat catcatatgt aaatgatgtc
     2941 ttttacttat atagcaccgg aatcagtcat aatgctatag caagaatatt aaatatatcc
     3001 atctccacat caaagaaaca cgcatctctg atatgcgact acttctctgt ttctaataaa
     3061 gatgagttaa ttatcttact ctacaataaa aagtttattt attatttata cgagaaggct
     3121 atgtgtatca taaatacgcg ttaa
//