BBa_J01056
type: ComponentDefinition
persistent identity: BBa J01056
display id: BBa_J01056
version: 1
was generated by: iGEM to SBOL conversion
description: Non mobilizable TraJF, OnCFP, Key2 = [pBad/araC TraJF][DblTerminator][OnCFP][OnKey2]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: true
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0040
component: BBa_B0010
component: BBa_E0020
component: BBa_J01006
component: BBa_B0012
component: BBa_J01009
component: BBa_I0500
component: BBa_J01000
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_J01000
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_E0020
sequence annotation: BBa_J01006
sequence annotation: BBa_J01009
sequence: https://synbiohub.org/public/igem/BBa_J01056_sequence/1

BBa_B0034
type: Component
persistent identity: component1745676
display id: component1745676
version: 1
top level: BBa_J01056
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1745668
display id: component1745668
version: 1
top level: BBa_J01056
definition: p(tetR)
access: public

BBa_B0010
type: Component
persistent identity: component1745683
display id: component1745683
version: 1
top level: BBa_J01056
definition: BBa_B0010
access: public

BBa_E0020
type: Component
persistent identity: component1745678
display id: component1745678
version: 1
top level: BBa_J01056
definition: ecfp
access: public

BBa_J01006
type: Component
persistent identity: component1745698
display id: component1745698
version: 1
top level: BBa_J01056
definition: KP3
access: public

BBa_B0012
type: Component
persistent identity: component1745693
display id: component1745693
version: 1
top level: BBa_J01056
definition: BBa_B0012
access: public

BBa_J01009
type: Component
persistent identity: component1745700
display id: component1745700
version: 1
top level: BBa_J01056
definition: Key 2
access: public

BBa_I0500
type: Component
persistent identity: component1745631
display id: component1745631
version: 1
top level: BBa_J01056
definition: pBad/araC
access: public

BBa_J01000
type: Component
persistent identity: component1745638
display id: component1745638
version: 1
top level: BBa_J01056
definition: TraJF
access: public

BBa_B0034
type: Component
persistent identity: component1745636
display id: component1745636
version: 1
top level: BBa_J01056
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1745653
display id: component1745653
version: 1
top level: BBa_J01056
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1745643
display id: component1745643
version: 1
top level: BBa_J01056
definition: BBa_B0010
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1745631
display id: annotation1745631
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745631/range1745631/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J01056/annotation1745631/range1745631/1
type: Range
persistent identity: range1745631
display id: range1745631
version: 1
top level: BBa_J01056
direction: direction/forward
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1745636
display id: annotation1745636
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745636/range1745636/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J01056/annotation1745636/range1745636/1
type: Range
persistent identity: range1745636
display id: range1745636
version: 1
top level: BBa_J01056
direction: direction/forward
start: 1219
end: 1230
orientation: inline

BBa_J01000
type: SequenceAnnotation
persistent identity: annotation1745638
display id: annotation1745638
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745638/range1745638/1
component: BBa_J01000

https://synbiohub.org/public/igem/BBa_J01056/annotation1745638/range1745638/1
type: Range
persistent identity: range1745638
display id: range1745638
version: 1
top level: BBa_J01056
direction: direction/forward
start: 1237
end: 1926
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1745643
display id: annotation1745643
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745643/range1745643/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J01056/annotation1745643/range1745643/1
type: Range
persistent identity: range1745643
display id: range1745643
version: 1
top level: BBa_J01056
direction: direction/forward
start: 1935
end: 2014
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1745653
display id: annotation1745653
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745653/range1745653/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J01056/annotation1745653/range1745653/1
type: Range
persistent identity: range1745653
display id: range1745653
version: 1
top level: BBa_J01056
direction: direction/forward
start: 2023
end: 2063
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1745668
display id: annotation1745668
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745668/range1745668/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J01056/annotation1745668/range1745668/1
type: Range
persistent identity: range1745668
display id: range1745668
version: 1
top level: BBa_J01056
direction: direction/forward
start: 2072
end: 2125
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1745676
display id: annotation1745676
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745676/range1745676/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J01056/annotation1745676/range1745676/1
type: Range
persistent identity: range1745676
display id: range1745676
version: 1
top level: BBa_J01056
direction: direction/forward
start: 2134
end: 2145
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1745678
display id: annotation1745678
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745678/range1745678/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_J01056/annotation1745678/range1745678/1
type: Range
persistent identity: range1745678
display id: range1745678
version: 1
top level: BBa_J01056
direction: direction/forward
start: 2152
end: 2874
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1745683
display id: annotation1745683
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745683/range1745683/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J01056/annotation1745683/range1745683/1
type: Range
persistent identity: range1745683
display id: range1745683
version: 1
top level: BBa_J01056
direction: direction/forward
start: 2883
end: 2962
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1745693
display id: annotation1745693
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745693/range1745693/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J01056/annotation1745693/range1745693/1
type: Range
persistent identity: range1745693
display id: range1745693
version: 1
top level: BBa_J01056
direction: direction/forward
start: 2971
end: 3011
orientation: inline

BBa_J01006
type: SequenceAnnotation
persistent identity: annotation1745698
display id: annotation1745698
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745698/range1745698/1
component: BBa_J01006

https://synbiohub.org/public/igem/BBa_J01056/annotation1745698/range1745698/1
type: Range
persistent identity: range1745698
display id: range1745698
version: 1
top level: BBa_J01056
direction: direction/forward
start: 3020
end: 3078
orientation: inline

BBa_J01009
type: SequenceAnnotation
persistent identity: annotation1745700
display id: annotation1745700
version: 1
top level: BBa_J01056
location: https://synbiohub.org/public/igem/BBa_J01056/annotation1745700/range1745700/1
component: BBa_J01009

https://synbiohub.org/public/igem/BBa_J01056/annotation1745700/range1745700/1
type: Range
persistent identity: range1745700
display id: range1745700
version: 1
top level: BBa_J01056
direction: direction/forward
start: 3087
end: 3157
orientation: inline

TraJF
type: ComponentDefinition
persistent identity: BBa J01000
display id: BBa_J01000
version: 1
was generated by: iGEM to SBOL conversion
description: TraJF controls conjugative transfer in F plasmid
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
mutable description: TraJF controls conjugative transfer in F plasmid
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Berkeley iGEM 2005
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J01000_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

KP3
type: ComponentDefinition
persistent identity: BBa J01006
display id: BBa_J01006
version: 1
was generated by: iGEM to SBOL conversion
description: Key Promoter absorbs 3
created: 2005-10-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
mutable description: Promoter for transcribing keys to act on locks based on Isaacs, Collins, et. al: "Engineered riboregulators enable post-transcriptional control of gene expression" KP3 absorbs three nucleotides from mixed site ("TACTAGAG"), so that the key has a 5 nucleotide spacer region (i.e. "TAGAG") between the transcription start site and the first nucleotide of the key. We are still looking into what the optimal size for the spacer region is. See also KP2 (BBa_J01007), and Key1 (BBa_J01008), Key2 (BBa_J01009), Lock1 (BBa_J01010), and Lock2 (BBa_J01011).
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J01006_sequence/1

Key 2
type: ComponentDefinition
persistent identity: BBa J01009
display id: BBa_J01009
version: 1
was generated by: iGEM to SBOL conversion
description: Riboregulator Key 2
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: Another variant of biobricked version of Isaacs' riboregulator trans activating key, taR12, only change is in the address region.
discontinued: true
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01009_sequence/1

https://synbiohub.org/public/igem/BBa_J01056_sequence/1
type: Sequence
persistent identity: BBa J01056 sequence
display id: BBa_J01056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgtatccgatggatcgtattcaacaaaaacatgctcgtcaaatagatctgctggaaaatctgacggcagttattcaggattatccaaatccagcctgtatcagggacgaaactggaaaatttattttttgcaatacgctgtttcatgagtcatttcttacacaagatcaaagtgctgaaaaatggcttctgtcgcagagagatttttgtgaattgatctctgtcacagagatggaagcatataggaatgagcatacgcatcttaatcttgtagaggatgtttttattcagaatagattctggacaatatctgtccagtcatttcttaatggacacagaaatattattctgtggcaattttatgatgctgctcatgttcgtcataaagacagttataatcaaaaaacgattgtcagtgatgatatcagaaatataatcagaagaatgagtgatgattcttctgtatcatcatatgtaaatgatgtcttttacttatatagcaccggaatcagtcataatgctatagcaagaatattaaatatatccatctccacatcaaagaaacacgcatctctgatatgcgactacttctctgtttctaataaagatgagttaattatcttactctacaataaaaagtttatttattatttatacgagaaggctatgtgtatcataaatacgcgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01000_sequence/1
type: Sequence
persistent identity: BBa J01000 sequence
display id: BBa_J01000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtatccgatggatcgtattcaacaaaaacatgctcgtcaaatagatctgctggaaaatctgacggcagttattcaggattatccaaatccagcctgtatcagggacgaaactggaaaatttattttttgcaatacgctgtttcatgagtcatttcttacacaagatcaaagtgctgaaaaatggcttctgtcgcagagagatttttgtgaattgatctctgtcacagagatggaagcatataggaatgagcatacgcatcttaatcttgtagaggatgtttttattcagaatagattctggacaatatctgtccagtcatttcttaatggacacagaaatattattctgtggcaattttatgatgctgctcatgttcgtcataaagacagttataatcaaaaaacgattgtcagtgatgatatcagaaatataatcagaagaatgagtgatgattcttctgtatcatcatatgtaaatgatgtcttttacttatatagcaccggaatcagtcataatgctatagcaagaatattaaatatatccatctccacatcaaagaaacacgcatctctgatatgcgactacttctctgtttctaataaagatgagttaattatcttactctacaataaaaagtttatttattatttatacgagaaggctatgtgtatcataaatacgcgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01006_sequence/1
type: Sequence
persistent identity: BBa J01006 sequence
display id: BBa_J01006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgcca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01009_sequence/1
type: Sequence
persistent identity: BBa J01009 sequence
display id: BBa_J01009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaatcactggaggtgaatctagtaggtggttaatgaaaattaacttacttactagaaatatctcttgaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z