LOCUS       BBa_J01059              1888 bp    DNA     linear       23-Dec-2024
DEFINITION  [cI][DblTerminator][pRM GFP]
ACCESSION   BBa_J01059
VERSION     BBa_J01059.1  
FEATURES             Location/Qualifiers
     CDS             4..711
                     /label=cI lambda
     misc_feature    712..744
                     /label=LVA
     misc_feature    751..775
                     /label=Help:Barcodes
     CDS             1..750
                     /label=BBa_C0051
     stem_loop       795..838
                     /label=stem_loop
     misc_feature    784..863
                     /label=BBa_B0010
     terminator      784..863
                     /label=BBa_B0010
     stem_loop       879..898
                     /label=T7 TE
     misc_feature    905..905
                     /label=stop
     polyA_site      899..912
                     /label=polya
     misc_feature    872..912
                     /label=BBa_B0012
     terminator      872..912
                     /label=BBa_B0012
     misc_feature    929..945
                     /label=OR1
     misc_feature    953..969
                     /label=OR2
     promoter        968..973
                     /label=-35
     misc_feature    979..985
                     /label=mutate to obliterate OR3
     promoter        991..996
                     /label=-10
     promoter        921..1002
                     /label=BBa_I12007
     RBS             1011..1025
                     /label=RBS-1\Strong
     RBS             1018..1022
                     /label=RBS
     misc_feature    1011..1025
                     /label=BBa_B0030
     RBS             1011..1025
                     /label=BBa_B0030
     CDS             1032..1751
                     /label=GFP protein
     CDS             1032..1751
                     /label=BBa_E0040
     stem_loop       1771..1814
                     /label=stem_loop
     misc_feature    1760..1839
                     /label=BBa_B0010
     terminator      1760..1839
                     /label=BBa_B0010
     stem_loop       1855..1874
                     /label=T7 TE
     misc_feature    1881..1881
                     /label=stop
     polyA_site      1875..1888
                     /label=polya
     misc_feature    1848..1888
                     /label=BBa_B0012
     terminator      1848..1888
                     /label=BBa_B0012
ORIGIN
        1 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
       61 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag
      121 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
      181 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
      241 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
      301 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
      361 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
      421 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
      481 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
      541 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
      601 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
      661 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgctgcaaac
      721 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      901 tctgcgttta tatactagag gcaaccatta tcaccgccag aggtaaaata gtcaacacgc
      961 acggtgttag atatttataa atagtggtga tagatttaac gttactagag attaaagagg
     1021 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1081 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1141 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1201 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
     1261 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1321 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1381 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1441 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1501 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1561 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1621 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     1681 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1741 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1801 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1861 ccttcgggtg ggcctttctg cgtttata
//