BBa_J01009
1
Key 2
Riboregulator Key 2
2005-11-05T12:00:00Z
2015-08-31T04:08:12Z
Another variant of biobricked version of Isaacs' riboregulator trans activating key, taR12, only change is in the address region.
true
false
_13_
0
395
13
Discontinued
false
false
Golden Bear
BBa_J01055
1
KP3.Key2
OnKey2 = [Key promoter KP3][Key 2]
2005-11-05T12:00:00Z
2015-08-31T04:08:12Z
Key 2 under the key promoter.
false
false
_13_
0
395
13
Not in stock
false
false
Golden Bear
component2218197
1
BBa_J01009
component2218196
1
BBa_J01006
annotation2218197
1
BBa_J01009
range2218197
1
68
161
annotation2218196
1
BBa_J01006
range2218196
1
1
59
BBa_J01006
1
KP3
Key Promoter absorbs 3
2005-10-31T12:00:00Z
2015-08-31T04:08:11Z
Promoter for transcribing keys to act on locks based on Isaacs, Collins, et. al: <a href="http://www.nature.com/nbt/journal/v22/n7/full/nbt986.html;jsessionid=464FF968289CFEAE2031D9BC81CDF6EC">
"Engineered riboregulators enable post-transcriptional control of gene expression"</a>
KP3 absorbs three nucleotides from mixed site ("TACTAGAG"), so that the key has a 5 nucleotide spacer region (i.e. "TAGAG") between the transcription start site and the first nucleotide of the key.
We are still looking into what the optimal size for the spacer region is. See also KP2 (BBa_J01007), and Key1 (BBa_J01008), Key2 (BBa_J01009), Lock1 (BBa_J01010), and Lock2 (BBa_J01011).
false
false
_13_
0
395
13
Not in stock
false
false
Golden Bear
BBa_J01006_sequence
1
tcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgcca
BBa_J01055_sequence
1
tcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccatactagagacccaatcactggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctcttgaaagccagattattaatccggctt
BBa_J01009_sequence
1
acccaatcactggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctcttgaaagccagattattaatccggctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z