LOCUS       BBa_J01057               968 bp    DNA     linear       22-Nov-2024
DEFINITION  [pRM GFP] = [pRM][StrRBS][GFP][DblTerminator]
ACCESSION   BBa_J01057
VERSION     BBa_J01057.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     RBS             91..105
                     /label=RBS-1\Strong
     RBS             98..102
                     /label=RBS
     misc_feature    91..105
                     /label=BBa_B0030
     RBS             91..105
                     /label=BBa_B0030
     CDS             112..831
                     /label=GFP protein
     CDS             112..831
                     /label=BBa_E0040
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag attaaagagg agaaatacta gatgcgtaaa
      121 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      181 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      241 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      301 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      361 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      421 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      481 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      541 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      601 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      661 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
      721 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      781 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttata
//