LOCUS BBa_J01056 3157 bp DNA linear 22-Nov-2024 DEFINITION Non mobilizable TraJF, OnCFP, Key2 = [pBad/araC TraJF][DblTerminator][OnCFP][OnKey2] ACCESSION BBa_J01056 VERSION BBa_J01056.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1926 /label=BBa_J01000 stem_loop 1946..1989 /label=stem_loop misc_feature 1935..2014 /label=BBa_B0010 terminator 1935..2014 /label=BBa_B0010 stem_loop 2030..2049 /label=T7 TE misc_feature 2056..2056 /label=stop polyA_site 2050..2063 /label=polya misc_feature 2023..2063 /label=BBa_B0012 terminator 2023..2063 /label=BBa_B0012 misc_feature 2072..2090 /label=TetR 1 misc_feature 2072..2125 /label=BBa_R0040 promoter 2091..2096 /label=-35 misc_feature 2097..2115 /label=TetR 2 promoter 2114..2119 /label=-10 promoter 2072..2125 /label=BBa_R0040 misc_feature 2138..2141 /label=conserved RBS 2134..2145 /label=BBa_B0034 CDS 2152..2874 /label=BBa_E0020 stem_loop 2894..2937 /label=stem_loop misc_feature 2883..2962 /label=BBa_B0010 terminator 2883..2962 /label=BBa_B0010 stem_loop 2978..2997 /label=T7 TE misc_feature 3004..3004 /label=stop polyA_site 2998..3011 /label=polya misc_feature 2971..3011 /label=BBa_B0012 terminator 2971..3011 /label=BBa_B0012 promoter 3020..3078 /label=BBa_J01006 misc_feature 3087..3157 /label=BBa_J01009 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgt atccgatgga tcgtattcaa 1261 caaaaacatg ctcgtcaaat agatctgctg gaaaatctga cggcagttat tcaggattat 1321 ccaaatccag cctgtatcag ggacgaaact ggaaaattta ttttttgcaa tacgctgttt 1381 catgagtcat ttcttacaca agatcaaagt gctgaaaaat ggcttctgtc gcagagagat 1441 ttttgtgaat tgatctctgt cacagagatg gaagcatata ggaatgagca tacgcatctt 1501 aatcttgtag aggatgtttt tattcagaat agattctgga caatatctgt ccagtcattt 1561 cttaatggac acagaaatat tattctgtgg caattttatg atgctgctca tgttcgtcat 1621 aaagacagtt ataatcaaaa aacgattgtc agtgatgata tcagaaatat aatcagaaga 1681 atgagtgatg attcttctgt atcatcatat gtaaatgatg tcttttactt atatagcacc 1741 ggaatcagtc ataatgctat agcaagaata ttaaatatat ccatctccac atcaaagaaa 1801 cacgcatctc tgatatgcga ctacttctct gtttctaata aagatgagtt aattatctta 1861 ctctacaata aaaagtttat ttattattta tacgagaagg ctatgtgtat cataaatacg 1921 cgttaatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 1981 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 2041 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc 2101 tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta gatggtgagc 2161 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgtg 2221 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 2281 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 2341 accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 2401 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 2461 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 2521 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 2581 tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag 2641 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 2701 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 2761 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 2821 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag 2881 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2941 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3001 ctgcgtttat atactagagt cagaaaatta ttttaaattt cctcttgtca ggccggaata 3061 actccctata atgcgccata ctagagaccc aatcactgga ggtgaatcta gtaggtggtt 3121 aatgaaaatt aacttactta ctagaaatat ctcttga //